Orthogonal Strategies: Immunohistochemistry-Paraffin: SIX1 Antibody [NBP1-84264] - Staining in human skeletal muscle and liver tissues . Corresponding SIX1 RNA-seq data are presented for the same tissues.
Genetic Strategies: Western Blot: SIX1 Antibody [NBP1-84264] - Analysis in Rh30 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading ...read more
Immunocytochemistry/ Immunofluorescence: SIX1 Antibody [NBP1-84264] - Staining of human cell line A-431 shows localization to nucleus & nucleoli. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: SIX1 Antibody [NBP1-84264] - Staining of human breast cancer shows weak to moderate nuclear positivity in tumor cells.
Immunohistochemistry-Paraffin: SIX1 Antibody [NBP1-84264] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: SIX1 Antibody [NBP1-84264] - Staining of human salivary gland shows moderate to strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: SIX1 Antibody [NBP1-84264] - Staining of human skeletal muscle shows strong nuclear positivity in striated muscle fibers.
This antibody was developed against Recombinant Protein corresponding to amino acids: CFKEKSRGVLREWYAHNPYPSPREKRELAEATGLTTTQVSNWFKNRRQRDRAAEAKERENTENNNSSSNKQNQLSPLEGGKPLMSSSEEEFSPPQSPDQNSVLLLQGNMGHARSSNYSLPGLTASQPSHGLQTHQHQLQDS
Predicted Species
Rat (99%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SIX1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Use in Mouse reported in scientific literature (PMID:24852826).
Packaging, Storage & Formulations
Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified
Alternate Names for SIX1 Antibody
BOS3
deafness, autosomal dominant 23
DFNA23
homeobox protein SIX1
sine oculis homeobox (Drosophila) homolog 1
sine oculis homeobox homolog 1 (Drosophila)
Sine oculis homeobox homolog 1
SIX homeobox 1
TIP39
Background
The protein encoded by the SIX1 gene is a homeobox protein that is similar to the Drosophila 'sine oculis' gene product. This gene is found in a cluster of related genes on chromosome 14 and is thought to be involved in limb development. Defects in this gene are a cause of autosomal dominant deafness type 23 (DFNA23) and branchiootic syndrome type 3 (BOS3). (provided by RefSeq)
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
=
÷
Review this Product
Be the first to review our SIX1 Antibody and receive a gift card or discount.
![Immunohistochemistry-Paraffin: SIX1 Antibody [NBP1-84264] - Staining in human skeletal muscle and liver tissues . Corresponding SIX1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/SIX1-Antibody-Immunohistochemistry-Paraffin-NBP1-84264-img0025.jpg "Immunohistochemistry-Paraffin: SIX1 Antibody [NBP1-84264] - Staining in human skeletal muscle and liver tissues . Corresponding SIX1 RNA-seq data are presented for the same tissues.")
![Immunohistochemistry PTH Antibody (918462) [Unconjugated]](https://images.novusbio.com/images2/PTH_MAB7665_Immunohistochemistry_16650.jpg)
![Western Blot PTH Antibody (918462) [Unconjugated]](https://images.novusbio.com/images2/PTH_MAB7665_Western_Blot_16656.jpg)
![Western Blot: SIX1 Antibody [NBP1-84264] - Analysis in Rh30 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/SIX1-Antibody-Western-Blot-NBP1-84264-img0020.jpg "Western Blot: SIX1 Antibody [NBP1-84264] - Analysis in Rh30 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-PPIB.")
![Immunocytochemistry/Immunofluorescence: SIX1 Antibody [NBP1-84264] - Staining of human cell line A-431 shows localization to nucleus & nucleoli. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/SIX1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84264-img0012.jpg "Immunocytochemistry/Immunofluorescence: SIX1 Antibody [NBP1-84264] - Staining of human cell line A-431 shows localization to nucleus & nucleoli. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: SIX1 Antibody [NBP1-84264] - Staining of human breast cancer shows weak to moderate nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/SIX1-Antibody-Immunohistochemistry-Paraffin-NBP1-84264-img0021.jpg "Immunohistochemistry-Paraffin: SIX1 Antibody [NBP1-84264] - Staining of human breast cancer shows weak to moderate nuclear positivity in tumor cells.")
![Immunohistochemistry-Paraffin: SIX1 Antibody [NBP1-84264] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/SIX1-Antibody-Immunohistochemistry-Paraffin-NBP1-84264-img0022.jpg "Immunohistochemistry-Paraffin: SIX1 Antibody [NBP1-84264] - Staining of human liver shows no positivity in hepatocytes as expected.")
![Immunohistochemistry-Paraffin: SIX1 Antibody [NBP1-84264] - Staining of human salivary gland shows moderate to strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/SIX1-Antibody-Immunohistochemistry-Paraffin-NBP1-84264-img0023.jpg "Immunohistochemistry-Paraffin: SIX1 Antibody [NBP1-84264] - Staining of human salivary gland shows moderate to strong nuclear positivity in glandular cells.")
![Immunohistochemistry-Paraffin: SIX1 Antibody [NBP1-84264] - Staining of human skeletal muscle shows strong nuclear positivity in striated muscle fibers.](http://images.novusbio.com/fullsize/SIX1-Antibody-Immunohistochemistry-Paraffin-NBP1-84264-img0024.jpg "Immunohistochemistry-Paraffin: SIX1 Antibody [NBP1-84264] - Staining of human skeletal muscle shows strong nuclear positivity in striated muscle fibers.")
![Immunohistochemistry PTHLH/PTHrP Antibody (677939) [Unconjugated]](https://images.novusbio.com/images2/PTHLH_MAB6734_Immunohistochemistry_10816.jpg)
![Immunocytochemistry Pax3/Pax7 Antibody (274212) [Unconjugated]](https://images.novusbio.com/images2/Pax3_MAB2457_Immunocytochemistry__Immunofluorescence_20313.jpg)
![Immunocytochemistry/ Immunofluorescence Pax3/Pax7 Antibody (274212) [Unconjugated]](https://images.novusbio.com/images/mab2457_human-mouse-pax3-pax7-mab-clone-274212-41202412512639.jpg)
![Immunocytochemistry Pax2 Antibody [Unconjugated]](https://images.novusbio.com/images2/Pax2_AF3364_Immunocytochemistry_14405.jpg)
![Western Blot Pax2 Antibody [Unconjugated]](https://images.novusbio.com/images2/af3364_human-pax2-affinity-purified-polyclonal-ab-western-blot-145202183852.jpg)
![Immunohistochemistry Pax2 Antibody [Unconjugated]](https://images.novusbio.com/images2/af3364_human-pax2-affinity-purified-polyclonal-ab-immunohistochemistry-192021142455.jpg)
![Simple Western Pax6 Antibody [Unconjugated]](https://images.novusbio.com/images2/Pax6_AF8150_Simple_Western_17126.jpg)
![Immunocytochemistry Pax6 Antibody [Unconjugated]](https://images.novusbio.com/images2/Pax6_AF8150_Immunocytochemistry_14427.jpg)
![Western Blot Pax6 Antibody [Unconjugated]](https://images.novusbio.com/images2/Pax6_AF8150_Western_Blot_14743.jpg)
![SDS-PAGE BMP-4 [Unconjugated]](https://images.novusbio.com/images2/BMP-4_314-BP_100.jpg)
![Bioactivity BMP-4 [Unconjugated]](https://images.novusbio.com/images2/314-bp_recombinant-human-bmp-4-protein-1882021123744.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")