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TrkA [p Tyr785] Antibody

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Western blot shows lysates of PC-12 rat adrenal pheochromocytoma cell line untreated (-) or treated (+) with the indicated concentrations of Recombinant Rat beta -NGF (Catalog # 556-NG) for 5 minutes. PVDF membrane was ...read more

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

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TrkA [p Tyr785] Antibody Summary

Immunogen
Phosphopeptide containing human TrkA Y785 site
Modification
p Tyr785
Specificity
Detects rat TrkA when phosphorylated at Y785 in Western blots. Reactivity with human Phospho-TrkA is expected due to 100% homology with the immunogen.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NTRK1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
AF5479 in the following application:

Publications
Read Publications using
AF5479 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TrkA [p Tyr785] Antibody

  • DKFZp781I14186
  • EC 2.7.10
  • EC 2.7.10.1
  • MTChigh affinity nerve growth factor receptor
  • Neurotrophic tyrosine kinase receptor type 1
  • neurotrophic tyrosine kinase, receptor, type 1
  • NTRK1
  • NTRK-1
  • p140-TrkA
  • TRK1-transforming tyrosine kinase protein
  • TrkA
  • Trk-A
  • TRKAOncogene TRK
  • TRKTRK1
  • tyrosine kinase receptor A

Background

The receptor tyrosine kinase TrkA binds nerve growth factor (NGF) with low affinity, initiating a signaling cascade that mediates neuronal survival and differentiation. Higher affinity binding of NGF requires a complex formed between TrkA and the p75 NGF receptor. Ligand binding induces receptor dimerization and autophosphorylation on multiple tyrosine residues, including Y490, Y751 and Y785. Phosphorylation of TrkA at Y785 creates a binding site for phospholipase C-gamma (PLC-gamma ), which catalyzes the cleavage of substrate PIP2 to generate the second messengers DAG and IP3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TrkA Antibody (AF5479)(2)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: Western Blot.


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Western Blot
(2)
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Human
(1)
Mouse
(1)
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Review for TrkA Antibody (AF5479) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF5479:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot TrkA AF5479
Enlarge
5
reviewed by:
Verified Customer
WB Human 04/04/2020
View

Summary

ApplicationWestern Blot
Sample TestedA2780 ovarian cancer cells,Human prostate cancer cell line (PC3)
SpeciesHuman
LotCCHV011809A

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for TrkA Antibody (AF5479). (Showing 1 - 1 of 1 FAQs).

  1. I am searching (with difficulty) for an antibody against Trka which can be used in flow cytometry. Since I am planning to use it for bead sorting of live cells it needs to be for a part of the receptor which is on the outside of the cell. Please can you let me know if you can help me with this.
    • NBP1-47436, NB100-98815 and NB100-65266 all bind to the extracellular domain of Trka, however, none of them are guaranteed for use in flow cytometry. Our only flow cytometry guaranteed antibodies bind to the intracellular domain. This does not mean one of these antibodies won't work for flow, we just can not guarantee it. If you would be interested in testing this novel application on one of our Trka antibodies, please take a look at our Innovators Reward Program.

Secondary Antibodies

 

Isotype Controls

Additional TrkA Products

Blogs on TrkA.

Successful Transplantation of Friedreich Ataxia Induced Pluripotent Stem Cell (iPSC)-Derived Sensory Neurons in Dorsal Root Ganglia of Adult Rodents
Jamshed Arslan, Pharm D, PhD The dorsal root ganglia (DRG) are a collection of cell bodies of sensory nerves carrying sensory information – including nociception, mechanoreception and proprioception – from periphera...  Read full blog post.

TrkB: Bridging Ontogenesis and Oncogenesis
Tropomyosin receptor kinase B (TrkB) is a member of the Trk receptor tyrosine kinases family consisting of TrkA, TrkB and TrkC. The sequence of these family members is highly conserved. Interaction of brain-derived neurotrophic factor (BDNF) with its ...  Read full blog post.

TrkB: Docking for Neurotrophins and Beyond.
Tropomyosin receptor kinase B (TrkB) is a member of the Trk receptor tyrosine kinases family consisting of TrkA, TrkB and TrkC. The sequence of these family members is highly conserved. TrK's are activated by several neurotrophins, which are small pro...  Read full blog post.

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Recent Reviews

5
5
1
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1
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Verified Customer
04/04/2020
Application: WB
Species: Human

Bioinformatics

Gene Symbol NTRK1