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Neuropilin-1 Antibody [Unconjugated]

Images

 
Western blot shows 25 ng of Recombinant Mouse Neuropilin-1 (Catalog # 5994-N1), Recombinant Rat Neuropilin-1 Fc Chimera (Catalog # 566-N1) and Recombinant Human Neuropilin-1 (Catalog # 3870-N1). PVDF Membrane was probed ...read more
bEnd.3 mouse endothelioma cell line was stained with Goat Anti-Rat Neuropilin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF566, filled histogram) or isotype control antibody (Catalog # AB‑108‑C, ...read more
Neuropilin-1 was detected in immersion fixed frozen sections of embryonic rat spinal cord (15 d.p.c.) using 5 µg/mL Goat Anti-Rat Neuropilin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF566) overnight ...read more
Neuropilin‑1 was detected in perfusion fixed frozen sections of rat spinal cord using Goat Anti-Rat Neuropilin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF566) at 15 µg/mL overnight at 4 °C. ...read more
Neuropilin‑1 was detected in immersion fixed paraffin-embedded sections of rat brain (hippocampus) using Goat Anti-Mouse/Rat Neuropilin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF566) at 3 ...read more
Neuropilin‑1 was detected in immersion fixed paraffin-embedded sections of rat brain (thalamus) using Goat Anti-Mouse/Rat Neuropilin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF566) at 3 ...read more
Nrp1 influences Smad2/3 activation.(a–c) Representative confocal images of wt sprouts from EBs immunolabelled for pSmad2. The outline of the wt sprouts is indicated with a dashed line using the endogenous DsRED ...read more
Expression of candidate genes. (A) qPCR analysis of relative mRNA levels in total RNA from isolated glomeruli of Foxc2fl/fl; Pod‐Cre+, Foxc2fl/+; Pod‐Cre+ and Foxc2fl/+ mice. Expression levels were normalized ...read more
Genetic Strategies: Nrp1 influences Smad2/3 activation.(a–c) Representative confocal images of wt sprouts from EBs immunolabelled for pSmad2. The outline of the wt sprouts is indicated with a dashed line using ...read more

Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications WB, Flow, IHC, B/N, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
     

Genetic Strategies

   

Order Details

View Available Conjugates
View Available Formulations
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Neuropilin-1 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant rat Neuropilin‑1
Phe22-Ala810 (Lys811Arg), Ser829-Asp854
Accession # Q9QWJ9
Specificity
Detects mouse and rat Neuropilin-1 in direct ELISAs and Western blots. In direct ELISAs less than 1% cross-reactivity with recombinant rat Neuropilin-2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
NRP1
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Blockade of Receptor-ligand Interaction
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 3-15 ug/mL
  • Western Blot 0.1 ug/mL
Reviewed Applications
Read 10 Reviews rated 4.2
using
AF566 in the following applications:

Publications
Read Publications using
AF566 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product or the use of this product is covered by U.S. Patents owned by The Regents of the University of California. This product is for research use only and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for such purposes, contact The Regents of the University of California.

U.S. Patent # 6,054,293, 6,623,738, and other U.S. and international patents pending.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Neuropilin-1 Antibody [Unconjugated]

  • BDCA4
  • BDCA-4
  • CD304 antigen
  • CD304
  • DKFZp686A03134
  • DKFZp781F1414
  • neuropilin 1
  • Neuropilin1
  • Neuropilin-1
  • NP1
  • NRP
  • NRP1
  • transmembrane receptor
  • Vascular endothelial cell growth factor 165 receptor
  • VEGF165R

Background

Neuropilin-1 is a type I transmembrane protein that is expressed in the developing nervous system and by endothelial and tumor cells. Neuropilin-1 binds members of the class III secreted semaphorin subfamily as well as some isoforms of VEGF family proteins. The amino acid sequence of rat Neuropilin-1 extracellular domain is 98% and 93% identical to that of mouse and human Neuropilin-1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Neuropilin-1 Antibody (AF566)(108)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Chicken, Transgenic Mouse.

We have publications tested in 12 applications: Cell Culture, Flow Cytometry, Functional Assay, ICC, IHC, IHC-Fr, IHC-P, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Neutralization, Western Blot.


Filter By Application
Cell Culture
(1)
Flow Cytometry
(7)
Functional Assay
(1)
ICC
(3)
IHC
(23)
IHC-Fr
(7)
IHC-P
(2)
Immunocytochemistry
(2)
Immunohistochemistry
(2)
Immunoprecipitation
(2)
Neutralization
(7)
Western Blot
(12)
All Applications
Filter By Species
Human
(5)
Mouse
(50)
Rat
(5)
Chicken
(1)
Transgenic Mouse
(2)
All Species
Showing Publications 1 - 10 of 108. Show All 108 Publications.
Publications using AF566 Applications Species
Joyce, JD;Moore, GA;Goswami, P;Harrell, TL;Taylor, TM;Hawks, SA;Green, JC;Jia, M;Irwin, MD;Leslie, E;Duggal, NK;Thompson, CK;Bertke, AS; SARS-CoV-2 Rapidly Infects Peripheral Sensory and Autonomic Neurons, Contributing to Central Nervous System Neuroinvasion before Viremia International journal of molecular sciences 2024-07-28 [PMID: 39125815] (Western Blot, Immunocytochemistry, Mouse, Transgenic Mouse) Western Blot, Immunocytochemistry Mouse, Transgenic Mouse
Hoard, TM;Liu, K;Cadigan, KM;Giger, RJ;Allen, BL; Semaphorin Receptors Antagonize Wnt Signaling Through Beta-Catenin Degradation bioRxiv : the preprint server for biology 2024-05-29 [PMID: 38854152] (Western Blot, Immunocytochemistry, Mouse) Western Blot, Immunocytochemistry Mouse
Barnkob, MB;Michaels, YS;André, V;Macklin, PS;Gileadi, U;Valvo, S;Rei, M;Kulicke, C;Chen, JL;Jain, V;Woodcock, VK;Colin-York, H;Hadjinicolaou, AV;Kong, Y;Mayya, V;Mazet, JM;Mead, GJ;Bull, JA;Rijal, P;Pugh, CW;Townsend, AR;Gérard, A;Olsen, LR;Fritzsche, M;Fulga, TA;Dustin, ML;Jones, EY;Cerundolo, V; Semmaphorin 3?A causes immune suppression by inducing cytoskeletal paralysis in tumour-specific CD8+ T cells Nature communications 2024-04-12 [PMID: 38609390] (Neutralization, Mouse) Neutralization Mouse
Hani, T;Fujita, K;Kudo, T;Taya, Y;Sato, K;Soeno, Y; Tissue-Targeted Transcriptomics Reveals SEMA3D Control of Hypoglossal Nerve Projection to Mouse Tongue Primordia Acta histochemica et cytochemica 2024-02-29 [PMID: 38463205] (Immunohistochemistry, Mouse) Immunohistochemistry Mouse
Sauve F, Nampoothiri S, Clarke SA et al. Long-COVID cognitive impairments and reproductive hormone deficits in men may stem from GnRH neuronal death EBioMedicine 2023-09-13 [PMID: 37713808]
Altounian, M;Bellon, A;Mann, F; Neuronal miR-17-5p contributes to interhemispheric cortical connectivity defects induced by prenatal alcohol exposure Cell reports 2023-08-22 [PMID: 37610874] (Immunohistochemistry, Mouse) Immunohistochemistry Mouse
Suzuki, K;Kunisada, Y;Miyamura, N;Eikawa, S;Hurtado de Mendoza, T;Mose, ES;Lu, C;Kuroda, Y;Ruoslahti, E;Lowy, AM;Sugahara, KN; Tumor-resident regulatory T cells in pancreatic cancer express the ?v?5 integrin as a targetable activation marker bioRxiv : the preprint server for biology 2023-05-24 [PMID: 37292693] (IHC, Mouse) IHC Mouse
Emy Bosseboeuf, Anissa Chikh, Ahmed Bey Chaker, Tom P. Mitchell, Dhilakshani Vignaraja, Ridhi Rajendrakumar et al. Neuropilin-1 interacts with VE-cadherin and TGFBR2 to stabilize adherens junctions and prevent activation of endothelium under flow Science Signaling 2023-05-23 [PMID: 37220183]
Teppei Matsuda, Kazuki Misato, Shigeyuki Tamiya, Yasuhiro Akeda, Ikuhiko Nakase, Etsushi Kuroda et al. Efficient antigen delivery by dendritic cell-targeting peptide via nucleolin confers superior vaccine effects in mice iScience 2022-11-18 [PMID: 36304121]
Luis F. Martins, Ilaria Brambilla, Alessia Motta, Stefano de Pretis, Ganesh Parameshwar Bhat, Aurora Badaloni et al. Motor neurons use push-pull signals to direct vascular remodeling critical for their connectivity Neuron 2022-12-21 [PMID: 36240771]
Show All 108 Publications.

Reviews for Neuropilin-1 Antibody (AF566) (10) 4.210

Average Rating: 4.2
(Based on 10 reviews)
We have 10 reviews tested in 4 species: Human, Mouse, Rat, Other.

Reviews using AF566:
Filter by Applications
IF
(6)
IHC-Fr
(1)
WB
(3)
All Applications
Filter by Species
Human
(1)
Mouse
(7)
Rat
(1)
Other
(1)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
IF Other 01/08/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 22927827
SpeciesOther
  4
reviewed by:
Verified Customer
IHC-Fr Mouse 01/08/2015
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedSee PMID 24034253
SpeciesMouse
  4
reviewed by:
Verified Customer
IF Mouse 01/08/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 23406903
SpeciesMouse
  4
reviewed by:
Verified Customer
IF Mouse 01/08/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 24021649
SpeciesMouse
  4
reviewed by:
Verified Customer
IF Mouse 01/08/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 23602477
SpeciesMouse
  5
reviewed by:
Verified Customer
IF Mouse 12/18/2014
View

Summary

ApplicationImmunofluorescence
Sample TestedPrimary mouse hippocampal neurons
SpeciesMouse
  4
reviewed by:
Verified Customer
IF Mouse 12/18/2014
View

Summary

ApplicationImmunofluorescence
Sample TestedHippocampal neurons and astrocytes
SpeciesMouse
  4
reviewed by:
Verified Customer
WB Rat 12/18/2014
View

Summary

ApplicationWestern Blot
Sample TestedRN22 cells
SpeciesRat
  4
reviewed by:
Verified Customer
WB Mouse 12/18/2014
View

Summary

ApplicationWestern Blot
Sample TestedMouse primary cultures
SpeciesMouse
  4
reviewed by:
Verified Customer
WB Human 12/18/2014
View

Summary

ApplicationWestern Blot
Sample Testeda number of human cell lines
SpeciesHuman

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Neuropilin-1 Antibody (AF566). (Showing 1 - 2 of 2 FAQs).

  1. If this product is used in an application or species as a part of a customer review, will that validate this product in the application/species?
    • If any of our primary antibodes are used in an untested application or species and it is shown to work through images from customer reviews or through publications, this validates the application/species for this product. Please check out our Innovator's Reward Program if you decide to test an antibody with a species or application that is not currently listed.
  2. What research areas can Neuropilin-1 antibodies be used in?
    • Neuropilin-1 products can be applied in the following research areas: Adaptive Immunity, Angiogenesis, Breast Cancer, Cancer, Cardiovascular Biology, Neuroscience, and Virology Bacteria and Parasites.
mFluor Violet Conjugated Antibodies

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Recent Reviews

4.2
5
2
4
8
3
0
2
0
1
0

 
Verified Customer
01/08/2015
Application: IF
Species: Other

 
Verified Customer
01/08/2015
Application: IHC-Fr
Species: Mouse

 
Verified Customer
01/08/2015
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol NRP1
Uniprot