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CHX10 Antibody

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Western Blot: CHX10 Antibody [NBP1-84476] - Analysis in control (vector only transfected HEK293T lysate) and vSX2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: CHX10 Antibody [NBP1-84476] - Staining of human skeletal muscle shows no nuclear positivity in myocytes as expected.
Immunohistochemistry-Paraffin: CHX10 Antibody [NBP1-84476] - Staining of human retina shows strong nuclear positivity in inner nuclear layer.
Immunohistochemistry-Paraffin: CHX10 Antibody [NBP1-84476] - Staining of rat eye shows moderate to strong nuclear positivity in inner nuclear layer.

Product Details

Summary
Reactivity Hu, Rt, MuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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CHX10 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LGLNKEPPSSHPRAALDGLAPGHLLAARSVLSPAGVGGMGLLGPGGLPGFYTQPTFLEVLSDPQSVHLQPLGRASGPLDTSQTASSDSEDVSSSDRKMSKSALNQTKKRKKRRHRTIFTSYQLEELEKAFNEAH
Marker
pan-Bipolar Cell Marker
Predicted Species
Mouse (99%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
VSX2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
CHX10 Protein (NBP1-84476PEP)
Publications
Read Publications using
NBP1-84476 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for CHX10 Antibody

  • ceh-10 homeo domain containing homolog (C. elegans)
  • ceh-10 homeo domain containing homolog
  • ceh-10 homeodomain containing homolog (C. elegans)
  • Ceh-10 homeodomain-containing homolog
  • CHX10MCOPCB3
  • Homeobox protein CHX10
  • HOX10C elegans ceh-10 homeo domain-containing homolog
  • MCOP2
  • RET1
  • visual system homeobox 2

Background

The CHX10 gene encodes a homeobox protein originally described as a retina-specific transcription factor. Mutations in thisgene are associated with microphthalmia, cataracts and iris abnormalities. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CHX10 Antibody (NBP1-84476)(8)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 2 applications: ICC/IF, IHC-Fr.


Filter By Application
ICC/IF
(4)
IHC-Fr
(1)
All Applications
Filter By Species
Human
(3)
Rat
(1)
All Species
Showing Publications 1 - 8 of 8.
Publications using NBP1-84476 Applications Species
Joshua Hahn, Aboozar Monavarfeshani, Mu Qiao, Allison H. Kao, Yvonne Kölsch, Ayush Kumar, Vincent P. Kunze, Ashley M. Rasys, Rose Richardson, Joseph B. Wekselblatt, Herwig Baier, Robert J. Lucas, Wei Li, Markus Meister, Joshua T. Trachtenberg, Wenjun Yan, Yi-Rong Peng, Joshua R. Sanes, Karthik Shekhar Evolution of neuronal cell classes and types in the vertebrate retina Nature 2023-12-13 [PMID: 38092908]
Hahn J, Monavarfeshani A, Qiao M et al. Evolution of neuronal cell classes and types in the vertebrate retina bioRxiv : the preprint server for biology 2023-04-08 [PMID: 37066415] (IHC-Fr, Human)

Details:
Dilution: 1:50
IHC-Fr Human
Xu Z, Bai S, Wu H, Fang M Elevated retinal retinol-binding protein 4 levels in diabetic mice can induce retinal neurodegeneration through microglia Microscopy research and technique 2022-11-10 [PMID: 36354743]
Ke Y, Fan X, Hao R, et al. Human embryonic stem cell-derived extracellular vesicles alleviate retinal degeneration by upregulating Oct4 to promote retinal Müller cell retrodifferentiation via HSP90 Stem cell research & therapy 2021-01-07 [PMID: 33413616] (ICC/IF) ICC/IF
Ho R, Workman MJ, Mathkar P et al. Cross-Comparison of Human iPSC Motor Neuron Models of Familial and Sporadic ALS Reveals Early and Convergent Transcriptomic Disease Signatures Cell systems 2020-12-23 [PMID: 33382996]
Shrestha R, Wen YT, Ding DC, Tsai RK et al. Aberrant hiPSCs-Derived from Human Keratinocytes Differentiates into 3D Retinal Organoids that Acquire Mature Photoreceptors. Cells. 2019-03-09 [PMID: 30634512] (ICC/IF, Human) ICC/IF Human
Li Y, He X, Li J et al. Proliferation and differentiation of direct co-culture of bone marrow mesenchymal stem cells and pigmented cells from the ciliary margin Mol Med Rep 2017-01-01 [PMID: 28440470] (ICC/IF, Rat) ICC/IF Rat
Bejjani A, Choi MR, Cassidy L et al. RB116: An RB1+ retinoblastoma cell line expressing primitive markers. Mol Vis 2012-01-01 [PMID: 23233783] (ICC/IF, Human) ICC/IF Human

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional CHX10 Products

Array NBP1-84476

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Bioinformatics

Gene Symbol VSX2
Entrez
Uniprot