![Immunohistochemistry-Paraffin: WT1 Antibody (WT1/857) - Azide and BSA Free [NBP2-47931] - Formalin-fixed, paraffin-embedded Rat kidney stained with WT1 Monoclonal Antibody (WT1/857)](http://images.novusbio.com/fullsize/WT1-Antibody-WT1-857-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47931-img0006.jpg "Immunohistochemistry-Paraffin: WT1 Antibody (WT1/857) - Azide and BSA Free [NBP2-47931] - Formalin-fixed, paraffin-embedded Rat kidney stained with WT1 Monoclonal Antibody (WT1/857)")
| Reactivity | Hu, RtSpecies Glossary |
| Applications | IHC |
| Clone | WT1/857 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1.0 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44606). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | Recombinant full-length human WT1 protein (Uniprot: P19544) |
| Localization | Nuclear and Cytoplasmic |
| Marker | Wilm's Tumor & Mesothelial Marker |
| Specificity | Recognizes a 47-55kDa-tumor suppressor protein, identified as Wilms tumor and mesothelioma. WT1 protein has been identified in proliferative mesothelial cells, malignant mesothelioma, ovarian carcinoma, gonadoblastoma, nephroblastoma, and desmoplastic small round cell tumor. Lung adenocarcinomas rarely stain positive with this antibody. WT1 protein expression in mesothelial cells has become a reliable marker for the diagnosis of mesotheliomas. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | WT1 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1.0 mg/ml |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for WT1 Antibody (NBP2-47931)Find related products by research area.
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Routine WT1 Antibody Screen Uncovers an Exciting New Cancer-Cleaning Enzyme Enzyme antibodies are widely used in both apoptosis and cancer studies, as disruption of the proteins regulating apoptosis is known to lead to formation of tumor cells in certain cancers. We at Novus Biologicals are continually growing our enzyme anti... Read full blog post. |
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| Gene Symbol | WT1 |
![Immunohistochemistry-Paraffin: WT1 Antibody (WT1/857) - Azide and BSA Free [NBP2-47931] - Human Mesothelioma stained with Wilm's Tumor Mouse Monoclonal Antibody (WT1/857).](http://images.novusbio.com/fullsize/WT1-Antibody-WT1-857-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47931-img0001.jpg "Immunohistochemistry-Paraffin: WT1 Antibody (WT1/857) - Azide and BSA Free [NBP2-47931] - Human Mesothelioma stained with Wilm's Tumor Mouse Monoclonal Antibody (WT1/857).")
![Immunohistochemistry-Paraffin: WT1 Antibody (WT1/857) - Azide and BSA Free [NBP2-47931] - Human Testis stained with Wilm's Tumor Mouse Monoclonal Antibody (WT1/857).](http://images.novusbio.com/fullsize/WT1-Antibody-WT1-857-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47931-img0002.jpg "Immunohistochemistry-Paraffin: WT1 Antibody (WT1/857) - Azide and BSA Free [NBP2-47931] - Human Testis stained with Wilm's Tumor Mouse Monoclonal Antibody (WT1/857).")
![Immunohistochemistry-Paraffin: WT1 Antibody (WT1/857) - Azide and BSA Free [NBP2-47931] - Rat Testis stained with Wilm's Tumor Mouse Monoclonal Antibody (WT1/857).](http://images.novusbio.com/fullsize/WT1-Antibody-WT1-857-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47931-img0003.jpg "Immunohistochemistry-Paraffin: WT1 Antibody (WT1/857) - Azide and BSA Free [NBP2-47931] - Rat Testis stained with Wilm's Tumor Mouse Monoclonal Antibody (WT1/857).")
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