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WT1 Antibody (960522)

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Immunocytochemistry: WT1 Antibody (960522) [MAB5729] - WT-1 was detected in immersion fixed K-562 cell line using MAB5729 at 8 ug/ml for 3 hours at room temperature. Cells were stained using NL007 (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplam & nuclei.
Immunocytochemistry: WT1 Antibody (960522) [MAB5729] - WT-1 was detected in immersion fixed K-562 cell line using MAB5729 at 8 ug/ml for 3 hours at room temperature. Cells were stained using NL007 (red) and ...read more
Flow Cytometry: WT1 Antibody (960522) [MAB5729] - An intracellular stain was performed on THP-1 cells with WT1 [960522] Antibody MAB5729AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, CyTOF-ready, ICC/IF
Clone
960522
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
MAB5729AF350
Alexa Fluor 405
MAB5729AF405
Alexa Fluor 488
MAB5729AF488
Alexa Fluor 532
MAB5729AF532
Alexa Fluor 594
MAB5729AF594
Alexa Fluor 647
MAB5729AF647
Alexa Fluor 700
MAB5729AF700
Alexa Fluor 750
MAB5729AF750
Allophycocyanin
MAB5729APC
DyLight 350
MAB5729UV
DyLight 405
MAB5729V
DyLight 488
MAB5729G
DyLight 550
MAB5729R
DyLight 594
MAB5729DL594
DyLight 650
MAB5729C
DyLight 680
MAB5729FR
DyLight 755
MAB5729IR
FITC
MAB5729F
Janelia Fluor 549
MAB5729JF549
Janelia Fluor 646
MAB5729JF646
PE
MAB5729PE
PerCP
MAB5729PCP
mFluor Violet 450 SE
MAB5729MFV450
mFluor Violet 500 SE
MAB5729MFV500
mFluor Violet 610 SE
MAB5729MFV610
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WT1 Antibody (960522) Summary

Immunogen
E. coli-derived recombinant human WT1
Met127-Gly249
Accession # P19544
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
WT1
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry
  • Immunocytochemistry
Application Notes
This antibody is Cytof ready.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Notes

0

Alternate Names for WT1 Antibody (960522)

  • AWT1
  • GUD
  • NPHS4GUD
  • WAGR
  • Wilms tumor 1
  • Wilms tumor protein
  • WIT-2
  • WT1
  • WT33

Background

Wilms' tumor protein (WT1) is a transcription factor which regulates the expression of numerous target genes, including EPO. This protein plays an essential role in the normal development of the urogenital system, including cellular development and cell survival, and it has both tumor suppressor and oncogenic roles in tumor formation.

Defects in the WT1 protein can lead to Wilms tumor, Frasier syndrome, Denys-Drash syndrome, nephrotic syndrome type 4, and Meacham syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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WT1 Antibody
(960522)
MAB5729
WT1 Antibody (960522)
Species: Hu
Applications: Flow, CyTOF-ready, ICC/IF

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol WT1