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Smad8 Antibody

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price
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Smad8 Antibody Summary

Immunogen
E. coli-derived recombinant human Smad8
Ala158-Ser233
Accession # NP_005896
Specificity
Detects human Smad8 in direct ELISAs and Western blots. In direct ELISAs, approximately 90% cross-reactivity with recombinant (rh) Smad1 is observed, approximately 20% cross-reactivity with rhSmad4 is observed, and less than 1% cross-reactivity with rhSmad5, rhSmad7, and rhDNAK is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SMAD9
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publication using
AF2309 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Smad8 Antibody

  • MADH6;MADH9;Mothers against decapentaplegic homolog;Mothers against decapentaplegic homolog 9;PPH2;SMAD8;SMAD8/9;SMAD8A;SMAD8B;SMAD9
  • Smad8
  • Smad9

Background

Smads are a family of intracellular proteins that transmit transforming growth factor beta (TGF-beta ) superfamily signals from the cell surface to the nucleus. Smad8 was originally described as MADH6 and is also known as Smad9 and MADH9. Upon signaling by some BMP family members, Smad8 is phosphorylated resulting in its association with the common-mediator subunit, Smad4. This heteromeric complex then translocates into the nucleus to exert transcriptional comodulator activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Smad8 Antibody (AF2309)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Western Blot.


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Western Blot
(1)
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Human
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Bioinformatics

Gene Symbol SMAD9
Uniprot