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Calnexin Antibody - BSA Free

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Western Blot: Calnexin Antibody [NB100-1965] - WB analysis of CANX in cell lysates as noted.
Western Blot: Calnexin Antibody [NB100-1965] - Subcellular localization of TM6SF2.Immunoaffinity isolation of ER and Golgi complex from mouse liver. ER and Golgi fractions were prepared from mouse liver microsomes by ...read more
Immunohistochemistry: Calnexin Antibody [NB100-1965] - Calnexin immunostaining in the anterior (AI) and mid (MI) intestine of ctrl (control zebrafish), D.I.O. (diet-induced obesity zebrafish), D.I.O. flw 3,5-T2 (D.I.O. ...read more
Immunocytochemistry/ Immunofluorescence: Calnexin Antibody [NB100-1965] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were ...read more
Immunocytochemistry/ Immunofluorescence: Calnexin Antibody [NB100-1965] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were ...read more
Immunocytochemistry/ Immunofluorescence: Calnexin Antibody [NB100-1965] - Rat FR cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were ...read more
Western Blot: Calnexin Antibody - BSA Free [NB100-1965] - Whole cell lysate or exosome from MDA-MD-231 cells was loaded with 20 ug/lane. 10% SDS-PAGE. Calnexin Antibody (NB100-1965) was used for primary antibody: ...read more
Flow Cytometry: Calnexin Antibody [NB100-1965] - An intracellular stain was performed on rat FR cells with Calnexin Antibody NB100-1965 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% ...read more
Western Blot: Calnexin Antibody [NB100-1965] - A dilution of 1/2000 used in TBS with 0.05% Tween-20. Bands visualized by ECL using goat anti-rabbit HRP at 1:2000.
Immunohistochemistry: Calnexin Antibody [NB100-1965] - IHC analysis of CANX in mouse prostate using DAB with hematoxylin counterstain.
Flow Cytometry: Calnexin Antibody [NB100-1965] - An intracellular stain was performed on Daudi cells with Calnexin Antibody NB100-1965 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% ...read more
Flow Cytometry: Calnexin Antibody [NB100-1965] - An intracellular stain was performed on NIH3T3 cells with Calnexin Antibody NB100-1965 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% ...read more
Simple Western: Calnexin Antibody [NB100-1965] - Simple Western lane view shows a specific band for Calnexin in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more
Western Blot: Calnexin Antibody - BSA Free [NB100-1965] - Hepatic apoB levels (A), liver function tests (B), & relative hepatic levels of selected transcripts encoding proteins involved in activation of fibrosis & ER ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Av, Bv, Ch, Dr, Gp, Rb, Sh, Xp, Ze, CaSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

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Calnexin Antibody - BSA Free Summary

Immunogen
A synthetic peptide made to an internal region of the canine Calnexin protein (within residues 25-100). [Swiss-Prot P24643]
Localization
Endoplasmic reticulum
Marker
Endoplasmic Reticulum Membrane Marker
Predicted Species
Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CANX
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1-5 ug/ml
  • Immunocytochemistry/ Immunofluorescence 1-5 ug/ml
  • Immunohistochemistry 1:40
  • Immunohistochemistry-Paraffin 1:40
  • Immunoprecipitation 1:100
  • Simple Western 1:25
  • Western Blot 2 ug/ml
Application Notes
This Calnexin antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry-paraffin embedded sections, Immunoprecipitation and Western Blot. In Western blot a band is observed approx. 97 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Theoretical MW
97 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 4 Reviews rated 4.3
using
NB100-1965 in the following applications:

Publications
Read Publications using
NB100-1965 in the following applications:

Reactivity Notes

Quail. Predicted to react with dog based on 100% sequence homology.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Calnexin Antibody - BSA Free

  • calnexin
  • CNX
  • IP90FLJ26570
  • Major histocompatibility complex class I antigen-binding protein p88
  • P90

Background

Calnexin is a chaperone protein that assists in protein folding and quality control. Calnexin retains unassembled or unfolded N-linked glycoproteins in the endoplasmic reticulum and ensures that only properly assembled and folded proteins proceed down the secretory pathway. Calnexin antibodies can be used as endoplasmic reticulum markers (ER markers) in immunostaining experiments

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Calnexin Antibody (NB100-1965)(45)

We have publications tested in 6 confirmed species: Human, Mouse, Bovine, Chinese Hamster, Porcine, Zebrafish.

We have publications tested in 7 applications: Block/Neutralize, IB, ICC/IF, IF/IHC, Simple Western, WB, Western Blot.


Filter By Application
Block/Neutralize
(1)
IB
(1)
ICC/IF
(4)
IF/IHC
(2)
Simple Western
(1)
WB
(19)
Western Blot
(6)
All Applications
Filter By Species
Human
(12)
Mouse
(9)
Bovine
(3)
Chinese Hamster
(1)
Porcine
(1)
Zebrafish
(2)
All Species
Showing Publications 1 - 10 of 45. Show All 45 Publications.
Publications using NB100-1965 Applications Species
Zhu Y, Hu S, Pan X et al. Hepatocyte Sirtuin 6 Protects against Atherosclerosis and Steatohepatitis by Regulating Lipid Homeostasis Cells 2023-08-05 [PMID: 37566087] (Western Blot) Western Blot
Turner NP, Abeysinghe P, Sadowski P, Mitchell MD Omics Analysis of Extracellular Vesicles Recovered from Infant Formula Products and Milk: Towards Personalized Infant Nutrition Molecular nutrition & food research 2023-08-10 [PMID: 37562982] (Western Blot, Bovine, Human) Western Blot Bovine, Human
De Bardi M Pharmacological tuning of microRNAs in FAP-derived Extracellular Vesicles by HDAC 2 inhibitors promotes regeneration and reduces fibrosis in dystrophic muscles bioRxiv 2020-01-01 (WB, Mouse) WB Mouse
Corbel S, Sampath S, Schmedt C, Zhang Q COMPOSITIONS, METHODS, AND THERAPEUTIC USES RELATED TO FUSOGENIC PROTEIN MINION United States Patent Application 20190263899 2019-08-29
Sabrina Garbo, Daniel D'Andrea, Alessio Colantoni, Francesco Fiorentino, Antonello Mai, Andres Ramos, Gian Gaetano Tartaglia, Andrea Tancredi, Marco Tripodi, Cecilia Battistelli m6A modification inhibits miRNAs' intracellular function, favoring their extracellular export for intercellular communication. Cell reports 2024-07-01 [PMID: 38878288]
Renwei Jing, Leijie Zhang, Ruibin Li, Zhongqiu Yang, Jun Song, Qian Wang, Nan Cao, Gang Han, HaiFang Yin Milk‐derived extracellular vesicles functionalized with anti‐tumour necrosis factor‐ alpha nanobody and anti‐microbial peptide alleviate ulcerative colitis in mice Journal of Extracellular Vesicles 2024-06-05 [PMID: 38840457]
Jason A. Weesner, Ida Annunziata, Diantha van de Vlekkert, Camenzind G. Robinson, Yvan Campos, Ashutosh Mishra, Leigh E. Fremuth, Elida Gomero, Huimin Hu, Alessandra d’Azzo Altered GM1 catabolism affects NMDAR-mediated Ca 2+ signaling at ER-PM junctions and increases synaptic spine formation in a GM1-gangliosidosis model Cell reports 2024-06-20 [PMID: 38630590]
Raja Gopoju, Jiayou Wang, Xiaoli Pan, Shuwei Hu, Li Lin, Alyssa Clark, Yanyong Xu, Liya Yin, Xinwen Wang, Yanqiao Zhang Hepatic FOXA3 overexpression prevents Western diet–induced obesity and MASH through TGR5 Journal of Lipid Research 2024-03-04 [PMID: 38447926]
Miira M. Klemetti, Ante B. V. Pettersson, Aafaque Ahmad Khan, Leonardo Ermini, Tyler R. Porter, Michael L. Litvack, Sruthi Alahari, Stacy Zamudio, Nicholas P. Illsley, Hannes Röst, Martin Post, Isabella Caniggia Lipid profile of circulating placental extracellular vesicles during pregnancy identifies foetal growth restriction risk Journal of Extracellular Vesicles 2024-02-14 [PMID: 38353485]
Chen H, Qi X, Faulkner RA et al. Regulated degradation of HMG CoA reductase requires conformational changes in sterol-sensing domain Nature communications 2022-07-25 [PMID: 35879350] (WB, Chinese Hamster) WB Chinese Hamster
Show All 45 Publications.

Reviews for Calnexin Antibody (NB100-1965) (4) 4.34

Average Rating: 4.3
(Based on 4 reviews)
We have 4 reviews tested in 2 species: Human, Mouse.

Reviews using NB100-1965:
Filter by Applications
WB
(3)
IP
(1)
All Applications
Filter by Species
Human
(3)
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Yongkang Yang
WB Human 10/21/2022
View

Summary

ApplicationWestern Blot
Sample TestedMDA MB 231 whole cell lysates and conditioned medium
SpeciesHuman
LotE-2
  2
reviewed by:
Lamberto Torralba
IP Human 03/14/2022
View

Summary

ApplicationImmunoprecipitation
Sample TestedB cell lymphoma
SpeciesHuman
LotE

Comments

Comments***Bio-Techne Response: Thank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.***
  5
reviewed by:
Lamberto Torralba
WB Human 02/16/2022
View

Summary

ApplicationWestern Blot
Sample Tested293t HEK and U2OS cells
SpeciesHuman
LotE

Comments

CommentsLane 1: WCL of 5 x 10^6 HEK cells
Lane 2: Membrane enrichment of 5 x 10^6 HEK cells
Size is around 75 kDA. One clear and specific band.
Western Blot Calnexin NB100-1965
Enlarge
5
reviewed by:
Elizabeth Soberg
WB Mouse 06/13/2018
View

Summary

ApplicationWestern Blot
Sample TestedRAW 264.7 mouse monocyte/macrophage cell line
SpeciesMouse
LotC-3

Comments

Comments20 ug of whole cell lysate and exosomal lysate were loaded.
Primary was used @ 2 ug/mL.
Donkey anti-rabbit-HRP secondary was used @ 1:5000.
Entry level (ug sensitivity) ECL reagents were used.

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Calnexin Antibody (NB100-1965) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Calnexin Products

Research Areas for Calnexin Antibody (NB100-1965)

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Blogs on Calnexin.

Calnexin - an ER chaperone that folds the cell's glycoproteins
Calnexin is an abundant 90kDa chaperone protein that resides in the membrane of the endoplasmic reticulum. Calnexin and the related calreticulin protein function together to ensure the proper folding of glycoproteins. By binding to partially folded...  Read full blog post.

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Recent Reviews

4.3
5
3
4
0
3
0
2
1
1
0

Yongkang Yang
10/21/2022
Application: WB
Species: Human

Lamberto Torralba
03/14/2022
Application: IP
Species: Human

Lamberto Torralba
02/16/2022
Application: WB
Species: Human

Bioinformatics

Gene Symbol CANX
Entrez
Uniprot