Western Blot: Calnexin Antibody [NB100-1965] - WB analysis of CANX in cell lysates as noted.
Western Blot: Calnexin Antibody [NB100-1965] - Subcellular localization of TM6SF2.Immunoaffinity isolation of ER and Golgi complex from mouse liver. ER and Golgi fractions were prepared from mouse liver microsomes by ...read more
Immunohistochemistry: Calnexin Antibody [NB100-1965] - Calnexin immunostaining in the anterior (AI) and mid (MI) intestine of ctrl (control zebrafish), D.I.O. (diet-induced obesity zebrafish), D.I.O. flw 3,5-T2 (D.I.O. ...read more
Immunocytochemistry/ Immunofluorescence: Calnexin Antibody [NB100-1965] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were ...read more
Immunocytochemistry/ Immunofluorescence: Calnexin Antibody [NB100-1965] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were ...read more
Immunocytochemistry/ Immunofluorescence: Calnexin Antibody [NB100-1965] - Rat FR cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were ...read more
Western Blot: Calnexin Antibody - BSA Free [NB100-1965] - Whole cell lysate or exosome from MDA-MD-231 cells was loaded with 20 ug/lane. 10% SDS-PAGE. Calnexin Antibody (NB100-1965) was used for primary antibody: ...read more
Flow Cytometry: Calnexin Antibody [NB100-1965] - An intracellular stain was performed on rat FR cells with Calnexin Antibody NB100-1965 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% ...read more
Western Blot: Calnexin Antibody [NB100-1965] - A dilution of 1/2000 used in TBS with 0.05% Tween-20. Bands visualized by ECL using goat anti-rabbit HRP at 1:2000.
Immunohistochemistry: Calnexin Antibody [NB100-1965] - IHC analysis of CANX in mouse prostate using DAB with hematoxylin counterstain.
Flow Cytometry: Calnexin Antibody [NB100-1965] - An intracellular stain was performed on Daudi cells with Calnexin Antibody NB100-1965 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% ...read more
Flow Cytometry: Calnexin Antibody [NB100-1965] - An intracellular stain was performed on NIH3T3 cells with Calnexin Antibody NB100-1965 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% ...read more
Simple Western: Calnexin Antibody [NB100-1965] - Simple Western lane view shows a specific band for Calnexin in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more
Western Blot: Calnexin Antibody - BSA Free [NB100-1965] - Hepatic apoB levels (A), liver function tests (B), & relative hepatic levels of selected transcripts encoding proteins involved in activation of fibrosis & ER ...read more
This Calnexin antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry-paraffin embedded sections, Immunoprecipitation and Western Blot. In Western blot a band is observed approx. 97 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Theoretical MW
97 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 4 Reviews rated 4.3 using NB100-1965 in the following applications:
Quail. Predicted to react with dog based on 100% sequence homology.
Packaging, Storage & Formulations
Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified
Alternate Names for Calnexin Antibody - BSA Free
calnexin
CNX
IP90FLJ26570
Major histocompatibility complex class I antigen-binding protein p88
P90
Background
Calnexin is a chaperone protein that assists in protein folding and quality control. Calnexin retains unassembled or unfolded N-linked glycoproteins in the endoplasmic reticulum and ensures that only properly assembled and folded proteins proceed down the secretory pathway. Calnexin antibodies can be used as endoplasmic reticulum markers (ER markers) in immunostaining experiments
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
De Bardi M Pharmacological tuning of microRNAs in FAP-derived Extracellular Vesicles by HDAC 2 inhibitors promotes regeneration and reduces fibrosis in dystrophic muscles bioRxiv 2020-01-01 (WB, Mouse)
WB
Mouse
Corbel S, Sampath S, Schmedt C, Zhang Q COMPOSITIONS, METHODS, AND THERAPEUTIC USES RELATED TO FUSOGENIC PROTEIN MINION United States Patent Application 20190263899 2019-08-29
Turner N, Abeysinghe P, Sadowski P, Mitchell M Cross-species proteomic and microRNA comparison of extracellular vesicles in human milk, cow's milk, and infant formula products: moving towards next generation infant formula products bioRxiv 2023-02-24 (Western Blot, Bovine, Human)
Adamo G, Santonicola P, Picciotto S et al. Microalgae as a novel biofactory for biocompatible and bioactive extracellular vesicles bioRxiv 2023-04-04 (ICC/IF, Human)
Johnson B, Jun D, DeBose-Boyd R Membrane Topology of UbiA Prenyltransferase Domain-Containing Protein-1 (UBIAD1), a Novel Regulator of Cholesterol Homeostasis bioRxiv 2023-03-06 (WB)
Reviews for Calnexin Antibody (NB100-1965) (4)
4.34
Average Rating: 4.3 (Based on 4 reviews)
We have 4 reviews tested in 2 species: Human, Mouse.
Reviews using NB100-1965:
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WB
(3)
IP
(1)
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Human
(3)
Mouse
(1)
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Images
Ratings
Applications
Species
Date
Details
5
reviewed by: Yongkang Yang
WB
Human
10/21/2022
View
Summary
Application
Western Blot
Sample Tested
MDA MB 231 whole cell lysates and conditioned medium
Species
Human
Lot
E-2
2
reviewed by: Lamberto Torralba
IP
Human
03/14/2022
View
Summary
Application
Immunoprecipitation
Sample Tested
B cell lymphoma
Species
Human
Lot
E
Comments
Comments
***Bio-Techne Response: Thank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.***
5
reviewed by: Lamberto Torralba
WB
Human
02/16/2022
View
Summary
Application
Western Blot
Sample Tested
293t HEK and U2OS cells
Species
Human
Lot
E
Comments
Comments
Lane 1: WCL of 5 x 10^6 HEK cells Lane 2: Membrane enrichment of 5 x 10^6 HEK cells Size is around 75 kDA. One clear and specific band.
20 ug of whole cell lysate and exosomal lysate were loaded. Primary was used @ 2 ug/mL. Donkey anti-rabbit-HRP secondary was used @ 1:5000. Entry level (ug sensitivity) ECL reagents were used.
Product General Protocols
View specific protocols for Calnexin Antibody (NB100-1965):
Calnexin - an ER chaperone that folds the cell's glycoproteins Calnexin is an abundant 90kDa chaperone protein that resides in the membrane of the endoplasmic reticulum. Calnexin and the related calreticulin protein function together to ensure the proper folding of glycoproteins. By binding to partially folded... Read full blog post.
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