Home » WT1 » WT1 Antibodies » WT1 Antibody (WT1/857 + 6F-H2) [Biotin]
Novus Biologicals products are now on bio-techne.com

WT1 Antibody (WT1/857 + 6F-H2) [Biotin]

 Submit a Review

Images

 
There are currently no images for WT1 Antibody (NBP2-47858B).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications IHC, CyTOF-ready
Clone
WT1/857 + 6F-H2
Clonality
Monoclonal
Host
Mouse
Conjugate
Biotin

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-47858AF350
Alexa Fluor 405
NBP2-47858AF405
Alexa Fluor 488
NBP2-47858AF488
Alexa Fluor 532
NBP2-47858AF532
Alexa Fluor 594
NBP2-47858AF594
Alexa Fluor 647
NBP2-47858AF647
Alexa Fluor 700
NBP2-47858AF700
Alexa Fluor 750
NBP2-47858AF750
DyLight 350
NBP2-47858UV
DyLight 405
NBP2-47858V
DyLight 488
NBP2-47858G
DyLight 550
NBP2-47858R
DyLight 594
NBP2-47858DL594
DyLight 650
NBP2-47858C
DyLight 680
NBP2-47858FR
DyLight 755
NBP2-47858IR
FITC
NBP2-47858F
HRP
NBP2-47858H
Janelia Fluor 549
NBP2-47858JF549
Janelia Fluor 646
NBP2-47858JF646
Unconjugated
NBP2-44607-0.1mg
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-44607-0.1mg
Azide and BSA Free
NBP2-47858-0.1mg
IHC-Prediluted
NBP2-48223
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

WT1 Antibody (WT1/857 + 6F-H2) [Biotin] Summary

Immunogen
Recombinant full length human WT1 protein (WT1/857); Recombinant fragment aa1-181 of human WT1 (6F-H2) (Uniprot: P19544)
Localization
Nuclear and Cytoplasmic
Marker
Wilm's Tumor & Mesothelial Marker
Specificity
Recognizes a 47-55kDa-tumor suppressor protein, identified as Wilms tumor and mesothelioma. WT1 protein has been identified in proliferative mesothelial cells, malignant mesothelioma, ovarian carcinoma, gonadoblastoma, nephroblastoma, and desmoplastic small round cell tumor. Lung adenocarcinomas rarely stain positive with this antibody. WT1 protein expression in mesothelial cells has become a reliable marker for the diagnosis of mesotheliomas.
Isotype
IgG1 Kappa/IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
WT1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:33631230).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Alternate Names for WT1 Antibody (WT1/857 + 6F-H2) [Biotin]

  • AWT1
  • GUD
  • NPHS4GUD
  • WAGR
  • Wilms tumor 1
  • Wilms tumor protein
  • WIT-2
  • WT1
  • WT33

Background

Wilms' tumor protein (WT1) is a transcription factor which regulates the expression of numerous target genes, including EPO. This protein plays an essential role in the normal development of the urogenital system, including cellular development and cell survival, and it has both tumor suppressor and oncogenic roles in tumor formation.

Defects in the WT1 protein can lead to Wilms tumor, Frasier syndrome, Denys-Drash syndrome, nephrotic syndrome type 4, and Meacham syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...


SF-1/NR5A1/Steroidogen ...
NBP1-52823
Immunohistochemistry-Paraffin SF-1/NR5A1/Steroidogenic Factor 1 Antibody
Western Blot SF-1/NR5A1/Steroidogenic Factor 1 Antibody
(4 Publications)
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, Simple Western, WB
p53 Antibody (PAb
240) - BSA Free
NB200-103
Flow Cytometry p53 Antibody (PAb 240) - BSA Free
Flow Cytometry p53 Antibody (PAb 240) - BSA Free
Flow (Intracellular) p53 Antibody (PAb 240) - BSA Free
(3 Reviews)
(42 Publications)
Species: Hu, Mu, Rt, Xp(-), Ye
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
EGR1 Antibody
[Unconjugated]
AF2818
Immunocytochemistry EGR1 Antibody [Unconjugated]
(13 Publications)
Species: Hu
Applications: ICC, WB
EWSR1 Antibody
NB200-182
Immunohistochemistry-Paraffin EWSR1 Antibody
Immunoprecipitation EWSR1 Antibody
Western Blot EWSR1 Antibody
(1 Publication)
Species: Hu, Mu
Applications: IHC, IHC-P, IP, Simple Western, WB
Pax2 Antibody
[Unconjugated]
AF3364
Immunocytochemistry Pax2 Antibody [Unconjugated]
Western Blot Pax2 Antibody [Unconjugated]
Immunohistochemistry Pax2 Antibody [Unconjugated]
(28 Publications)
Species: Hu
Applications: ICC, IHC, WB
Flt-3/Flk-2/CD135
Antibody (66903) [Unc ...
MAB812
Flow Cytometry Flt-3/Flk-2/CD135 Antibody (66903) [Unconjugated]
Flow Cytometry Flt-3/Flk-2/CD135 Antibody (66903) [Unconjugated]
(4 Publications)
Species: Hu
Applications: CyTOF-ready, Flow
IGF-II/IGF2
[Unconjugated]
292-G2
SDS-PAGE IGF-II/IGF2 [Unconjugated]
Bioactivity IGF-II/IGF2 [Unconjugated]
(48 Publications)
Species: Hu
Applications: BA
Nephrin Antibody
[Unconjugated]
AF3159
Immunohistochemistry Nephrin Antibody [Unconjugated]
Immunohistochemistry Nephrin Antibody [Unconjugated]
Immunocytochemistry/ Immunofluorescence Nephrin Antibody [Unconjugated]
(58 Publications)
Species: Mu
Applications: IHC
DAX1/NR0B1
Antibody (H7431)
PP-H7431-00
DAX1/NR0B1 Antibody (H7431)
(2 Publications)
Species: Hu
Applications: WB
SRY Antibody (1G4)
- BSA Free
NBP2-37463
ELISA SRY Antibody (1G4) - BSA Free
Western Blot SRY Antibody (1G4) - BSA Free
Western Blot SRY Antibody (1G4) - BSA Free
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, IHC, IHC-P, WB
SOX9 Antibody
[Unconjugated]
AF3075
Immunocytochemistry SOX9 Antibody [Unconjugated]
Western Blot SOX9 Antibody [Unconjugated]
Immunocytochemistry SOX9 Antibody [Unconjugated]
(2 Reviews)
(154 Publications)
Species: Hu
Applications: ICC, Simple Western, WB
NPM1 Antibody -
BSA Free
NB110-61646
Immunocytochemistry/ Immunofluorescence NPM1 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence NPM1 Antibody - BSA Free
Western Blot NPM1 Antibody - BSA Free
(2 Reviews)
(4 Publications)
Species: Hu, Mu
Applications: Flow, ICC/IF, IHC, IHC-P, IP, WB
Podocin/NPHS2
Antibody (JB51-33)
NBP2-75624
Flow Cytometry Podocin/NPHS2 Antibody (JB51-33)
Immunohistochemistry-Paraffin Podocin/NPHS2 Antibody (JB51-33)
Immunohistochemistry-Paraffin Podocin/NPHS2 Antibody (JB51-33)
(6 Publications)
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, MI, WB
Vimentin Antibody
NB300-223
Immunocytochemistry/ Immunofluorescence Vimentin Antibody
Immunohistochemistry-Frozen Vimentin Antibody
Immunocytochemistry/ Immunofluorescence Vimentin Antibody
(6 Reviews)
(70 Publications)
Species: Bv, Ca, Ch, Eq, Hu, Mu, Po, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
Desmin Antibody
[Unconjugated]
AF3844
Immunohistochemistry Desmin Antibody [Unconjugated]
Immunohistochemistry Desmin Antibody [Unconjugated]
Immunocytochemistry/ Immunofluorescence Desmin Antibody [Unconjugated]
(2 Reviews)
(34 Publications)
Species: Hu, Mu
Applications: IHC
CD34 Antibody
[Unconjugated]
AF4117
Immunohistochemistry CD34 Antibody [Unconjugated]
(3 Reviews)
(37 Publications)
Species: Rt
Applications: IHC, WB
Exosome component
1 Antibody (OTI1H9)
NBP2-45545
Immunohistochemistry Exosome component 1 Antibody (OTI1H9)
Immunohistochemistry Exosome component 1 Antibody (OTI1H9)
Immunohistochemistry Exosome component 1 Antibody (OTI1H9)
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
Pax6 Antibody
[Unconjugated]
AF8150
Simple Western Pax6 Antibody [Unconjugated]
Immunocytochemistry Pax6 Antibody [Unconjugated]
Western Blot Pax6 Antibody [Unconjugated]
(16 Publications)
Species: Hu
Applications: ICC, ICFlow, Simple Western, WB
c-Abl Antibody
[Unconjugated]
AF5414
Western Blot c-Abl Antibody [Unconjugated]
Simple Western c-Abl Antibody [Unconjugated]
(1 Publication)
Species: Hu
Applications: Simple Western, WB
WT1 Antibody
(WT1/857 + 6F-H2) [Bi
NBP2-47858B
WT1 Antibody (WT1/857 + 6F-H2) [Biotin]
Species: Hu, Mu, Rt
Applications: IHC, CyTOF-ready

Publications for WT1 Antibody (NBP2-47858B) (0)

There are no publications for WT1 Antibody (NBP2-47858B).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for WT1 Antibody (NBP2-47858B) (0)

There are no reviews for WT1 Antibody (NBP2-47858B). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for WT1 Antibody (NBP2-47858B) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional WT1 Products

Research Areas for WT1 Antibody (NBP2-47858B)

Find related products by research area.

Blogs on WT1.

Check out the latest blog posts on WT1.
Routine WT1 Antibody Screen Uncovers an Exciting New Cancer-Cleaning Enzyme
Enzyme antibodies are widely used in both apoptosis and cancer studies, as disruption of the proteins regulating apoptosis is known to lead to formation of tumor cells in certain cancers. We at Novus Biologicals are continually growing our enzyme anti...  Read full blog post.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

SOX9 was detected in immersion fixed BG01V human embryonic stem cells differentiated into early proximal lung progenitor cells using Goat Anti-Human SOX9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3075) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
SOX9 Antibody [Unconjugated]
AF3075

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our WT1 Antibody (WT1/857 + 6F-H2) [Biotin] and receive a gift card or discount.


Bioinformatics

Gene Symbol WT1