WT1 Antibodies
We offer WT1 Antibodies for use in common research applications: ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Intracellular Staining by Flow Cytometry, Peptide ELISA, Sandwich ELISA, Simple Western, Western Blot. Each WT1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our WT1 Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the WT1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our WT1 polyclonal antibodies and browse our WT1 monoclonal antibody catalog.
Alternate Names for WT1 Antibodies
anti-WT1 antibody, anti-AWT1 antibody, anti-NPHS4GUD antibody, anti-WAGR antibody, anti-Wilms tumor 1 antibody, anti-Wilms tumor protein antibody, anti-WIT-2 antibody, anti-WT33 antibody
22 Results for "wt1" in Primary Antibodies
![Western Blot: WT1 Antibody (6F-H2) [NB110-60011] - Hypomethylation of the Intron 1 CpG island results in WT1 expression. RNA was isolated from K562 and U937 cells growing under atmospheric conditions (Normox) and from U937 cells growing in 1% O2 (Hypox) and was analyzed by RT-PCR using primers that span exon 5 of the WT1 mRNA. Ribosomal RNA 36B4 is used as a positive control, and molecular weight markers (MW) are shown. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0119837), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/WT1-Antibody-6F-H2-Western-Blot-NB110-60011-img0007.jpg "Western Blot: WT1 Antibody (6F-H2) [NB110-60011] - Hypomethylation of the Intron 1 CpG island results in WT1 expression. RNA was isolated from K562 and U937 cells growing under atmospheric conditions (Normox) and from U937 cells growing in 1% O2 (Hypox) and was analyzed by RT-PCR using primers that span exon 5 of the WT1 mRNA. Ribosomal RNA 36B4 is used as a positive control, and molecular weight markers (MW) are shown. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0119837), licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: WT1 Antibody (6F-H2) [NB110-60011] - Staining of human kidney glomerulus tissue. Heat mediated antigen retrieval was performed by heating in citrate buffer (pH 6) at 95C for 20 minutes. Image from verified customer review.](http://images.novusbio.com/fullsize/WT1-Antibody-6F-H2-Immunohistochemistry-Paraffin-NB110-60011-img0006.jpg)
![Western Blot: WT1 Antibody (6F-H2) [NB110-60011] - Whole cell protein from human K562, HEK293 and kidney tissue was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-WT1 in block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/WT1-Antibody-6F-H2-Western-Blot-NB110-60011-img0005.jpg)
![Immunohistochemistry: WT1 Antibody (6F-H2) [NB110-60011] - Analysis of Wilms Tumor 1 in human renal cancer using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/WT1-Antibody-6F-H2-Immunohistochemistry-NB110-60011-img0003.jpg)
![Simple Western: WT1 Antibody (6F-H2) [NB110-60011] - Lane view shows a specific band for WT1 in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/WT1-Antibody-6F-H2-Simple-Western-NB110-60011-img0004.jpg)
![Western Blot: WT1 Antibody (6F-H2) - BSA Free [NB110-60011] - Hypomethylation of Intron 1 CpG island results in WT1 expression. (C) Cell lysates from K562, U937, & HL60 cells treated with CoCl2, 5-azacytidine, or untreated controls analyzed by WB using an antibody against WT1. Histone H3 used as a loading control. Size of molecular weight markers is in kDa. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0119837), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-60011_mouse-monoclonal-wt1-antibody-6f-h2-310202415304296.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (WT1/857 + 6F-H2) [NBP2-44607] - Formalin-fixed, paraffin-embedded human Mesothelioma stained with WT1 Antibody (WT1/857 + 6F-H2).](http://images.novusbio.com/fullsize/WT1-Antibody-WT1-857-+-6F-H2-Immunohistochemistry-Paraffin-NBP2-44607-img0006.jpg "Immunohistochemistry-Paraffin: WT1 Antibody (WT1/857 + 6F-H2) [NBP2-44607] - Formalin-fixed, paraffin-embedded human Mesothelioma stained with WT1 Antibody (WT1/857 + 6F-H2).")
![Immunohistochemistry-Paraffin: WT1 Antibody (WT1/857 + 6F-H2) [NBP2-44607] - Formalin-fixed, paraffin-embedded Rat kidney stained with WT1 monoclonal antibody (WT1/857+6F-H2).](http://images.novusbio.com/fullsize/nbp2-44607_mouse-monoclonal-wt1-antibody-wt1-857-6f-h2-1772024100320.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (WT1/857 + 6F-H2) [NBP2-44607] - Formalin-fixed, paraffin-embedded human Fetal kidney stained with WT1 Monoclonal Antibody (WT1/857+6F-H2).](http://images.novusbio.com/fullsize/nbp2-44607_mouse-monoclonal-wt1-antibody-wt1-857-6f-h2-177202495943.jpg)
![Western Blot: WT1 Antibody (SC06-41) [NBP2-67587] - analysis of Wilms Tumor Protein on K562 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/WT1-Antibody-SC06-41-Western-Blot-NBP2-67587-img0008.jpg "Western Blot: WT1 Antibody (SC06-41) [NBP2-67587] - analysis of Wilms Tumor Protein on K562 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: WT1 Antibody (SC06-41) [NBP2-67587] - Staining Wilms Tumor Protein in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/WT1-Antibody-SC06-41-Immunocytochemistry-Immunofluorescence-NBP2-67587-img0006.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (SC06-41) [NBP2-67587] - Analysis of paraffin-embedded human testis tissue with Rabbit anti-WT1 Tumor Protein antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/WT1-Antibody-SC06-41-Immunohistochemistry-Paraffin-NBP2-67587-img0010.jpg)
![Flow Cytometry: WT1 Antibody (SC06-41) [NBP2-67587] - Analysis of K562 cells with Wilms Tumor Protein antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody](http://images.novusbio.com/fullsize/WT1-Antibody-SC06-41-Flow-Cytometry-NBP2-67587-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: WT1 Antibody (SC06-41) [NBP2-67587] - Staining Wilms Tumor Protein in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/WT1-Antibody-SC06-41-Immunocytochemistry-Immunofluorescence-NBP2-67587-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: WT1 Antibody (SC06-41) [NBP2-67587] - Staining Wilms Tumor Protein in LO2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/WT1-Antibody-SC06-41-Immunocytochemistry-Immunofluorescence-NBP2-67587-img0005.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (SC06-41) [NBP2-67587] - analysis of paraffin-embedded mouse kidney tissue using anti-Wilms Tumor Protein antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/WT1-Antibody-SC06-41-Immunohistochemistry-Paraffin-NBP2-67587-img0007.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (SC06-41) [NBP2-67587] - Analysis of paraffin-embedded human fallopian tube tissue with Rabbit anti-WT1 Tumor Protein antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/WT1-Antibody-SC06-41-Immunohistochemistry-Paraffin-NBP2-67587-img0009.jpg)
![Western Blot: WT1 Antibody (CL13170) [NBP3-18547] - Analysis in human cell line HEK 293 and human cell line SK-MEL-30.](http://images.novusbio.com/fullsize/WT1-Antibody-CL13170-Western-Blot-NBP3-18547-img0020.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: WT1 Antibody (CL13170) [NBP3-18547] - Analysis in human cell line HEK 293 and human cell line SK-MEL-30.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: WT1 Antibody (CL13170) [NBP3-18547] - Staining of CACO-2 cells using the Anti-WT1 monoclonal antibody, showing specific staining in nucleoplasm in green. Microtubule probes are visualized in red (where available).](http://images.novusbio.com/fullsize/WT1-Antibody-CL13170-Immunocytochemistry-Immunofluorescence-NBP3-18547-img0002.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (CL13170) [NBP3-18547] - Staining of human prostate shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/WT1-Antibody-CL13170-Immunohistochemistry-Paraffin-NBP3-18547-img0018.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (CL13170) [NBP3-18547] - Analysis in human testis and prostate tissues using NBP3-18547 antibody. Corresponding WT1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/WT1-Antibody-CL13170-Immunohistochemistry-Paraffin-NBP3-18547-img0004.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (CL13170) [NBP3-18547] - Staining of human ovarian cancer (endometrioid carcinoma) shows strong nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/WT1-Antibody-CL13170-Immunohistochemistry-Paraffin-NBP3-18547-img0012.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (CL13170) [NBP3-18547] - Staining of human ovarian cancer (high grade serous carcinoma) shows strong nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/WT1-Antibody-CL13170-Immunohistochemistry-Paraffin-NBP3-18547-img0013.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (CL13170) [NBP3-18547] - Staining of human ovarian cancer (papillary carcinoma) shows strong nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/WT1-Antibody-CL13170-Immunohistochemistry-Paraffin-NBP3-18547-img0014.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (CL13170) [NBP3-18547] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/WT1-Antibody-CL13170-Immunohistochemistry-Paraffin-NBP3-18547-img0015.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (CL13170) [NBP3-18547] - Staining of human fallopian tube shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/WT1-Antibody-CL13170-Immunohistochemistry-Paraffin-NBP3-18547-img0016.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (CL13170) [NBP3-18547] - Staining of human kidney shows strong nuclear positivity in cells in glomeruli.](http://images.novusbio.com/fullsize/WT1-Antibody-CL13170-Immunohistochemistry-Paraffin-NBP3-18547-img0017.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (CL13165) [NBP3-18564] - Analysis in human fallopian tube and lymph node tissues using NBP3-18564 antibody. Corresponding WT1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/WT1-Antibody-CL13165-Immunohistochemistry-Paraffin-NBP3-18564-img0002.jpg "Immunohistochemistry-Paraffin: WT1 Antibody (CL13165) [NBP3-18564] - Analysis in human fallopian tube and lymph node tissues using NBP3-18564 antibody. Corresponding WT1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: WT1 Antibody (CL13165) [NBP3-18564] - Analysis in human cell line HEK 293 and human cell line SK-MEL-30.](http://images.novusbio.com/fullsize/WT1-Antibody-CL13165-Western-Blot-NBP3-18564-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: WT1 Antibody (CL13165) [NBP3-18564] - Staining of CACO-2 cells using the Anti-WT1 monoclonal antibody, showing specific staining in nucleoplasm in green. Microtubule probes are visualized in red (where available).](http://images.novusbio.com/fullsize/WT1-Antibody-CL13165-Immunocytochemistry-Immunofluorescence-NBP3-18564-img0001.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (CL13165) [NBP3-18564] - Staining of human lymph node shows no positivity in germinal center cells as expected.](http://images.novusbio.com/fullsize/WT1-Antibody-CL13165-Immunohistochemistry-Paraffin-NBP3-18564-img0009.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (CL13165) [NBP3-18564] - Staining of human ovarian cancer (high grade serous carcinoma) shows strong nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/WT1-Antibody-CL13165-Immunohistochemistry-Paraffin-NBP3-18564-img0003.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (CL13165) [NBP3-18564] - Staining of human ovarian cancer (endometrioid carcinoma) shows strong nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/WT1-Antibody-CL13165-Immunohistochemistry-Paraffin-NBP3-18564-img0004.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (CL13165) [NBP3-18564] - Staining of human ovarian cancer (papillary carcinoma) shows strong nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/WT1-Antibody-CL13165-Immunohistochemistry-Paraffin-NBP3-18564-img0005.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (CL13165) [NBP3-18564] - Staining of human fallopian tube shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/WT1-Antibody-CL13165-Immunohistochemistry-Paraffin-NBP3-18564-img0006.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (CL13165) [NBP3-18564] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/WT1-Antibody-CL13165-Immunohistochemistry-Paraffin-NBP3-18564-img0007.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (CL13165) [NBP3-18564] - Staining of human kidney shows strong nuclear positivity in cells in glomeruli.](http://images.novusbio.com/fullsize/WT1-Antibody-CL13165-Immunohistochemistry-Paraffin-NBP3-18564-img0008.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody [NBP1-31314] - Mouse testis. Wilms Tumor 1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/WT1-Antibody-Immunohistochemistry-Paraffin-NBP1-31314-img0008.jpg "Immunohistochemistry-Paraffin: WT1 Antibody [NBP1-31314] - Mouse testis. Wilms Tumor 1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Immunohistochemistry-Paraffin: WT1 Antibody [NBP1-31314] - Mouse liver. Wilms Tumor 1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/WT1-Antibody-Immunohistochemistry-Paraffin-NBP1-31314-img0005.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody [NBP1-31314] - Rat testis. Wilms Tumor 1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/WT1-Antibody-Immunohistochemistry-Paraffin-NBP1-31314-img0006.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody [NBP1-31314] - Rat testis. Wilms Tumor 1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/WT1-Antibody-Immunohistochemistry-Paraffin-NBP1-31314-img0007.jpg)
![Western Blot: WT1 Antibody [NBP1-31314] - Various tissue extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with wt1a + wt1b antibody (NBP1-31314) diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp1-31314_rabbit-polyclonal-wt1-antibody-91020242014636.jpg)
![Western Blot: WT1 Antibody (6L9X6) [NBP3-16135] - Western blot analysis of extracts of Rat testis, using WT1 antibody (NBP3-16135) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/WT1-Antibody-6L9X6-Western-Blot-NBP3-16135-img0006.jpg "Western Blot: WT1 Antibody (6L9X6) [NBP3-16135] - Western blot analysis of extracts of Rat testis, using WT1 antibody (NBP3-16135) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Western Blot: WT1 Antibody (6L9X6) [NBP3-16135] -Analysis of lysates from Mouse testis, using WT1 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-16135_rabbit-wt1-mab-6l9x6-western-blot-288202411746..jpg)
![Western Blot: WT1 Antibody (6L9X6) [NBP3-16135] - Analysis of various lysates using WT1 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-16135_rabbit-wt1-mab-6l9x6-western-blot-2882024111043..jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (6L9X6) [NBP3-16135] - Human Ovarian Serous Carcinoma tissue using WT1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16135_rabbit-wt1-mab-6l9x6-immunohistochemistry-paraffin-2882024111235..jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (6L9X6) [NBP3-16135] - Human kidney tissue using WT1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16135_rabbit-wt1-mab-6l9x6-immunohistochemistry-paraffin-2882024111344..jpg)
![Immunocytochemistry: WT1 Antibody (960522) [MAB5729] - WT-1 was detected in immersion fixed K-562 cell line using MAB5729 at 8 ug/ml for 3 hours at room temperature. Cells were stained using NL007 (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplam & nuclei.](http://images.novusbio.com/fullsize/WT1-Antibody-960522-Immunocytochemistry-MAB5729-img0002.jpg "Immunocytochemistry: WT1 Antibody (960522) [MAB5729] - WT-1 was detected in immersion fixed K-562 cell line using MAB5729 at 8 ug/ml for 3 hours at room temperature. Cells were stained using NL007 (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplam & nuclei.")
![Flow Cytometry: WT1 Antibody (960522) [MAB5729] - An intracellular stain was performed on THP-1 cells with WT1 [960522] Antibody MAB5729AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/WT1-Antibody-960522-Flow-Cytometry-MAB5729-img0003.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (rWT1/857) [NBP2-53181] - Formalin-fixed, paraffin-embedded Rat Testis stained with WT1 Antibody (rWT1/857).](http://images.novusbio.com/fullsize/WT1-Antibody-rWT1-857-Immunohistochemistry-Paraffin-NBP2-53181-img0004.jpg "Immunohistochemistry-Paraffin: WT1 Antibody (rWT1/857) [NBP2-53181] - Formalin-fixed, paraffin-embedded Rat Testis stained with WT1 Antibody (rWT1/857).")
![Immunohistochemistry-Paraffin: WT1 Antibody (rWT1/857) [NBP2-53181] - Formalin-fixed, paraffin-embedded human Testis stained with WT1 Antibody (rWT1/857).](http://images.novusbio.com/fullsize/WT1-Antibody-rWT1-857-Immunohistochemistry-Paraffin-NBP2-53181-img0002.jpg)
![Protein Array: WT1 Antibody (rWT1/857) [NBP2-53181] - Analysis of Protein Array containing more than 19,000 full-length human proteins using WT1 Antibody (rWT1/857).Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/WT1-Antibody-rWT1-857-Protein-Array-NBP2-53181-img0005.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (rWT1/857) [NBP2-53181] - Formalin-fixed, paraffin-embedded human Mesothelioma stained with WT1 Antibody (rWT1/857).](http://images.novusbio.com/fullsize/nbp2-53181_mouse-monoclonal-wt1-antibody-rwt1-857-1772024938132.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (WT1/857) [NBP2-44606] - Formalin-fixed, paraffin-embedded Rat Testis stained with WT1 Antibody (WT1/857).](http://images.novusbio.com/fullsize/WT1-Antibody-WT1-857-Immunohistochemistry-Paraffin-NBP2-44606-img0008.jpg "Immunohistochemistry-Paraffin: WT1 Antibody (WT1/857) [NBP2-44606] - Formalin-fixed, paraffin-embedded Rat Testis stained with WT1 Antibody (WT1/857).")
![Immunohistochemistry-Paraffin: WT1 Antibody (WT1/857) [NBP2-44606] - Formalin-fixed, paraffin-embedded human Mesothelioma stained with WT1 Antibody (WT1/857).](http://images.novusbio.com/fullsize/WT1-Antibody-WT1-857-Immunohistochemistry-Paraffin-NBP2-44606-img0006.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (WT1/857) [NBP2-44606] - Formalin-fixed, paraffin-embedded Human Testis stained with WT1 Antibody (WT1/857).](http://images.novusbio.com/fullsize/WT1-Antibody-WT1-857-Immunohistochemistry-Paraffin-NBP2-44606-img0007.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (WT1/857) [NBP2-44606] - Formalin-fixed, paraffin-embedded Rat kidney stained with WT1 Monoclonal Antibody (WT1/857)](http://images.novusbio.com/fullsize/nbp2-44606_mouse-monoclonal-wt1-antibody-wt1-857-17720241044626.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (WT1/857 + 6F-H2) - Azide and BSA Free [NBP2-47858] - Formalin-fixed, paraffin-embedded human Fetal kidney stained with WT1 Monoclonal Antibody (WT1/857+6F-H2).](http://images.novusbio.com/fullsize/WT1-Antibody-WT1-857-+-6F-H2-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47858-img0004.jpg "Immunohistochemistry-Paraffin: WT1 Antibody (WT1/857 + 6F-H2) - Azide and BSA Free [NBP2-47858] - Formalin-fixed, paraffin-embedded human Fetal kidney stained with WT1 Monoclonal Antibody (WT1/857+6F-H2).")
![Immunohistochemistry-Paraffin: WT1 Antibody (WT1/857 + 6F-H2) - Azide and BSA Free [NBP2-47858] - Formalin-fixed, paraffin-embedded Rat kidney stained with WT1 monoclonal antibody (WT1/857+6F-H2).](http://images.novusbio.com/fullsize/WT1-Antibody-WT1-857-+-6F-H2-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47858-img0001.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (WT1/857 + 6F-H2) - Azide and BSA Free [NBP2-47858] - Formalin-fixed, paraffin-embedded human Mesothelioma stained with WT1 Antibody (WT1/857 + 6F-H2).](http://images.novusbio.com/fullsize/WT1-Antibody-WT1-857-+-6F-H2-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47858-img0003.jpg)