Home » VEGFR2/KDR/Flk-1 » VEGFR2/KDR/Flk-1 Antibodies » VEGFR2/KDR/Flk-1 Antibody (89106) [Fluorescein]

VEGFR2/KDR/Flk-1 Antibody (89106) [Fluorescein]

 (11 Publications)
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HUVEC human umbilical vein endothelial cells were stained with Mouse Anti-Human VEGF R2/KDR/Flk-1 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB357F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.
HUVEC human umbilical vein endothelial cells were stained with Mouse Anti-Human VEGF R2/KDR/Flk-1 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB357F, filled histogram) or isotype control antibody (Catalog # ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
89106
Clonality
Monoclonal
Host
Mouse
Conjugate
Fluorescein

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB3572U-100UG
Alexa Fluor 405
FAB3572V-100UG
Alexa Fluor 488
FAB3572G-100UG
Alexa Fluor 532
FAB3572X
Alexa Fluor 594
FAB3572T-100UG
Alexa Fluor 647
FAB3572R-100UG
Alexa Fluor 700
FAB357N-100
Alexa Fluor 700
FAB357N-025
Alexa Fluor 750
FAB3572S-100UG
Allophycocyanin
FAB357A-025
Allophycocyanin
FAB357A-100
Allophycocyanin/Cy7
FAB3572APCCY7
Biotin
FAB3572B
DyLight 350
FAB3572D
DyLight 405
FAB3572E
DyLight 488
FAB3572K
DyLight 550
FAB3572L
DyLight 594
FAB3572M
DyLight 650
FAB3572W
DyLight 680
FAB3572Y
DyLight 755
FAB3572Z
Fluorescein
FAB357F-025
HRP
FAB3572H
Janelia Fluor 549
FAB3572I
Janelia Fluor 646
FAB3572J
PE/Atto594
FAB3572PEATT594
PE/Cy5.5
FAB3572PECY55
PE/Cy7
FAB3572PECY7
PerCP (Peridinin-chlorophyll Protein Complex)
FAB357C-100
PerCP (Peridinin-chlorophyll Protein Complex)
FAB357C-025
Phycoerythrin
FAB357P-025
Phycoerythrin
FAB357P-100
Unconjugated
MAB3572-100
mFluor Violet 450 SE
FAB3572MFV450
mFluor Violet 500 SE
FAB3572MFV500
mFluor Violet 610 SE
FAB3572MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB3572-100
Carrier Free
FAB357P-025
Carrier Free
FAB3572R-100UG
Carrier Free
FAB3572S-100UG
Carrier Free
FAB3572T-100UG
Carrier Free
FAB3572U-100UG
Carrier Free
FAB3572V-100UG
Carrier Free
MAB3572-500
Carrier Free
MAB3572-100
Carrier Free
FAB357P-100
Carrier Free
MAB3572-SP
Carrier Free
FAB357A-025
Carrier Free
FAB357A-100
Carrier Free
FAB357N-025
Carrier Free
FAB357N-100
Carrier Free
FAB357F-025
Carrier Free
FAB357C-025
Carrier Free
FAB357C-100
Carrier Free
FAB3572G-100UG
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VEGFR2/KDR/Flk-1 Antibody (89106) [Fluorescein] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human VEGF R2/KDR/Flk-1
Ala20-Glu764
Accession # P35968
Specificity
Detects human VEGF R2/KDR/Flk-1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) VEGF R1, rhVEGF R3 or recombinant mouse VEGF R2 is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
KDR
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 10 uL/10^6 cells
Publications
Read Publications using
FAB357F in the following applications:

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VEGFR2/KDR/Flk-1 Antibody (89106) [Fluorescein]

  • CD309 antigen
  • CD309
  • EC 2.7.10
  • EC 2.7.10.1
  • Fetal liver kinase 1
  • fetal liver kinase-1
  • Flk1
  • Flk-1
  • FLK1tyrosine kinase growth factor receptor
  • KDR
  • kinase insert domain receptor (a type III receptor tyrosine kinase)
  • Kinase insert domain receptor
  • KRD1
  • Ly73
  • Protein-tyrosine kinase receptor flk-1
  • soluble VEGFR2
  • vascular endothelial growth factor receptor 2
  • VEGF R2
  • VEGFR
  • VEGFR2
  • VEGFR-2

Background

VEGF R2 (KDR/Flk-1), VEGF R1 (Flt-1) and VEGF R3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGF R1, 2, and 3 is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis. Mature VEGF R2 is composed of a 745 aa extracellular domain, a 25 aa transmembrane domain and a 567 aa cytoplasmic domain. In contrast to VEGF R1 which binds both PlGF and VEGF with high affinity, VEGF R2 binds VEGF but not PlGF with high affinity. The recombinant soluble VEGF R2/Fc chimera binds VEGF with high affinity and is a potent VEGF antagonist.

  1. Ferra, N. and R. Davis-Smyth (1997) Endocrine Reviews 18:4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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VEGFR2/KDR/Flk-1
Antibody (89106) [Flu ...
FAB357F
VEGFR2/KDR/Flk-1 Antibody (89106) [Fluorescein]
Species: Hu
Applications: Flow

Publications for VEGFR2/KDR/Flk-1 Antibody (FAB357F)(11)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Flow Cytometry.


Filter By Application
Flow Cytometry
(8)
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Human
(7)
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Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using FAB357F Applications Species
N Alexandru, E Andrei, F Safciuc, E Dragan, AM Balahura, E Badila, A Georgescu Intravenous Administration of Allogenic Cell-Derived Microvesicles of Healthy Origins Defend Against Atherosclerotic Cardiovascular Disease Development by a Direct Action on Endothelial Progenitor Cells Cells, 2020-02-12;9(2):. 2020-02-12 [PMID: 32059493] (Flow Cytometry, Human) Flow Cytometry Human
HJ Wen, GF Liu, LZ Xiao, YG Wu Involvement of endothelial nitric oxide synthase pathway in IGF?1 protects endothelial progenitor cells against injury from oxidized LDLs Mol Med Rep, 2018-11-08;0(0):. 11/8/2018 [PMID: 30431094]
R Di Liddo, T Bertalot, A Borean, I Pirola, A Argentoni, S Schrenk, C Cenzi, S Capelli, MT Conconi, PP Parnigotto Leucocyte and Platelet-rich Fibrin: a carrier of autologous multipotent cells for regenerative medicine J. Cell. Mol. Med., 2018-01-05;0(0):. 2018-01-05 [PMID: 29314633] (Flow Cytometry, Human) Flow Cytometry Human
S Kapur, AP Silverman, AZ Ye, N Papo, D Jindal, MS Blumenkran, JR Cochran Engineered ligand-based VEGFR antagonists with increased receptor binding affinity more effectively inhibit angiogenesis Bioeng Transl Med, 2017-02-17;2(1):81-91. 2017-02-17 [PMID: 28516164] (Flow Cytometry) Flow Cytometry
Mamun AA, Hayashi H, Sakima M, Sato M. Adenosine triphosphate is a critical determinant for VEGFR signal during hypoxia. Am. J. Physiol., Cell Physiol. 11/9/2016 [PMID: 27834196]
Innovative Flow Cytometry Allows Accurate Identification of Rare Circulating Cells Involved in Endothelial Dysfunction PLoS ONE, 2016-08-25;11(8):e0160153. 2016-08-25 [PMID: 27560136] (Flow Cytometry, Human) Flow Cytometry Human
De Biasi S, Cerri S, Bianchini E, Gibellini L, Persiani E, Montanari G, Luppi F, Carbonelli C, Zucchi L, Bocchino M, Zamparelli A, Vancheri C, Sgalla G, Richeldi L, Cossarizza A Levels of circulating endothelial cells are low in idiopathic pulmonary fibrosis and are further reduced by anti-fibrotic treatments. BMC Med, 2015-11-09;13(0):277. 2015-11-09 [PMID: 26552487] (Flow Cytometry, Human) Flow Cytometry Human
Sarah Jones, Helen Bischof, Ingrid Lang, Gernot Desoye, Sue L. Greenwood, Edward D. Johnstone et al. Dysregulated flow‐mediated vasodilatation in the human placenta in fetal growth restriction The Journal of Physiology 7/15/2015 [PMID: 25920377]
Maeng Y, Kwon J, Kim E, Kwon Y Heterochromatin Protein 1 Alpha (HP1alpha: CBX5) is a Key Regulator in Differentiation of Endothelial Progenitor Cells to Endothelial Cells. Stem Cells, 2015-05-01;33(5):1512-22. 2015-05-01 [PMID: 25588582] (Flow Cytometry, Human) Flow Cytometry Human
Kishuku M, Nishioka Y, Abe S, Kishi J, Ogino H, Aono Y, Azuma M, Kinoshita K, Batmunkh R, Rentsenhand B, Makino H, Ranjan P, Minakuchi K, Sone S Expression of soluble vascular endothelial growth factor receptor-1 in human monocyte-derived mature dendritic cells contributes to their antiangiogenic property. J. Immunol., 2009-12-15;183(12):8176-85. 2009-12-15 [PMID: 20007583] (Flow Cytometry, Human) Flow Cytometry Human
Show All 11 Publications.

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Bioinformatics

Gene Symbol KDR
Uniprot