![Western Blot: EWSR1 Antibody [NB200-182] - Image shows a specific band for EWSR1 (observed molecular weight ~95 kDa) in HEK293T lysate.](http://images.novusbio.com/fullsize/EWSR1-Antibody-Western-Blot-NB200-182-img0018.jpg "Western Blot: EWSR1 Antibody [NB200-182] - Image shows a specific band for EWSR1 (observed molecular weight ~95 kDa) in HEK293T lysate.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, Simple Western, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1.0 mg/ml |
| Immunogen | A synthetic peptide that maps to a region between residues 100 and 150 of human Ewing sarcoma breakpoint region 1 using the numbering given in SwissProt entry Q01844 (GeneID 2130). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | EWSR1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
| Publication using NB200-182 | Applications | Species |
|---|---|---|
| Davis IJ, Kim JJ, Ozsolak F et al. Oncogenic MITF dysregulation in clear cell sarcoma: defining the MiT family of human cancers. Cancer Cell 2006-06-01 [PMID: 16766266] |
Secondary Antibodies |
Isotype Controls |
Research Areas for EWSR1 Antibody (NB200-182)Find related products by research area.
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![Immunohistochemistry-Paraffin: EWSR1 Antibody [NB200-182] - Detection of human EWS by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-EWS (NB200-182). Detection: DAB](http://images.novusbio.com/fullsize/EWSR1-Antibody-Immunohistochemistry-NB200-182-img0014.jpg "Immunohistochemistry-Paraffin: EWSR1 Antibody [NB200-182] - Detection of human EWS by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-EWS (NB200-182). Detection: DAB")
![Western Blot: EWSR1 Antibody [NB200-182] - Detection of human and mouse EWS by western blot. Samples: Whole cell lysate (5 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-EWS antibody NB200-182 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/EWSR1-Antibody-Western-Blot-NB200-182-img0016.jpg "Western Blot: EWSR1 Antibody [NB200-182] - Detection of human and mouse EWS by western blot. Samples: Whole cell lysate (5 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-EWS antibody NB200-182 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.")
![Immunoprecipitation: EWSR1 Antibody [NB200-182] - Detection of human EWS by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-EWS antibody NB200-182 (lot NB200-182-2) used for IP at 3 ug per reaction. EWS was also immunoprecipitated by a previous lot of this antibody (lot NB200-182-1). For blotting immunoprecipitated EWS, NB200-182 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/EWSR1-Antibody-Immunoprecipitation-NB200-182-img0015.jpg "Immunoprecipitation: EWSR1 Antibody [NB200-182] - Detection of human EWS by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-EWS antibody NB200-182 (lot NB200-182-2) used for IP at 3 ug per reaction. EWS was also immunoprecipitated by a previous lot of this antibody (lot NB200-182-1). For blotting immunoprecipitated EWS, NB200-182 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.")
![Simple Western: EWSR1 Antibody [NB200-182] - Simple Western lane view shows HeLa, HEK293T, and HepG2 whole cell lysate (WCL). A specific band was detected for EWSR1 antibody (NBP1-49701) at approximately 116-140 kDa (as indicated) using 10 ug/mL of EWSR1 antibody. This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/EWSR1-Antibody-Simple-Western-NB200-182-img0017.jpg "Simple Western: EWSR1 Antibody [NB200-182] - Simple Western lane view shows HeLa, HEK293T, and HepG2 whole cell lysate (WCL). A specific band was detected for EWSR1 antibody (NBP1-49701) at approximately 116-140 kDa (as indicated) using 10 ug/mL of EWSR1 antibody. This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")