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EGR1 Antibody [Unconjugated]

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EGR1 was detected in immersion fixed SK‑BR‑3 human breast cancer cell line using Goat Anti-Human EGR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2818) at 5 µg/ml for 3 hours at room ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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EGR1 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human EGR1
Gln282-Ser433
Accession # P18146
Specificity
Detects human EGR1 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
EGR1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 1.7-15 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF2818 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EGR1 Antibody [Unconjugated]

  • AT225Transcription factor ETR103
  • early growth response 1
  • early growth response protein 1
  • EGR1
  • EGR-1
  • G0S30
  • KROX24
  • KROX-24
  • Nerve growth factor-induced protein A
  • NGFIA
  • NGFI-ATranscription factor Zif268
  • TIS8
  • ZIF-268
  • Zinc finger protein Krox-24
  • ZNF225
  • ZNF225Zinc finger protein 225

Background

Early Growth Response 1, (EGR1) belongs to the EGR family of zinc finger transcription factors and regulates the expression of several tumor suppressors. Its expression is induced by diverse signals that initiate growth and differentiation. EGR1 suppresses or activates cell growth depending on the tumor type. Within the region that includes its three zinc finger domains, human EGR1 shares 99% aa sequence identity with mouse and rat EGR1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for EGR1 Antibody (AF2818)(13)

We have publications tested in 2 confirmed species: Human, Chicken.

We have publications tested in 4 applications: IHC, IHC-Fr, IHC-P, Western Blot.


Filter By Application
IHC
(1)
IHC-Fr
(1)
IHC-P
(1)
Western Blot
(1)
All Applications
Filter By Species
Human
(2)
Chicken
(2)
All Species
Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using AF2818 Applications Species
F. G. Duque, C. A. Rodriguez-Saltos, S. Uma, I. Nasir, M. F. Monteros, W. Wilczynski et al. High-frequency hearing in a hummingbird Science Advances 7/17/2020 [PMID: 32832648]
T Song, D Spillmann Transcriptomic analysis reveals cell apoptotic signature modified by heparanase in melanoma cells J. Cell. Mol. Med., 2019-05-02;0(0):. 2019-05-02 [PMID: 31044520] (Western Blot, Human) Western Blot Human
Ronny R. Racine, Nathan A. Manalo, Jessica M.F. Hall, Adnan Dibas, Glen D. Raffel, Mark E. Mummert CD44 induced enhancement of phosphatase activity and calcium influx: Modifications of EGR-1 expression and cell proliferation Biochemistry and Biophysics Reports 7/1/2016 [PMID: 28955875]
Levi Todd, Isabella Palazzo, Natalie Squires, Ninoshka Mendonca, Andy J. Fischer BMP- and TGF beta -signaling regulate the formation of Müller glia-derived progenitor cells in the avian retina Glia 10/1/2017 [PMID: 28703293]
Levi Todd, Natalie Squires, Lilianna Suarez, Andy J. Fischer Jak/Stat signaling regulates the proliferation and neurogenic potential of Müller glia-derived progenitor cells in the avian retina Scientific Reports 10/19/2016 [PMID: 27759082]
Donika Gallina, Isabella Palazzo, Lillia Steffenson, Levi Todd, Andy J. Fischer Wnt/ beta -catenin-signaling and the formation of Müller glia-derived progenitors in the chick retina Developmental Neurobiology 9/1/2016 [PMID: 26663639]
Levi Todd, Leo I. Volkov, Chris Zelinka, Natalie Squires, Andy J. Fischer Heparin-binding EGF-like growth factor (HB-EGF) stimulates the proliferation of Müller glia-derived progenitor cells in avian and murine retinas Molecular and Cellular Neuroscience 11/1/2015 [PMID: 26500021]
Andy J. Fischer, Christopher Zelinka, Donika Gallina, Melissa A. Scott, Levi Todd Reactive microglia and macrophage facilitate the formation of Müller glia-derived retinal progenitors Glia 10/1/2014 [PMID: 24916856]
Myung D, Park Y, Kim N, Chung C, Park H, Kim J, Cho S, Lee W, Lee J, Joo Y Expression of early growth response-1 in colorectal cancer and its relation to tumor cell proliferation and apoptosis. Oncol Rep, 2013-11-29;31(2):788-94. 2013-11-29 [PMID: 24297681] (IHC-P, Human) IHC-P Human
Eric R. Ritchey, Christopher Zelinka, Junhua Tang, Jun Liu, Kimberly A. Code, Simon Petersen-Jones et al. Vision-guided ocular growth in a mutant chicken model with diminished visual acuity Experimental Eye Research 9/1/2012 [PMID: 22824538]
Show All 13 Publications.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Blogs on EGR1.

Taking Biomarker Discovery From 2D to 3D: Increased Biological Activity of EVs Isolated From 3D Prostate Cancer Cultures
Jamshed Arslan, Pharm D, PhD Tissues within the human body are made of a three-dimensional (3D) arrangement of cells working together to perform vital functions. The commonly used 2D monolayer cultures have limited ...  Read full blog post.

Synapsin I: Implicated in synaptic activity across a diverse range of studies
Synapsins are a family of neuronal proteins that are most renowned for their activity in modulating the pre-synaptic terminal.  Synapsin’s behavior is regulated by protein kinases and phosphatases, which alter the way that synapsin’s i...  Read full blog post.

No Monkey Business: APE1 is a Critical DNA Repair Enzyme
APE1 (aka. HAP1, /Ref-1 or APEX) the mammalian ortholog of Escherichia coli Xth is a multifunctional protein possessing both DNA repair and transcriptional regulatory activity. APE1 acts essentially as master regulator of controlling cellular response...  Read full blog post.

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Bioinformatics

Gene Symbol EGR1
Uniprot