NPM1 Antibody:
Western Blot
1. Run 50 ug of cytoplasmic fraction into each lane of an 12% SDS-PAGE.
2. Block the membrane.
3. Dilute the primary anti-mNPM1 (NB110-61646) in 1% NFDM and incubate for 2 hours at room temperature.
4. Wash the membrane.
5. Dilute the sedondary anti-rabbit IgG, conjugated to HRP in 1% NFDM and incubate for 1 hour at room temperature.
6. Develop the blot.
