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PKR Antibody (HL71/10) [Unconjugated]

 (9 Publications)
 
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Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, K562 human chronic myelogenous leukemia cell line, and HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human PKR Monoclonal Antibody (Catalog # MAB1980) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for PKR at approximately 74 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, K562 human chronic myelogenous leukemia cell line, and HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1 µg/mL of ...read more
PKR was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line stimulated with rhIFN-alpha (Catalog # 11110-1) using Mouse Anti-Human PKR Monoclonal Antibody (Catalog # MAB1980) at 10 µg/mL for ...read more
Simple Western lane view shows lysates of K562 human chronic myelogenous leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for PKR at approximately 73 kDa (as indicated) using 10 µg/mL of ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IP, ICC/IF
Clone
HL71/10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price
Carrier Free
MAB1980-SP
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PKR Antibody (HL71/10) [Unconjugated] Summary

Immunogen
Protein kinase fraction from interferon-treated Daudi human Burkitt's lymphoma cell line. Laurent, A.G. et al. (1985) Proc. Natl. Acad. Sci. 82:4341.
Specificity
Detects human PKR in Western blots.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PRKRA
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
  • Immunoprecipitation
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
MAB1980 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PKR Antibody (HL71/10) [Unconjugated]

  • double stranded RNA activated protein kinase
  • EC 2.7.11.1
  • eIF-2A protein kinase 2
  • EIF2AK1
  • EIF2AK2
  • eukaryotic translation initiation factor 2-alpha kinase 2P1/eIF-2A protein kinase
  • interferon-induced, double-stranded RNA-activated protein kinase
  • interferon-inducible double stranded RNA dependent
  • interferon-inducible elF2alpha kinase
  • Interferon-inducible RNA-dependent protein kinase
  • P68 Kinase
  • PKR
  • PKRp68 kinase
  • PPP1R83
  • PRKR
  • PRKRMGC126524
  • Protein Kinase R
  • Protein kinase RNA-activated

Background

The interferon (IFN)-induced PKR mediates inhibition of protein synthesis through phosphorylation of the alpha subunit of eukaryotic inititation factor 2 (elF2alpha) and is also involved in the induction of the IFN gene through the activation of the transcription factor NF-kappa B.
  1. Bonnet, M.C. et al. (2000) Mol. Cell Biol. 20:4532.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PKR Antibody (MAB1980)(9)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: Immunoprecipitation, Western Blot.


Filter By Application
Immunoprecipitation
(4)
Western Blot
(3)
All Applications
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Human
(6)
All Species
Showing Publications 1 - 9 of 9.
Publications using MAB1980 Applications Species
Burnett, SB;Culver, AM;Simon, TA;Rowson, T;Frederick, K;Palmer, K;Murray, SA;Davis, SW;Patel, RC; A frameshift mutation in the murine Prkra gene causes dystonia and exhibits abnormal cerebellar development and reduced eIF2? phosphorylation bioRxiv : the preprint server for biology 2024-06-04 [PMID: 38895245] (Immunoprecipitation, Human) Immunoprecipitation Human
B Ukhueduan, E Chukwurah, RC Patel Regulation of PKR activation and apoptosis during oxidative stress by TRBP phosphorylation The international journal of biochemistry & cell biology, 2021-06-24;137(0):106030. 2021-06-24 [PMID: 34174402] (Immunoprecipitation, Western Blot, Human) Immunoprecipitation, Western Blot Human
Demy J. S. Kuipers, Wim Mandemakers, Chin‐Song Lu, Simone Olgiati, Guido J. Breedveld, Christina Fevga et al. EIF2AK2 Missense Variants Associated with Early Onset Generalized Dystonia Annals of Neurology 3/1/2021 [PMID: 33236446]
Sakiko Nishikawa, Yuka Itoh, Muneshige Tokugawa, Yasumichi Inoue, Ken-ichi Nakashima, Yuka Hori et al. Kurarinone from Sophora Flavescens Roots Triggers ATF4 Activation and Cytostatic Effects Through PERK Phosphorylation Molecules 2019-08-27 [PMID: 31461933]
E Golec, L Lind, M Qayyum, AM Blom, BC King The Noncoding RNA nc886 Regulates PKR Signaling and Cytokine Production in Human Cells J. Immunol., 2018-12-05;0(0):. 2018-12-05 [PMID: 30518569] (Western Blot, Human) Western Blot Human
Tatyana A. Shelkovnikova, Michail S. Kukharsky, Haiyan An, Pasquale Dimasi, Svetlana Alexeeva, Osman Shabir et al. Protective paraspeckle hyper-assembly downstream of TDP-43 loss of function in amyotrophic lateral sclerosis Molecular Neurodegeneration 12/1/2018 [PMID: 29859124]
E Chukwurah, RC Patel Stress-induced TRBP phosphorylation enhances its interaction with PKR to regulate cellular survival Sci Rep, 2018-01-18;8(1):1020. 2018-01-18 [PMID: 29348664] (Western Blot, Human) Western Blot Human
Vaughn L, Bragg D, Sharma N, Camargos S, Cardoso F, Patel R Altered activation of protein kinase PKR and enhanced apoptosis in dystonia cells carrying a mutation in PKR activator protein PACT. J Biol Chem, 2015-07-31;290(37):22543-57. 2015-07-31 [PMID: 26231208] (Immunoprecipitation, Human) Immunoprecipitation Human
Singh M, Fowlkes V, Handy I, Patel CV, Patel RC Essential role of PACT-mediated PKR activation in tunicamycin-induced apoptosis. J. Mol. Biol., 2008-11-05;385(2):457-68. 2008-11-05 [PMID: 19007793] (Immunoprecipitation, Human) Immunoprecipitation Human

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Bioinformatics

Gene Symbol PRKRA