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SIX6 Antibody

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Western Blot: SIX6 Antibody [NBP1-82478] - Analysis in human eye tissue.
Western Blot: SIX6 Antibody [NBP1-82478] - Analysis in human eye tissue.
Immunocytochemistry/ Immunofluorescence: SIX6 Antibody [NBP1-82478] - Staining of human cell line SH-SY5Y shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: SIX6 Antibody [NBP1-82478] - Staining of human pituitary gland shows moderate nuclear positivity.
Immunohistochemistry-Paraffin: SIX6 Antibody [NBP1-82478] -Staining of human cerebral cortex shows strong nuclear positivity in neurons.
Immunohistochemistry-Paraffin: SIX6 Antibody [NBP1-82478] - Staining of human skeletal muscle shows strong nuclear positivity in myocytes.
Immunohistochemistry-Paraffin: SIX6 Antibody [NBP1-82478] -Staining of human tonsil shows negative nuclear positivity in non-germinal center cells as expected.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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SIX6 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: YQEAEKLRGRPLGPVDKYRVRKKFPLPRTIWDGEQKTHCFKERTRNLLREWYLQDPYPNPSKKRELAQATGLTPTQVGNWFKNRRQRDRAAAAKNRLQQQVLSQGSGRALRAEGDGTPEVLGVATSPAASLSSKAATSAISITSSDSECDI
Predicted Species
Mouse (96%), Rat (96%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SIX6
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
SIX6 Recombinant Protein Antigen (NBP1-82478PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for SIX6 Antibody

  • homeobox protein SIX6
  • Homeodomain protein OPTX2
  • MCOPCT2
  • Optic homeobox 2
  • OPTX2
  • sine oculis homeobox (Drosophila) homolog 6
  • sine oculis homeobox homolog 6 (Drosophila)
  • Sine oculis homeobox homolog 6
  • SIX homeobox 6
  • Six9

Background

The protein encoded by the Homeobox protein SIX6 gene is a homeobox protein that is similar to the Drosophila 'sine oculis' gene product. This gene is found in a cluster of related genes on chromosome 14 and is thought to be involved in eye development. Defects in this gene are a cause of isolated microphthalmia with cataract type 2 (MCOPCT2). (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SIX6