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STAT1 Antibody

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Western Blot: STAT1 Antibody [NB100-56314] - Western blot analysis of STAT1 in HeLa cell lysate using NB100-56314.
Western Blot: STAT1 Antibody [NB100-56314] - Western blot analysis of STAT1 in HeLa cell lysate using NB100-56314.
Immunocytochemistry/ Immunofluorescence: STAT1 Antibody [NB100-56314] - RH30 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated ...read more
Immunohistochemistry-Paraffin: STAT1 Antibody [NB100-56314] - Analysis of a FFPE tissue section of human lymph node using 1:200 dilution of STAT1 antibody. The staining was developed using HRP labeled anti-rabbit ...read more

Product Details

Summary
Reactivity Hu, Mu, Po, Ca, PmSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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STAT1 Antibody Summary

Immunogen
A synthetic peptide sequence corresponding to amino acids between 700 and 750 of human Stat1 was used as immunogen. This sequence is 100% conserved between Stat1 alpha and Stat1 beta.
Specificity
This antibody is able to detect both the alpha form (p91, ~91 kD) and the beta form (p84, ~84 kD) of STAT1.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
STAT1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1-5 ug/ml
  • Immunohistochemistry-Paraffin
  • Western Blot 1-2 ug/ml
Control
HeLa Whole Cell Lysate
Publications
Read Publication using
NB100-56314 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for STAT1 Antibody

  • CANDF7
  • DKFZp686B04100
  • ISGF-3
  • ISGF-3,91kD
  • signal transducer and activator of transcription 1, 91kDa
  • signal transducer and activator of transcription-1
  • STAT1
  • STAT91

Background

STATs (signal transducers and activators of transcription) are a family of cytoplasmic latent transcription factors that are activated to regulate gene expression in response to a large number of extracellular signaling polypeptides including cytokines, interferons, and growth factors. STAT1 is involved in the TNFR1-TRADD signaling complex. In Hela cells STAT1 acts as a TNFR1-signaling molecule to suppress NF-kB activation. It has also been shown that its activation slows growth and promotes apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-56314
STAT1 Antibody
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Applications: WB, ICC/IF, IHC
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Publications for STAT1 Antibody (NB100-56314)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for STAT1 Antibody (NB100-56314) (0)

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Control Lysate(s)

HeLa Whole Cell Lysate

Secondary Antibodies

 

Isotype Controls

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Research Areas for STAT1 Antibody (NB100-56314)

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Blogs on STAT1.

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By Jamshed Arslan, Pharm. D., PhD. What is STAT6?The cellular pathway comprising tyrosine kinase Janus Kinase (JAK) and the transcription factor STAT connect extracellular signals from various cytokines, hormones an...  Read full blog post.
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Signal transducer and activator of transcription 3 (STAT3) is an important oncogenic transcriptional factor that mediates tumor induced immune suppression.  Specifically, STAT3 transmits signals from cytokines and growth factor receptors in the pla...  Read full blog post.
Read our latest blog and use the new citation tool on bio-techne.com

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Bioinformatics

Gene Symbol STAT1
Entrez
Uniprot