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GDF-5/BMP-14 Antibody [Unconjugated]

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GDF-5 was detected in immersion fixed frozen sections of mouse embyro (12 d.p.c.) using 15 µg/mL Goat Anti-Mouse GDF-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF853) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
GDF-5 was detected in immersion fixed frozen sections of mouse embyro (12 d.p.c.) using 15 µg/mL Goat Anti-Mouse GDF-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF853) overnight at 4 °C. Tissue was ...read more
Western blot shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse GDF‑5/BMP‑14 Antigen Affinity-purified Polyclonal Antibody ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF853
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF853-SP
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GDF-5/BMP-14 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse GDF-5
Ala376-Arg495
Accession # P43027
Specificity
Detects mouse GDF-5 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 25% cross-reactivity with recombinant mouse (rm) GDF-6 is observed, and less than 5% cross-reactivity with rmGDF-1, rmGDF-3, rmGDF‑8, rmGDF‑9, recombinant human GDF-11, and rhGDF-15 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
GDF5
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF853 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GDF-5/BMP-14 Antibody [Unconjugated]

  • BMP14
  • BMP-14
  • Cartilage-derived morphogenetic protein 1
  • cartilage-derived morphogenetic protein-1
  • CDMP-1
  • CDMP1LAP4
  • GDF5
  • GDF-5
  • growth differentiation factor 5
  • growth/differentiation factor 5
  • OS5
  • radotermin
  • SYNS2

Background

Growth Differentiation Factor 5 (GDF-5), also known as cartilage-derived morphogenetic protein 1 (CDMP-1), is a member of the bone morphogenetic protein (BMP) family which belongs to the transforming growth factor  beta (TGF-beta ) superfamily. GDF-5 is synthesized as a large precursor protein that consists of an N-terminal 19 amino acid (aa) signal sequence, a 362 aa pro region, and a 120 aa C-terminal mature peptide. Mature GDF-5 is a homodimeric protein which contains the characteristic seven conserved cysteine residues. GDF-5, GDF-6, and GDF-7, which share 80-86% identity, define a new subgroup within the BMP family. Like other TGF-beta superfamily proteins, GDF-5 is highly conserved across species. At the amino acid sequence level, mature human and mouse GDF-5 are 98% identical. It has been reported that GDF-5 has multiple functions including regulation of myogenesis, regulation of chondrogenesis, bone morphogenesis, and neuron differentiation and survival. GDF-5 response is mediated by the formation of hetero-oligomeric complexes of type I (BMPR-IB) and type II (BMPR-II or Activin R-II) sereine/threonine kinase receptors, and the activation of Smad proteins (Smad 1, 5, and 8).

  1. Storm, E.E. et al. (1994) Nature 368:639.
  2. Nishitoh, H. et al. (1996) J. Biol. Chem. 271:21345.
  3. Francis-West, P.H. et al. (1999) Development 126:1035.
  4. Massague, J. et al. (2000) Genes and Dev. 14:627.
  5. Settle, S.H. Jr. et al. (2003) Dev. Biol. 254:116.
  6. Inada, M. et al. (1996) BBRC 222:317.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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GDF-5/BMP-14
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GDF-5/BMP-14 Antibody [Unconjugated]
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Publications for GDF-5/BMP-14 Antibody (AF853)(6)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 4 applications: Flow Cytometry, IHC, IHC-P, Western Blot.


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Flow Cytometry
(1)
IHC
(2)
IHC-P
(2)
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(1)
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(5)
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Showing Publications 1 - 6 of 6.
Publications using AF853 Applications Species
Murakami, T;Ruengsinpinya, L;Takahata, Y;Nakaminami, Y;Hata, K;Nishimura, R; HOXA10 promotes Gdf5 expression in articular chondrocytes Scientific reports 2023-12-20 [PMID: 38123662] (IHC, Mouse) IHC Mouse
Bethanie Imogen Ayerst GDF5 Mediated Enhancement of Chondrocyte Phenotype is Inhibited by Heparin; Implication for the use of Heparin in the Clinic and in Tissue Engineering Applications Tissue Eng Part A, 2017-01-06;0(0):. 2017-01-06 [PMID: 27899064] (Flow Cytometry, Western Blot, Mouse) Flow Cytometry, Western Blot Mouse
Li T, Longobardi L, Myers T, Temple J, Chandler R, Ozkan H, Contaldo C, Spagnoli A Joint TGF-beta type II receptor-expressing cells: ontogeny and characterization as joint progenitors. Stem Cells Dev, 2013-02-15;22(9):1342-59. 2013-02-15 [PMID: 23231014] (IHC, Mouse) IHC Mouse
Rebekah S. Cosden-Decker, Melissa M. Bickett, Christian Lattermann, James N. MacLeod Structural and functional analysis of intra-articular interzone tissue in axolotl salamanders Osteoarthritis and Cartilage 2012-11-01 [PMID: 22800772]
Zaidi SH, Huang Q, Momen A, Riazi A, Husain M Growth differentiation factor 5 regulates cardiac repair after myocardial infarction. J. Am. Coll. Cardiol., 2010-01-12;55(2):135-43. 2010-01-12 [PMID: 20117381] (IHC-P, Mouse) IHC-P Mouse
Basile P, Dadali T, Jacobson J, Hasslund S, Ulrich-Vinther M, Soballe K, Nishio Y, Drissi MH, Langstein HN, Mitten DJ, O'Keefe RJ, Schwarz EM, Awad HA Freeze-dried tendon allografts as tissue-engineering scaffolds for Gdf5 gene delivery. Mol. Ther., 2008-01-08;16(3):466-73. 2008-01-08 [PMID: 18180771] (IHC-P, Mouse) IHC-P Mouse

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Bioinformatics

Gene Symbol GDF5
Uniprot