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VEGFR2/KDR/Flk-1 Antibody (89106) [Phycoerythrin]

Images

 
HUVEC human umbilical vein endothelial cells were stained with Mouse Anti-Human VEGF R2/KDR/Flk-1 PE-conjugated Mono-clonal Antibody (Catalog # FAB357P, filled histogram) or isotype control antibody (Catalog # IC002P, ...read more
Circulating EPC number in healthy controls and diabetes. a and b: Circulating EPC numbers were determined by flow cytometry for the co-expression of CD34 and VEGFR2 (b). Peripheral blood MNCs incubated with IgG isotype ...read more
Phenotypic characterization of cultured PACs. A) PACs were characterized for surface marker expression by flow cytometry. In the side scatted (SSC) versus forward scatter (FSC) morphologic plot, lymphocytic cells ...read more
Flow cytometry-derived EPC definitions.(A) Cells isolated from whole blood by density gradient separation were gated by forward and side scatter to select mononuclear cells by excluding erythrocytes, granulocytes and ...read more
Circulating EPC number in healthy controls and diabetes. a and b: Circulating EPC numbers were determined by flow cytometry for the co-expression of CD34 and VEGFR2 (b). Peripheral blood MNCs incubated with IgG isotype ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
89106
Clonality
Monoclonal
Host
Mouse
Conjugate
Phycoerythrin

Order Details

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VEGFR2/KDR/Flk-1 Antibody (89106) [Phycoerythrin] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human VEGF R2/KDR/Flk-1
Ala20-Glu764
Accession # P35968
Specificity
Detects human VEGF R2/KDR/Flk-1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) VEGF R1, rhVEGF R3 or recombinant mouse VEGF R2 is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
KDR
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 10 uL/10^6 cells
Reviewed Applications
Read 3 Reviews rated 4.7
using
FAB357P in the following application:

Publications
Read Publications using
FAB357P in the following applications:

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VEGFR2/KDR/Flk-1 Antibody (89106) [Phycoerythrin]

  • CD309 antigen
  • CD309
  • EC 2.7.10
  • EC 2.7.10.1
  • Fetal liver kinase 1
  • fetal liver kinase-1
  • Flk1
  • Flk-1
  • FLK1tyrosine kinase growth factor receptor
  • KDR
  • kinase insert domain receptor (a type III receptor tyrosine kinase)
  • Kinase insert domain receptor
  • KRD1
  • Ly73
  • Protein-tyrosine kinase receptor flk-1
  • soluble VEGFR2
  • vascular endothelial growth factor receptor 2
  • VEGF R2
  • VEGFR
  • VEGFR2
  • VEGFR-2

Background

VEGF R2 (KDR/Flk-1), VEGF R1 (Flt-1) and VEGF R3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGF R1, 2, and 3 is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis. Mature VEGF R2 is composed of a 745 aa extracellular domain, a 25 aa transmembrane domain and a 567 aa cytoplasmic domain. In contrast to VEGF R1 which binds both PlGF and VEGF with high affinity, VEGF R2 binds VEGF but not PlGF with high affinity. The recombinant soluble VEGF R2/Fc chimera binds VEGF with high affinity and is a potent VEGF antagonist.

  1. Ferra, N. and R. Davis-Smyth (1997) Endocrine Reviews 18:4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for VEGFR2/KDR/Flk-1 Antibody (FAB357P)(122)

We have publications tested in 4 confirmed species: Human, Mouse, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Primate - Macaca nemestrina (Southern Pig-tailed Macaque).

We have publications tested in 6 applications: Cell Selection, FLOW, Flow Cytometry, ICC, Neutralization, Western Blot.


Filter By Application
Cell Selection
(1)
FLOW
(1)
Flow Cytometry
(96)
ICC
(3)
Neutralization
(1)
Western Blot
(1)
All Applications
Filter By Species
Human
(98)
Mouse
(2)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
(1)
Primate - Macaca nemestrina (Southern Pig-tailed Macaque)
(1)
All Species
Showing Publications 1 - 10 of 122. Show All 122 Publications.
Publications using FAB357P Applications Species
Bisson, JA;Gordillo, M;Kumar, R;de Silva, N;Yang, E;Banks, KM;Shi, ZD;Lee, K;Yang, D;Chung, WK;Huangfu, D;Evans, T; GATA6 regulates WNT and BMP programs to pattern precardiac mesoderm during the earliest stages of human cardiogenesis bioRxiv : the preprint server for biology 2024-07-11 [PMID: 39026742] (Flow Cytometry, Human) Flow Cytometry Human
Raval, AN;Schmuck, EG;Roy, S;Saito, Y;Zhou, T;Conklin, J;Hacker, TA;Koonce, C;Boyer, M;Stack, K;Hebron, E;Nagle, SK;Hsieh, PCH;Kamp, TJ; Human iPSC-derived Committed Cardiac Progenitors Generate Cardiac Tissue Grafts in a Swine Ischemic Cardiomyopathy Model without Triggering Ventricular Arrhythmias bioRxiv : the preprint server for biology 2024-02-17 [PMID: 38405927] (Flow Cytometry, Human) Flow Cytometry Human
Wilken, MB;Maguire, JA;Dungan, LV;Gagne, A;Osorio-Quintero, C;Waxman, EA;Chou, ST;Gadue, P;French, DL;Thom, CS; Generation of a human Tropomyosin 1 knockout iPSC line Stem cell research 2023-06-28 [PMID: 37422949] (Flow Cytometry, Human) Flow Cytometry Human
Bansal, A;Singh, A;Nag, TC;Sharma, D;Garg, B;Bhatla, N;Choudhury, SD;Ramakrishnan, L; Augmenting the Angiogenic Profile and Functionality of Cord Blood Endothelial Colony-Forming Cells by Indirect Priming with Bone-Marrow-Derived Mesenchymal Stromal Cells Biomedicines 2023-05-05 [PMID: 37239042] (Flow Cytometry, Human) Flow Cytometry Human
Wilken, MB;Maguire, JA;Dungan, LV;Gagne, A;Osorio-Quintero, C;Waxman, EA;Chou, ST;Gadue, P;French, DL;Thom, CS; Generation of a human Tropomyosin 1 knockout iPSC line bioRxiv : the preprint server for biology 2023-05-04 [PMID: 37205377] (ICC, Human) ICC Human
APT Del Rio, JO Frade-Guan, S Ospina-Pri, BKL Duarte, MB Bertolo, MC Ozelo, Z Sachetto Impaired repair properties of endothelial colony-forming cells in patients with granulomatosis with polyangiitis Journal of Cellular and Molecular Medicine, 2022-09-02;0(0):. 2022-09-02 [PMID: 36052734] (Flow Cytometry, Human) Flow Cytometry Human
S Simoncini, S Toupance, C Labat, S Gautier, C Dumoulin, L Arnaud, MG Stathopoul, S Visvikis-S, PM Rossi, A Benetos, F Dignat-Geo, F Sabatier Functional Impairment of Endothelial Colony Forming Cells (ECFC) in Patients with Severe Atherosclerotic Cardiovascular Disease (ASCVD) International Journal of Molecular Sciences, 2022-08-11;23(16):. 2022-08-11 [PMID: 36012229] (Human) Human
Antonietta Maio, Maria Ida Maiorino, Miriam Longo, Lorenzo Scappaticcio, Vlenia Pernice, Paolo Cirillo et al. Change in Circulating Levels of Endothelial Progenitor Cells and Sexual Function in Women With Type 1 Diabetes The Journal of Clinical Endocrinology & Metabolism 8/18/2022 [PMID: 35583559]
J Philip Cre, SA Luff, H Yu, CM Sturgeon CD1d expression demarcates CDX4+ hemogenic mesoderm with definitive hematopoietic potential Stem Cell Research, 2022-05-08;62(0):102808. 2022-05-08 [PMID: 35569347] (Flow Cytometry, Human) Flow Cytometry Human
CH Gil, D Chakrabort, CP Vieira, N Prasain, SL Calzi, SD Fortmann, P Hu, K Banno, M Jamal, C Huang, MS Sielski, Y Lin, X Huang, MD Dupont, JL Floyd, R Prasad, ALF Longhini, TJ McGill, HM Chung, MP Murphy, DN Kotton, ME Boulton, MC Yoder, MB Grant Specific mesoderm subset derived from human pluripotent stem cells ameliorates microvascular pathology in type 2 diabetic mice Science Advances, 2022-03-04;8(9):eabm5559. 2022-03-04 [PMID: 35245116] (Flow Cytometry, Human) Flow Cytometry Human
Show All 122 Publications.

Reviews for VEGFR2/KDR/Flk-1 Antibody (FAB357P) (3) 4.73

Average Rating: 4.7
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Mouse.

Reviews using FAB357P:
Filter by Applications
Flow
(3)
All Applications
Filter by Species
Human
(2)
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
Flow Mouse 01/23/2015
View

Summary

ApplicationFlow Cytometry
Sample TestedSee PMID: 18410528
SpeciesMouse
  5
reviewed by:
Verified Customer
Flow Human 01/23/2015
View

Summary

ApplicationFlow Cytometry
Sample TestedSee PMID: 24449499
SpeciesHuman
  4
reviewed by:
Verified Customer
Flow Human 01/23/2015
View

Summary

ApplicationFlow Cytometry
Sample TestedSee PMID: 22024722
SpeciesHuman

Product General Protocols

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FAQs for VEGFR2/KDR/Flk-1 Antibody (FAB357P) (0)

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Recent Reviews

4.7
5
2
4
1
3
0
2
0
1
0

 
Verified Customer
01/23/2015
Application: Flow
Species: Mouse

 
Verified Customer
01/23/2015
Application: Flow
Species: Human

 
Verified Customer
01/23/2015
Application: Flow
Species: Human

Bioinformatics

Gene Symbol KDR
Uniprot