Western Blot: Ribosomal Protein S6/RPS6 Antibody [NB100-1595] - Detection of human and mouse RPS6 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells ...read more
Immunohistochemistry-Paraffin: Ribosomal Protein S6/RPS6 Antibody [NB100-1595] - Section of human lung carcinoma. Antibody: Affinity purified rabbit anti-RPS6 (NB100-1595). Detection: DAB
Immunoprecipitation: Ribosomal Protein S6/RPS6 Antibody [NB100-1595] - Detection of human RPS6 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells ...read more
Loss of SPAG5 and CLUH lead to hyperactivation of mTORC1 signaling. (C) Western blots of WT and SPAG5 ind-KO HeLa cells transfected with siRNA against CLUH or untargeted control siRNA. Cells were induced for 4 days with ...read more
The immunogen recognized by this antibody maps to a region between residue 200 and the C-terminus (residue 249) of human Ribosomal Protein S6 using the numbering given in entry NP_001001.2 (GeneID 6194).
Predicted Species
Rat (100%), Chicken (100%), Xenopus (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RPS6
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Theoretical MW
29 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4 using NB100-1595 in the following applications:
Alternate Names for Ribosomal Protein S6/RPS6 Antibody
Phosphoprotein NP33, 40S ribosomal protein S6
pS6
Ribosomal Protein S6
RPS6
S6
Background
Ribosomal protein S6 (RPS6) is a component of the 40S ribosomal subunit. Ribosomes are composed of a small 40S subunit and a large 60S subunit that function to catalyze protein synthesis. RPS6 is phosphorylated by many cellular kinases including ribosomal protein S6 kinase that is regulated via the mammalian target of rapamycin (mTOR) pathway. RPS6 has been demonstrated to be important in regulating cell size, glucose homeostasis, and translational control of mRNA. Alternate names for RPS6 include 40S ribosomal protein S6, and phosphoprotein NP33.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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