| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Simple Western, IHC |
| Clone | 293039 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | LYOPH |
| Immunogen | E. coli-derived recombinant human Smad7 Gly320-Ser398 Accession # O15105 |
| Specificity | Detects human, mouse, and rat Smad7. |
| Source | N/A |
| Isotype | IgG2b |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | SMAD7 |
| Purity Statement | Protein A or G purified from hybridoma culture supernatant |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
|
| Reviewed Applications |
|
|
| Publications |
|
| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
| Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Reconstitution Instructions | Reconstitute at 0.5 mg/mL in sterile PBS. |
Smads are a family of intracellular proteins that transmit transforming growth factor beta (TGF-beta ) superfamily signals from the cell surface to the nucleus. The Smad family is divided into three subclasses: receptor regulated Smads, (Smads 1, 2, 3, 5 and 8); the common partner, (Smad4) that functions via its interaction to the various Smads; and the inhibitory Smads, (Smads 6 and 7). Smad7, also known as Mothers Against Decapentaplegic homolog 7 (MADH7), inhibits selected pathways by binding directly to cell-surface receptors and preventing the activation-induced phosphorylation of other Smad subunits.
| Images | Ratings | Applications | Species | Date | Details | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
|
reviewed by:
Verified Customer |
WB | Mouse | 02/16/2015 |
Summary
|
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![TGF‐ beta 1 signaling in MSCs is regulated by environmental TGF‐ beta 1 concentrationAAT‐MSCs were cultured in the labeling media containing [35S]‐Met/Cys and increasing concentrations of r.h. TGF‐ beta 1 at 0, 0.1, 0.3, 1, 3, and 10 ng/ml for overnight. Supernatants were harvested and incubated with TGF‐ beta 1 capture antibody. After washing, the captured TGF‐ beta 1 was transferred to glass filter membrane, and the radioactivity was counted with a scintillation counter (black bars). The supernatants were incubated with new TGF‐ beta 1 capture antibody, and the radioactivity of captured TGF‐ beta 1 was counted again (white bars). The data are given as mean ± SD of five repeated measurements. This experiment was performed four times with comparable results. CPM, count per minute.B, CAT‐MSCs (B) or HDFs (C) were cultured in media containing increasing concentrations of r.h. TGF‐ beta 1 at 0, 0.1, 0.3, 1, 3, and 10 ng/ml for 24 h, before protein lysates or total RNAs were harvested. Western blot analysis of Smad7 was performed with protein lysates and semi‐quantitatively analyzed with densitometry. beta ‐actin served as loading control. Data are given as mean ± SEM of three independent blots. **P < 0.01, by one‐way ANOVA with Tukey's test. AU, arbitrary unit.DFrom total RNA, the mature miR‐21 and endogenous control RNU6B were converted to cDNA and quantified by qPCR‐based TaqMan microRNA assays. The expression of miR‐21 was normalized on RNU6B. Data are given as mean ± SEM of three independent experiments.Source data are available online for this figure. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32080965), licensed under a CC-BY license. Not internally tested by R&D Systems.](http://images.novusbio.com/fullsize/mab2029_human-mouse-rat-smad7-mab-clone-293039-41202410484331.jpg "TGF‐ beta 1 signaling in MSCs is regulated by environmental TGF‐ beta 1 concentrationAAT‐MSCs were cultured in the labeling media containing [35S]‐Met/Cys and increasing concentrations of r.h. TGF‐ beta 1 at 0, 0.1, 0.3, 1, 3, and 10 ng/ml for overnight. Supernatants were harvested and incubated with TGF‐ beta 1 capture antibody. After washing, the captured TGF‐ beta 1 was transferred to glass filter membrane, and the radioactivity was counted with a scintillation counter (black bars). The supernatants were incubated with new TGF‐ beta 1 capture antibody, and the radioactivity of captured TGF‐ beta 1 was counted again (white bars). The data are given as mean ± SD of five repeated measurements. This experiment was performed four times with comparable results. CPM, count per minute.B, CAT‐MSCs (B) or HDFs (C) were cultured in media containing increasing concentrations of r.h. TGF‐ beta 1 at 0, 0.1, 0.3, 1, 3, and 10 ng/ml for 24 h, before protein lysates or total RNAs were harvested. Western blot analysis of Smad7 was performed with protein lysates and semi‐quantitatively analyzed with densitometry. beta ‐actin served as loading control. Data are given as mean ± SEM of three independent blots. **P < 0.01, by one‐way ANOVA with Tukey's test. AU, arbitrary unit.DFrom total RNA, the mature miR‐21 and endogenous control RNU6B were converted to cDNA and quantified by qPCR‐based TaqMan microRNA assays. The expression of miR‐21 was normalized on RNU6B. Data are given as mean ± SEM of three independent experiments.Source data are available online for this figure. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32080965), licensed under a CC-BY license. Not internally tested by R&D Systems.")
![TGF‐ beta 1 signaling in MSCs is regulated by environmental TGF‐ beta 1 concentrationAAT‐MSCs were cultured in the labeling media containing [35S]‐Met/Cys and increasing concentrations of r.h. TGF‐ beta 1 at 0, 0.1, 0.3, 1, 3, and 10 ng/ml for overnight. Supernatants were harvested and incubated with TGF‐ beta 1 capture antibody. After washing, the captured TGF‐ beta 1 was transferred to glass filter membrane, and the radioactivity was counted with a scintillation counter (black bars). The supernatants were incubated with new TGF‐ beta 1 capture antibody, and the radioactivity of captured TGF‐ beta 1 was counted again (white bars). The data are given as mean ± SD of five repeated measurements. This experiment was performed four times with comparable results. CPM, count per minute.B, CAT‐MSCs (B) or HDFs (C) were cultured in media containing increasing concentrations of r.h. TGF‐ beta 1 at 0, 0.1, 0.3, 1, 3, and 10 ng/ml for 24 h, before protein lysates or total RNAs were harvested. Western blot analysis of Smad7 was performed with protein lysates and semi‐quantitatively analyzed with densitometry. beta ‐actin served as loading control. Data are given as mean ± SEM of three independent blots. **P < 0.01, by one‐way ANOVA with Tukey's test. AU, arbitrary unit.DFrom total RNA, the mature miR‐21 and endogenous control RNU6B were converted to cDNA and quantified by qPCR‐based TaqMan microRNA assays. The expression of miR‐21 was normalized on RNU6B. Data are given as mean ± SEM of three independent experiments.Source data are available online for this figure. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32080965), licensed under a CC-BY license. Not internally tested by R&D Systems.](http://images.novusbio.com/fullsize/mab2029_human-mouse-rat-smad7-mab-clone-293039-4120241049448.jpg "TGF‐ beta 1 signaling in MSCs is regulated by environmental TGF‐ beta 1 concentrationAAT‐MSCs were cultured in the labeling media containing [35S]‐Met/Cys and increasing concentrations of r.h. TGF‐ beta 1 at 0, 0.1, 0.3, 1, 3, and 10 ng/ml for overnight. Supernatants were harvested and incubated with TGF‐ beta 1 capture antibody. After washing, the captured TGF‐ beta 1 was transferred to glass filter membrane, and the radioactivity was counted with a scintillation counter (black bars). The supernatants were incubated with new TGF‐ beta 1 capture antibody, and the radioactivity of captured TGF‐ beta 1 was counted again (white bars). The data are given as mean ± SD of five repeated measurements. This experiment was performed four times with comparable results. CPM, count per minute.B, CAT‐MSCs (B) or HDFs (C) were cultured in media containing increasing concentrations of r.h. TGF‐ beta 1 at 0, 0.1, 0.3, 1, 3, and 10 ng/ml for 24 h, before protein lysates or total RNAs were harvested. Western blot analysis of Smad7 was performed with protein lysates and semi‐quantitatively analyzed with densitometry. beta ‐actin served as loading control. Data are given as mean ± SEM of three independent blots. **P < 0.01, by one‐way ANOVA with Tukey's test. AU, arbitrary unit.DFrom total RNA, the mature miR‐21 and endogenous control RNU6B were converted to cDNA and quantified by qPCR‐based TaqMan microRNA assays. The expression of miR‐21 was normalized on RNU6B. Data are given as mean ± SEM of three independent experiments.Source data are available online for this figure. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32080965), licensed under a CC-BY license. Not internally tested by R&D Systems.")
![Western Blot Smad2/3 Antibody [Unconjugated]](https://images.novusbio.com/images2/Smad3_AF3797_Western_Blot_5347.jpg)
![Chromatin Immunoprecipitation (ChIP) Smad2/3 Antibody [Unconjugated]](https://images.novusbio.com/images2/Smad3_AF3797_Chromatin_Immunoprecipitation_11240.jpg)
![Immunocytochemistry Smad2/3 Antibody [Unconjugated]](https://images.novusbio.com/images2/Smad3_AF3797_Immunocytochemistry_11829.jpg)
![Chromatin Immunoprecipitation (ChIP) Smad4 Antibody [Unconjugated]](https://images.novusbio.com/images2/Smad4_AF2097_Chromatin_Immunoprecipitation_11225.jpg)
![Western Blot Smad4 Antibody [Unconjugated]](https://images.novusbio.com/images2/Smad4_AF2097_Western_Blot_17168.jpg)
![Immunocytochemistry Smad4 Antibody [Unconjugated]](https://images.novusbio.com/images2/Smad4_AF2097_Immunocytochemistry__Immunofluorescence_17807.jpg)
![Bioactivity TGF-beta 1 [Unconjugated]](https://images.novusbio.com/images/protein/7754-bhcf_recombinant-human-tgf-beta-1-human-cell-expressed-cf-bioactivity-1811202013946.jpg)
![Western Blot TGF-beta RI/ALK-5 Antibody [Unconjugated]](https://images.novusbio.com/images2/TGFbeta_RI_AF3025_Western_Blot_17081.jpg)
![Immunocytochemistry TGF-beta RI/ALK-5 Antibody [Unconjugated]](https://images.novusbio.com/images2/TGF-beta_RI_AF3025_Immunocytochemistry_9948.jpg)
![Western Blot TGF-beta RI/ALK-5 Antibody [Unconjugated]](https://images.novusbio.com/images/af3025_human-tgf-beta-ri-alk-5-affinity-purified-polyclonal-ab-41202412402510.jpg)
![Immunohistochemistry CTGF/CCN2 Antibody [Unconjugated] - C-terminus](https://images.novusbio.com/images2/CTGF_AF660_Immunohistochemistry_9968.jpg)
![Immunohistochemistry CTGF/CCN2 Antibody [Unconjugated] - C-terminus](https://images.novusbio.com/images2/CTGF_AF660_Immunohistochemistry_9969.jpg)
![Western Blot CTGF/CCN2 Antibody [Unconjugated] - C-terminus](https://images.novusbio.com/images/af660_human-ctgf-ccn2-c-terminus-affinity-purified-polyclonal-ab-822024951141.jpg)
![Immunohistochemistry Smad1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Smad1_AF2039_Immunohistochemistry_10106.jpg)
![Western Blot Smad1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Smad1_AF2039_Western_Blot_5162.jpg)
![Simple Western Smad1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Smad1_AF2039_Simple_Western_16677.jpg)
![Western Blot ERK2 Antibody [Unconjugated]](https://images.novusbio.com/images2/ERK2_AF1230_Western_Blot_5097.jpg)
![Immunohistochemistry ERK2 Antibody [Unconjugated]](https://images.novusbio.com/images2/ERK2_AF1230_Immunohistochemistry_20696.jpg)
![Knockout Validated ERK2 Antibody [Unconjugated]](https://images.novusbio.com/images2/ERK2_AF1230_Knockout_Validated_22864.jpg)
![SDS-PAGE TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg)
![Bioactivity TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg)
![SEC-MALS TNF-alpha [Unconjugated]](https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg)
![Bioactivity BMP-2 [Unconjugated]](https://images.novusbio.com/images/protein/355-bm_recombinant-human-mouse-rat-bmp-2-protein-bioactivity-181120209742.jpg)
![Western Blot c-jun [p Ser63] Antibody (1018Y) [Unconjugated]](https://images.novusbio.com/images2/cJun_MAB8930_Western_Blot_18923.jpg)
![Immunocytochemistry c-jun [p Ser63] Antibody (1018Y) [Unconjugated]](https://images.novusbio.com/images2/cJun_MAB8930_Immunocytochemistry__Immunofluorescence_18945.jpg)
![Simple Western c-jun [p Ser63] Antibody (1018Y) [Unconjugated]](https://images.novusbio.com/images2/cJun_MAB8930_Simple_Western_19175.jpg)
