![Western Blot: Nucleophosmin Antibody [NB110-61646] - Detection of mutant Nucleophosmin in OCI-AML3 lysates.](http://images.novusbio.com/fullsize/Nucleophosmin-Antibody-Western-Blot-NB110-61646-img0012.jpg "Western Blot: Nucleophosmin Antibody [NB110-61646] - Detection of mutant Nucleophosmin in OCI-AML3 lysates.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Immunogen | A synthetic peptide made to a C-terminal region of human mutant Nucleophosmin (within residues 250-294). [Accession# AAW67752] |
| Localization | Nucleophosmin typically resides in the nucleus. However, in patients with acute myelogenous leukemia (AML) it mutates and localizes to the cytoplasm. |
| Specificity | This antibody is specific for the mutant form of Nucleophosmin. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | NPM1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | In WB a band can be seen at ~37 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Theoretical MW | 37 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.4) |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||||||
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reviewed by:
Verified Customer |
ICC | Human | 03/16/2018 |
Summary
Comments
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reviewed by:
Verified Customer |
WB | Human | 01/18/2018 |
Summary
Comments
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Secondary Antibodies |
Isotype Controls |
Research Areas for NPM1 Antibody (NB110-61646)Find related products by research area.
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| Verified Customer 03/16/2018 |
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| Application: | ICC | |
| Species: | Human |
| Verified Customer 01/18/2018 |
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| Application: | WB | |
| Species: | Human |
| Gene Symbol | NPM1 |
![Immunocytochemistry/Immunofluorescence: Nucleophosmin Antibody [NB110-61646] - Nucleophosmin localization by immunofluorescence in HL-60 cells (negative control).](http://images.novusbio.com/fullsize/Nucleophosmin-Antibody-Immunocytochemistry-Immunofluorescence-NB110-61646-img0011.jpg "Immunocytochemistry/Immunofluorescence: Nucleophosmin Antibody [NB110-61646] - Nucleophosmin localization by immunofluorescence in HL-60 cells (negative control).")
![Immunocytochemistry/Immunofluorescence: Nucleophosmin Antibody [NB110-61646] - Nucleophosmin localization by immunofluorescence in OCI-AML3 cells.](http://images.novusbio.com/fullsize/Nucleophosmin-Antibody-Immunocytochemistry-Immunofluorescence-NB110-61646-img0010.jpg "Immunocytochemistry/Immunofluorescence: Nucleophosmin Antibody [NB110-61646] - Nucleophosmin localization by immunofluorescence in OCI-AML3 cells.")
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![Western Blot: Nucleophosmin Overexpression Lysate (Adult Normal) [NBL1-13748] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Nucleophosmin.](https://images.novusbio.com/images/Nucleophosmin-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-13748-img0002.jpg "Western Blot: Nucleophosmin Overexpression Lysate (Adult Normal) [NBL1-13748] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Nucleophosmin.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
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