Human Follistatin Quantikine ELISA Kit Summary
Background |
The Quantikine Human Follistatin Immunoassay is a 5.5 hour solid-phase ELISA designed to measure human Follistatin (FS288, FS300, and FS315) in follicular fluid, cell culture supernates, serum, and plasma. It contains Sf 21-expressed recombinant human Follistatin and antibodies raised against the recombinant factor. It has been shown to accurately quantitate recombinant human Follis...tatin. Results obtained using natural Follistatin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural Follistatin. |
Specificity |
Natural and recombinant human Follistatin |
Source |
N/A |
Assay Type |
Solid Phase Sandwich ELISA |
Inter-Assay |
|
Intra-Assay |
|
Spike Recovery |
|
Sample Volume |
|
Gene |
FST |
Applications/Dilutions
Dilutions |
|
Application Notes |
Interference observed with 1 or more available related molecules. |
Publications |
|
Packaging, Storage & Formulations
Storage |
Store the unopened product at 2 - 8 °C. Do not use past expiration date. |
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Human Follistatin Quantikine ELISA Kit
Background
Follistatin (FST) is a secreted glycoprotein that binds and neutralizes several TGF-beta superfamily proteins including Activin, BMP-6, BMP-7, and GDF-8/Myostatin. It also binds Angiogenin and regulates hematopoietic stem cell adhesion to Fibronectin. Some Follistatin binding partners will also bind Follistatin-like proteins such as FSL-3. Of three follistatin isoforms, the full-length mature follistatin (FST315) is the most abundant and the sole form in plasma, but has lower binding affinity for both activins and heparins than alternative forms. The acidic tail is missing in the splice variant FST288, which shows highest affinity, and partial in the proteolytically produced FST303, which shows intermediate affinity. Genetic deletion of follistatin in mice, or expression of only the FST288 form, is lethal perinatally due to defects of lung, skin and musculoskeletal system. Expression of only the FST315 isoform allows survival, with defects in vascularization and female fertility.
Customers Who Viewed This Item Also Viewed...
Species: Hu, Mu, Rt
Applications: BA
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, IP, WB
Species: Hu, Mu, Rt
Applications: BA
Species: Hu
Applications: IHC, IHC-P, WB
Species: Hu
Applications: BA, BA
Species: Hu, Mu
Applications: IHC-P, WB
Species: Mu
Applications: ELISA
Species: Hu
Applications: BA
Species: Hu
Applications: ELISA
Species: Hu, Mu, Po, Rt, Sh
Applications: Flow, ICC/IF, IHC, IHC-P, PEP-ELISA, WB
Species: Hu
Applications: ELISA
Species: Hu, Mu, Rt
Applications: BA
Species: Hu
Applications: BA
Species: Hu
Applications: BA
Species: Hu, Mu, Rt, Ze
Applications: ICC/IF, IHC, IHC-P, WB
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ICC, IHC, ICFlow, Simple Western, WB
Species: Hu
Applications: BA
Species: Mu
Applications: BA
Species: Hu
Applications: ELISA
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.
Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
FAQs for Follistatin (DFN00) (0)
Additional Follistatin Products
Blogs on Follistatin