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WT1 Antibody (6L9X6)

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Western Blot: WT1 Antibody (6L9X6) [NBP3-16135] - Western blot analysis of extracts of Rat testis, using WT1 antibody (NBP3-16135) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Western Blot: WT1 Antibody (6L9X6) [NBP3-16135] - Western blot analysis of extracts of Rat testis, using WT1 antibody (NBP3-16135) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 ...read more
Western Blot: WT1 Antibody (6L9X6) [NBP3-16135] -Analysis of lysates from Mouse testis, using WT1 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: ...read more
Western Blot: WT1 Antibody (6L9X6) [NBP3-16135] - Analysis of various lysates using WT1 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per ...read more
Immunohistochemistry-Paraffin: WT1 Antibody (6L9X6) [NBP3-16135] - Human Ovarian Serous Carcinoma tissue using WT1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M ...read more
Immunohistochemistry-Paraffin: WT1 Antibody (6L9X6) [NBP3-16135] - Human kidney tissue using WT1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, IHC
Clone
6L9X6
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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WT1 Antibody (6L9X6) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 60-250 of human WT1 (P19544). PPPPPPPPPHSFIKQEPSWGGAEPHEEQCLSAFTVHFSGQFTGTAGACRYGPFGPPPPSQASSGQARMFPNAPYLPSCLESQPAIRNQGYSTVTFDGTPSYGHTPSHHAAQFPNHSFKHEDPMGQQGSLGEQQYSVPPPVYGCHTPTDSCTGSQALLLRTPYSSDNLYQMTSQLECMTWNQMNLGATLKGV
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
WT1
Purity
Affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500 - 1:1000
Theoretical MW
49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for WT1 Antibody (6L9X6)

  • AWT1
  • GUD
  • NPHS4GUD
  • WAGR
  • Wilms tumor 1
  • Wilms tumor protein
  • WIT-2
  • WT1
  • WT33

Background

Wilms' tumor protein (WT1) is a transcription factor which regulates the expression of numerous target genes, including EPO. This protein plays an essential role in the normal development of the urogenital system, including cellular development and cell survival, and it has both tumor suppressor and oncogenic roles in tumor formation.

Defects in the WT1 protein can lead to Wilms tumor, Frasier syndrome, Denys-Drash syndrome, nephrotic syndrome type 4, and Meacham syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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WT1 Antibody
(6L9X6)
NBP3-16135
WT1 Antibody (6L9X6)
Species: Hu, Mu, Rt
Applications: WB, ELISA, IHC

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Product General Protocols

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Bioinformatics

Gene Symbol WT1