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Smad1 Antibody [Unconjugated]

 (8 Publications)
 
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Smad1 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Goat Anti-Human Smad1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2039) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the nuclei of glandular epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Smad1 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Goat Anti-Human Smad1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2039) at 15 µg/mL overnight at ...read more
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, MBA-MB-468 human breast cancer cell line, and HT-29 human colon adenocarcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat ...read more
Smad1 was detected in immersion fixed paraffin-embedded sections of human breast array using Goat Anti-Human Smad1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2039) at 15 µg/mL overnight at 4 °C. Tissue ...read more
Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line and MDA‑MB‑468 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for Smad1 at approximately 66 ...read more
Up-regulation of BMP signaling in the Fstl1-/- ureter and kidney.(A) Western blots of pSmad1/5/8, Smad1, pAKT (Ser473), AKT, and beta -actin from E15.5 (left panel) and E16.5 (second panel) ureter protein, E18.5 kidney ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF2039-SP
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Smad1 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Smad1
Asn2-Met454
Accession # Q15797
Specificity
Detects human Smad1 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human (rh) Smad5 is observed, and less than 5% cross-reactivity rhSmad4 and rhSmad9 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SMAD1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 5 ug/mL
  • Western Blot 0.5 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Publications
Read Publications using
AF2039 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Smad1 Antibody [Unconjugated]

  • BSP1
  • BSP-1
  • hSMAD1
  • JV4-1SMAD, mothers against DPP homolog 1 (Drosophila)
  • MAD homolog 1
  • MADH1JV41
  • MADR1MAD, mothers against decapentaplegic homolog 1 (Drosophila)
  • Mad-related protein 1
  • mothers against decapentaplegic homolog 1
  • Mothers against DPP homolog 1
  • SMAD family member 1SMAD 1
  • SMAD, mothers against DPP homolog 1
  • Smad1
  • TGF-beta signaling protein 1
  • transforming growth factor-beta signaling protein 1

Background

Smads are a family of intracellular proteins that transmit transforming growth factor beta (TGF-beta ) superfamily signals from the cell surface to the nucleus. Upon signaling by some BMP family members, Smad1 is phosphorylated resulting in its association with the common-mediator subunit, Smad4. This heteromeric complex then translocates into the nucleus to exert transcriptional comodulator activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Smad1 Antibody (AF2039)(8)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: IHC, Western Blot.


Filter By Application
IHC
(1)
Western Blot
(4)
All Applications
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Human
(4)
Mouse
(1)
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Showing Publications 1 - 8 of 8.
Publications using AF2039 Applications Species
E Dalmo, P Johansson, M Niklasson, I Gustavsson, S Nelander, B Westermark Growth inhibitory activity of bone morphogenetic protein 4 in human glioblastoma cell lines is heterogeneous and dependent on reduced SOX2 expression Mol. Cancer Res., 2020-03-31;0(0):. 2020-03-31 [PMID: 32234828] (Western Blot, Human) Western Blot Human
Toshio Doi, Tatsumi Moriya, Yui Fujita, Naoto Minagawa, Masaru Usami, Tomoko Sasaki et al. Urinary IgG4 and Smad1 Are Specific Biomarkers for Renal Structural and Functional Changes in Early Stages of Diabetic Nephropathy Diabetes 2018-05-01 [PMID: 29490904]
Karystinou A, Roelofs AJ, Neve A et al. Yes-associated protein (YAP) is a negative regulator of chondrogenesis in mesenchymal stem cells. Arthritis Res Ther. 5/30/2015 [PMID: 26025096]
Jiang D, Aguiar R MicroRNA-155 controls RB phosphorylation in normal and malignant B lymphocytes via the noncanonical TGF-beta1/SMAD5 signaling module. Blood, 2013-10-17;123(1):86-93. 2013-10-17 [PMID: 24136167] (Western Blot, Human) Western Blot Human
Shimamoto , Seiko, Kubota , Yasuo, Yamaguchi , Fuminori, Tokumitsu , Hiroshi, Kobayashi , Ryoji Ca2+/S100 proteins act as upstream regulators of the chaperone-associated ubiquitin ligase CHIP (C terminus of Hsc70-interacting protein). J Biol Chem, 2013-01-23;288(10):7158-68. 2013-01-23 [PMID: 23344957] (Western Blot, Human) Western Blot Human
Olivier Nolan-Stevaux, Wendy Zhong, Stacey Culp, Kathy Shaffer, Joseph Hoover, Dineli Wickramasinghe et al. Endoglin Requirement for BMP9 Signaling in Endothelial Cells Reveals New Mechanism of Action for Selective Anti-Endoglin Antibodies PLoS ONE 2012-12-27 [PMID: 23300529]
Alborzinia H, Schmidt-Glenewinkel H, Ilkavets I, Breitkopf-Heinlein K, Cheng X, Hortschansky P, Dooley S, Wolfl S Quantitative kinetics analysis of BMP2 uptake into cells and its modulation by BMP antagonists. J Cell Sci, 2012-10-17;126(0):117-27. 2012-10-17 [PMID: 23077176] (Western Blot, Human) Western Blot Human
Xu J, Qi X, Gong J Fstl1 antagonizes BMP signaling and regulates ureter development. PLoS ONE, 2012-04-02;7(4):e32554. 2012-04-02 [PMID: 22485132] (IHC, Mouse) IHC Mouse

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Bioinformatics

Gene Symbol SMAD1
Uniprot