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WT1 Antibody (WT1/857) - IHC-Prediluted

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Immunohistochemistry-Paraffin: WT1 Antibody (WT1/857) - IHC-Prediluted [NBP2-48216] - Formalin-fixed, paraffin-embedded Rat Testis stained with Wilm's Tumor Mouse Monoclonal Antibody (WT1/857).
Immunohistochemistry-Paraffin: WT1 Antibody (WT1/857) - IHC-Prediluted [NBP2-48216] - Formalin-fixed, paraffin-embedded Rat Testis stained with Wilm's Tumor Mouse Monoclonal Antibody (WT1/857).
Immunohistochemistry-Paraffin: WT1 Antibody (WT1/857) - IHC-Prediluted [NBP2-48216] - Formalin-fixed, paraffin-embedded human Mesothelioma stained with Wilm's Tumor Mouse Monoclonal Antibody (WT1/857).
Immunohistochemistry-Paraffin: WT1 Antibody (WT1/857) - IHC-Prediluted [NBP2-48216] - Formalin-fixed, paraffin-embedded HumanTestis stained with Wilm's Tumor Mouse Monoclonal Antibody (WT1/857)

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications IHC
Clone
WT1/857
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
IHC-Prediluted

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-47931AF350
Alexa Fluor 405
NBP2-47931AF405
Alexa Fluor 488
NBP2-47931AF488
Alexa Fluor 532
NBP2-47931AF532
Alexa Fluor 594
NBP2-47931AF594
Alexa Fluor 647
NBP2-47931AF647
Alexa Fluor 700
NBP2-47931AF700
Alexa Fluor 750
NBP2-47931AF750
Biotin
NBP2-47931B
CoraFluor 1
NBP2-47931CL1
DyLight 350
NBP2-47931UV
DyLight 405
NBP2-47931V
DyLight 488
NBP2-47931G
DyLight 550
NBP2-47931R
DyLight 594
NBP2-47931DL594
DyLight 650
NBP2-47931C
DyLight 680
NBP2-47931FR
DyLight 755
NBP2-47931IR
FITC
NBP2-47931F
HRP
NBP2-47931H
Janelia Fluor 525
NBP2-47931JF525
Janelia Fluor 549
NBP2-47931JF549
Janelia Fluor 646
NBP2-47931JF646
Janelia Fluor 669
NBP2-47931JF669
Unconjugated
NBP2-44606-0.1mg
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-44606-0.1mg
Azide and BSA Free
NBP2-47931-0.1mg
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WT1 Antibody (WT1/857) - IHC-Prediluted Summary

Description
The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.
Immunogen
Recombinant full-length human WT1 protein (Uniprot: P19544)
Localization
Nuclear and Cytoplasmic
Marker
Wilm's Tumor & Mesothelial Marker
Specificity
Recognizes a 47-55kDa-tumor suppressor protein, identified as Wilms tumor and mesothelioma. WT1 protein has been identified in proliferative mesothelial cells, malignant mesothelioma, ovarian carcinoma, gonadoblastoma, nephroblastoma, and desmoplastic small round cell tumor. Lung adenocarcinomas rarely stain positive with this antibody. WT1 protein expression in mesothelial cells has become a reliable marker for the diagnosis of mesotheliomas.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
WT1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Reactivity Notes

Weakly reacts with Mouse

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Alternate Names for WT1 Antibody (WT1/857) - IHC-Prediluted

  • AWT1
  • GUD
  • NPHS4GUD
  • WAGR
  • Wilms tumor 1
  • Wilms tumor protein
  • WIT-2
  • WT1
  • WT33

Background

Wilms' tumor protein (WT1) is a transcription factor which regulates the expression of numerous target genes, including EPO. This protein plays an essential role in the normal development of the urogenital system, including cellular development and cell survival, and it has both tumor suppressor and oncogenic roles in tumor formation.

Defects in the WT1 protein can lead to Wilms tumor, Frasier syndrome, Denys-Drash syndrome, nephrotic syndrome type 4, and Meacham syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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WT1 Antibody
(WT1/857) - IHC-Predi
NBP2-48216
WT1 Antibody (WT1/857) - IHC-Prediluted
Species: Hu, Rt
Applications: IHC

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Bioinformatics

Gene Symbol WT1