Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The ED50 for this effect is typically 0.85-5 µg/mL.
Source
E. coli-derived mouse GDF-6/BMP-13 protein Thr335-Arg454
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.
Applications/Dilutions
Dilutions
Bioactivity
Theoretical MW
13.7 kDa (monomer). Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using 855-G6 in the following applications:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 300 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Recombinant Mouse GDF-6/BMP-13 Protein
BMP13
BMP-13
CDMP2
GDF16
GDF6
GDF-6
growth differentiation factor 6
Growth/differentiation factor 16
growth/differentiation factor 6
KFS
KFS1
KFSL
MCOP4
MCOPCB6
MGC158100
MGC158101
SCDO4
SGM1
Background
Growth Differentiation Factor 6 (GDF-6), also known as bone morphogenetic protein 13 (BMP-13) or cartilage-derived morphogenetic protein 2 (CDMP-2), is a member of the bone morphogenetic protein (BMP) family which belongs to the transforming growth factor beta (TGF-beta ) superfamily. The mature GDF-6 with 120 amino acids is a homodimeric protein containing the characteristic seven conserved cysteine residues. GDF-5, GDF-6 and GDF-7, which share 80-86% identity, define a new subgroup within the BMP family. Like other TGF-beta superfamily proteins, GDF-6 is highly conserved across species. At the amino acid sequence level, human and mouse GDF‑6 are 99% identical. It has been reported that GDF-6 has multiple functions including regulation of myogenesis, regulation of chondrogenesis, bone morphogenesis, and neuron differentiation and survival. GDF-6 response is mediated by the formation of hetero-oligomeric complexes of type I (BMPR-IB) and type II (BMPR-II or Activin R-II) sereine/threonine kinase receptors, and the activation of Smad proteins (Smad 1, 5, and 8).
Storm, E.E. et al. (1994) Nature 368:639.
Nishitoh, H. et al. (1996) J. Biol. Chem. 271:21345.
Francis-West, P.H. et al. (1999) Development 126:1035.
Massague, J. et al. (2000) Genes and Dev. 14:627.
Inada, M. et al. (1996) BBRC, 222:317.
Settle, S.H., Jr. et al. (2003) Dev. Biol. 254:116.
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