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BMPR-IA/ALK-3 Antibody [Unconjugated]

 (13 Publications)
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Western blot shows lysates of human skeletal muscle tissue. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human BMPR-IA/ALK-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF346) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for BMPR-IA/ALK-3 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of human skeletal muscle tissue. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human BMPR-IA/ALK-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF346) followed by ...read more
BMPR‑IA/ALK‑3 was detected in immersion fixed paraffin-embedded sections of normal human prostate tissue (negative) and human prostate cancer tissue (positive) using Goat Anti-Human ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF346
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF346-SP
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BMPR-IA/ALK-3 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human BMPR-IA/AKL-3
Gln24-Arg152
Accession # P36894
Specificity
Detects human BMPR-IA in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
BMPR1A
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 2 ug/mL
Publications
Read Publications using
AF346 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BMPR-IA/ALK-3 Antibody [Unconjugated]

  • ACVRLK310q23del
  • ALK-3
  • ALK3EC 2.7.11.30
  • BMP type-1A receptor
  • BMPR1A
  • BMPR-1A
  • BMPRIA
  • BMPR-IA
  • bone morphogenetic protein receptor type-1A
  • bone morphogenetic protein receptor, type IA
  • CD292 antigen
  • CD292
  • EC 2.7.11
  • Serine/threonine-protein kinase receptor R5
  • SKR5
  • type II-like kinase 3

Background

Cellular responses to bone morphogenetic proteins (BMPs) have been shown to be mediated by the formation of hetero-oligomeric complexes of the type I and type II serine/threonine kinase receptors. BMP receptor 1A (BMPR-1A), also known as activin receptor-like kinase (ALK)-3, is one of seven known type I serine/threonine kinases that are required for the signal transduction of TGF-beta family cytokines. In contrast to the TGF-beta receptor system in which the type I receptor does not bind TGF-beta  in the absence of the type II receptor, type I receptors involved in BMP signaling (including BMPR-IA, BMPR-IB/ALK-6, and ActR-I/ALK-2) can independently bind the various BMP family proteins in the absence of type II receptors. Recombinant soluble BMPR-IA binds BMP-4 with high-affinity in solution and is a potent BMP-4 antagonist in vitro. BMPR-IA is ubiquitously expressed during embryogenesis. In adult tissues, BMPR-IA mRNA is also widely distributed with the highest expression levels found in skeletal muscle. The extracellular domain of BMPR-IA shares little amino acid sequence identity with the other mammalian ALK type I receptor kinases, but the cysteine residues are conserved. Human and mouse BMPR-IA are highly conserved and share 98% sequence identity.

  1. Kawabata, M. et al. (1998) Cytokine and Growth Factor Reviews 9:49.
  2. Ebendal, T. et al. (1998) J. Neuroscience Research 51:139.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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BMPR-IA/ALK-3
Antibody [Unconjugate
AF346
BMPR-IA/ALK-3 Antibody [Unconjugated]
Species: Hu
Applications: WB, Flow, CyTOF-ready

Publications for BMPR-IA/ALK-3 Antibody (AF346)(13)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 7 applications: Flow Cytometry, Functional Assay, ICC, IHC, IHC-P, Immunoprecipitation, Western Blot.


Filter By Application
Flow Cytometry
(5)
Functional Assay
(1)
ICC
(1)
IHC
(1)
IHC-P
(2)
Immunoprecipitation
(2)
Western Blot
(2)
All Applications
Filter By Species
Human
(7)
Mouse
(4)
All Species
Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using AF346 Applications Species
Ji-Hye Jang, Min-Seong Kim, Ainsley Mike Antao, Won-Jun Jo, Hyung-Joon Kim, Su-Jin Kim et al. Bioactive Lipid O-cyclic phytosphingosine-1-phosphate Promotes Differentiation of Human Embryonic Stem Cells into Cardiomyocytes via ALK3/BMPR Signaling International Journal of Molecular Sciences 6/29/2021 [PMID: 34209900]
Maëva Chauvin, Véronique Garambois, Sylvie Choblet, Pierre-Emmanuel Colombo, Myriam Chentouf, Laurent Gros et al. Anti-Müllerian hormone concentration regulates activin receptor-like kinase-2/3 expression levels with opposing effects on ovarian cancer cell survival International Journal of Oncology 5/17/2021 [PMID: 34013359]
T Voeltzel, M Flores-Vio, F Zylberszte, S Lefort, M Billandon, S Jeanpierre, S Joly, G Fossard, M Milenkov, F Mazurier, A Nehme, A Belhabri, E Paubelle, X Thomas, M Michallet, F Louache, FE Nicolini, C Caron de F, V Maguer-Sat A new signaling cascade linking BMP4, BMPR1A, ?Np73 and NANOG impacts on stem-like human cell properties and patient outcome Cell Death Dis, 2018-09-27;9(10):1011. 2018-09-27 [PMID: 30262802] (Flow Cytometry, Functional Assay, Human) Flow Cytometry, Functional Assay Human
P Toofan, C Busch, H Morrison, S O'Brien, H Jørgensen, M Copland, H Wheadon Chronic myeloid leukaemia cells require the bone morphogenic protein pathway for cell cycle progression and self-renewal Cell Death Dis, 2018-09-11;9(9):927. 2018-09-11 [PMID: 30206237] (Flow Cytometry, Human) Flow Cytometry Human
Martinez V, Sacedon R, Hidalgo L, Valencia J, Fernandez-Sevilla L, Hernandez-Lopez C, Vicente A, Varas A The BMP Pathway Participates in Human Naive CD4+ T Cell Activation and Homeostasis. PLoS ONE, 2015-06-25;10(6):e0131453. 2015-06-25 [PMID: 26110906] (Flow Cytometry, IHC, Human) Flow Cytometry, IHC Human
Cao Y, Slaney C, Bidwell B, Parker B, Johnstone C, Rautela J, Eckhardt B, Anderson R BMP4 inhibits breast cancer metastasis by blocking myeloid-derived suppressor cell activity. Cancer Res, 2014-09-15;74(18):5091-102. 2014-09-15 [PMID: 25224959] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Robson N, Hidalgo L, McAlpine T, Wei H, Martinez V, Entrena A, Melen G, MacDonald A, Phythian-Adams A, Sacedon R, Maraskovsky E, Cebon J, Ramirez M, Vicente A, Varas A Optimal effector functions in human natural killer cells rely upon autocrine bone morphogenetic protein signaling. Cancer Res, 2014-07-18;74(18):5019-31. 2014-07-18 [PMID: 25038228] (Flow Cytometry, Human) Flow Cytometry Human
Fong D, Bisson M, Laberge G, McManus S, Grenier G, Faucheux N, Roux S Bone morphogenetic protein-9 activates Smad and ERK pathways and supports human osteoclast function and survival in vitro. Cell Signal, 2013-01-08;25(4):717-28. 2013-01-08 [PMID: 23313128] (ICC, Western Blot, Human) ICC, Western Blot Human
Lee J, Son MJ, Woolard K, Donin NM, Li A, Cheng CH, Kotliarova S, Kotliarov Y, Walling J, Ahn S, Kim M, Totonchy M, Cusack T, Ene C, Ma H, Su Q, Zenklusen JC, Zhang W, Maric D, Fine HA Epigenetic-mediated dysfunction of the bone morphogenetic protein pathway inhibits differentiation of glioblastoma-initiating cells. Cancer Cell, 2008-01-01;13(1):69-80. 2008-01-01 [PMID: 18167341] (Western Blot, Human) Western Blot Human
Zeisberg M, Yang C, Martino M, Duncan MB, Rieder F, Tanjore H, Kalluri R Fibroblasts derive from hepatocytes in liver fibrosis via epithelial to mesenchymal transition. J. Biol. Chem., 2007-06-11;282(32):23337-47. 2007-06-11 [PMID: 17562716] (IHC-P, Mouse) IHC-P Mouse
Show All 13 Publications.

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Bioinformatics

Gene Symbol BMPR1A
Uniprot