Name: Smad2/3 Antibody [Unconjugated]
Brand: Novus Biologicals
SKU: AF3797
Rating: 4.5 (2 reviews)

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	Hu, MuSpecies Glossary
Applications	WB, Simple Western, IHC, ChIP, ICC/IF
Clonality	Polyclonal
Host	Goat
Conjugate	Unconjugated
Concentration	LYOPH

Catalog# & Formulation	Size	Price
Carrier Free AF3797-SP

Immunogen	E. coli-derived recombinant human Smad3 Ser2-Ala230 Accession # P84022
Specificity	Detects human, mouse, and rat Smad2/3 in Western blots. Predicted to detect rat based on sequence homology.
Source	N/A
Isotype	IgG
Clonality	Polyclonal
Host	Goat
Gene	SMAD2
Purity Statement	Antigen Affinity-purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative	No Preservative
Concentration	LYOPH
Reconstitution Instructions	Reconstitute at 0.2 mg/mL in sterile PBS.

Smad2/3 Antibody [Unconjugated]

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Publications for Smad2/3 Antibody (AF3797)(24)

Reviews for Smad2/3 Antibody (AF3797) (2) 4.52

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Publications using AF3797	Applications	Species
Militi, S;Nibhani, R;Jalali, M;Pauklin, S; RBL2-E2F-GCN5 guide cell fate decisions during tissue specification by regulating cell-cycle-dependent fluctuations of non-cell-autonomous signaling Cell reports 2023-09-18 [PMID: 37725511] (ICC, Human)	ICC	Human
Mouti MA, Deng S, Pook M et al. KMT2A associates with PHF5A-PHF14-HMG20A-RAI1 subcomplex in pancreatic cancer stem cells and epigenetically regulates their characteristics Nature communications 9/14/2023 [PMID: 37709746]
Yoshida M, Kokubun T, Sato K et al. DPP-4 Inhibitors Attenuate Fibrosis After Glaucoma Filtering Surgery by Suppressing the TGF-?/Smad Signaling Pathway Investigative ophthalmology & visual science 7/3/2023 [PMID: 37405760]
RA Razali, MD Yazid, A Saim, RBH Idrus, Y Lokanathan Approaches in Hydroxytyrosol Supplementation on Epithelial-Mesenchymal Transition in TGFbeta1-Induced Human Respiratory Epithelial Cells International Journal of Molecular Sciences, 2023-02-16;24(4):. 2023-02-16 [PMID: 36835384] (Simple Western, Human)	Simple Western	Human
M Sisto, D Ribatti, G Ingravallo, S Lisi The Expression of Follistatin-like 1 Protein Is Associated with the Activation of the EMT Program in Sjï¿½gren's Syndrome Journal of Clinical Medicine, 2022-09-13;11(18):. 2022-09-13 [PMID: 36143013] (Western Blot, Human)	Western Blot	Human
Rebecca L. Mather, Abhijit Parolia, Sandra E. Carson, Erik Venalainen, David Roig‐Carles, Mustapha Jaber et al. The evolutionarily conserved long non‐coding RNA LINC00261 drives neuroendocrine prostate cancer proliferation and metastasis via distinct nuclear and cytoplasmic mechanisms Molecular Oncology 7/1/2021 [PMID: 33793068]
LD Halder, EAH Jo, MZ Hasan, M Ferreira-G, T Krüger, M Westermann, DI Palme, G Rambach, N Beyersdorf, C Speth, ID Jacobsen, O Kniemeyer, B Jungnickel, PF Zipfel, C Skerka Immune modulation by complement receptor 3-dependent human monocyte TGF-beta1-transporting vesicles Nat Commun, 2020-05-11;11(1):2331. 2020-05-11 [PMID: 32393780] (Western Blot, Human)	Western Blot	Human
Chen PY, Qin L, Li G et al. Smooth Muscle Cell Reprogramming in Aortic Aneurysms Cell Stem Cell 4/2/2020 [PMID: 32243809]
L Wang, Y Li, X Wang, P Wang, K Essandoh, S Cui, W Huang, X Mu, Z Liu, Y Wang, T Peng, GC Fan GDF3 Protects Mice against Sepsis-Induced Cardiac Dysfunction and Mortality by Suppression of Macrophage Pro-Inflammatory Phenotype Cells, 2020-01-03;9(1):. 2020-01-03 [PMID: 31947892] (Mouse)		Mouse
M Sisto, L Lorusso, G Ingravallo, D Ribatti, S Lisi TGFbeta1-Smad canonical and -Erk noncanonical pathways participate in interleukin-17-induced epithelial-mesenchymal transition in Sjï¿½gren's syndrome Lab. Invest., 2020-01-10;100(6):824-836. 2020-01-10 [PMID: 31925325] (Flow Cytometry, Western Blot, Human)	Flow Cytometry, Western Blot	Human
J Jang, D Han, M Golkaram, M Audouard, G Liu, D Bridges, S Hellander, A Chialastri, SS Dey, LR Petzold, KS Kosik Control over single-cell distribution of G1 lengths by WNT governs pluripotency PLoS Biol., 2019-09-26;17(9):e3000453. 2019-09-26 [PMID: 31557150] (Western Blot, Human)	Western Blot	Human
GE Gentsch, T Spruce, NDL Owens, JC Smith Maternal pluripotency factors initiate extensive chromatin remodelling to predefine first response to inductive signals Nat Commun, 2019-09-19;10(1):4269. 2019-09-19 [PMID: 31537794] (ChIP, IHC, Xenopus)	ChIP, IHC	Xenopus
He R, Wang M, Zhao C et al. TFEB-driven autophagy potentiates TGF-beta induced migration in pancreatic cancer cells J. Exp. Clin. Cancer Res. 8/6/2019 [PMID: 31387632]
K Nakai, S Karita, J Igarashi, I Tsukamoto, K Hirano, Y Kubota COA-Cl prevented TGF-?1-induced CTGF expression by Akt dephosphorylation in normal human dermal fibroblasts, and it attenuated skin fibrosis in mice models of systemic sclerosis J. Dermatol. Sci., 2019-03-12;0(0):. 2019-03-12 [PMID: 30954335] (Western Blot, Mouse)	Western Blot	Mouse
A Bertero, S Brown, P Madrigal, A Osnato, D Ortmann, L Yiangou, J Kadiwala, NC Hubner, IR de Los Moz, C Sadée, AS Lenaerts, S Nakanoh, R Grandy, E Farnell, J Ule, HG Stunnenber, S Mendjan, L Vallier The SMAD2/3 interactome reveals that TGF? controls m6A mRNA methylation in pluripotency Nature, 2018-02-28;555(7695):256-259. 2018-02-28 [PMID: 29489750] (Immunoprecipitation, Human)	Immunoprecipitation	Human
MS Ventura Fe, M Bienert, K Müller, B Rath, T Goecke, C Opländer, T Braunschwe, P Mela, TH Brümmendor, F Beier, S Neuss Comprehensive characterization of chorionic villi-derived mesenchymal stromal cells from human placenta Stem Cell Res Ther, 2018-02-05;9(1):28. 2/5/2018 [PMID: 29402304]
Tao Cheng Neuronal Protein 3.1 Deficiency Leads to Reduced Cutaneous Scar Collagen Deposition and Tensile Strength due to Impaired Transforming Growth Factor-?1 to -?3 Translation Am. J. Pathol, 2016-12-08;0(0):. 2016-12-08 [PMID: 27939132] (Western Blot, Mouse)	Western Blot	Mouse
Potential mechanisms underlying ectodermal differentiation of Wharton's jelly mesenchymal stem cells Biochem Biophys Res Commun, 2016-08-05;0(0):. 2016-08-05 [PMID: 27501759] (Western Blot, Human)	Western Blot	Human
Yoon , Se-Jin, Foley , Joseph W, Baker , Julie C HEB associates with PRC2 and SMAD2/3 to regulate developmental fates. Nat Commun, 2015-03-16;6(0):6546. 2015-03-16 [PMID: 25775035] (ChIP, Mouse)	ChIP	Mouse
Rosa A, Papaioannou M, Krzyspiak J, Brivanlou A miR-373 is regulated by TGFbeta signaling and promotes mesendoderm differentiation in human Embryonic Stem Cells. Dev Biol, 2014-04-04;391(1):81-8. 2014-04-04 [PMID: 24709321] (ChIP, Human)	ChIP	Human
Pereira P, Dobreva M, Maas E, Cornelis F, Moya I, Umans L, Verfaillie C, Camus A, de Sousa Lopes S, Huylebroeck D, Zwijsen A Antagonism of Nodal signaling by BMP/Smad5 prevents ectopic primitive streak formation in the mouse amnion. Development, 2012-09-01;139(18):3343-54. 2012-09-01 [PMID: 22912414] (Immunoprecipitation, Human)	Immunoprecipitation	Human
Kudryashova E, Kramerova I, Spencer MJ Satellite cell senescence underlies myopathy in a mouse model of limb-girdle muscular dystrophy 2H. J. Clin. Invest., 2012-04-16;122(5):1764-76. 2012-04-16 [PMID: 22505452] (Western Blot, Mouse)	Western Blot	Mouse
Kim SW, Yoon SJ, Chuong E, Oyolu C, Wills AE, Gupta R, Baker J Chromatin and transcriptional signatures for Nodal signaling during endoderm formation in hESCs. Dev. Biol., 2011-06-29;357(2):492-504. 2011-06-29 [PMID: 21741376] (ChIP, Human)	ChIP	Human
Zakrzewicz A, Kouri FM, Nejman B, Kwapiszewska G, Hecker M, Sandu R, Dony E, Seeger W, Schermuly RT, Eickelberg O, Morty RE The transforming growth factor-beta/Smad2,3 signalling axis is impaired in experimental pulmonary hypertension. Eur. Respir. J., 2007-03-28;29(6):1094-104. 2007-03-28 [PMID: 17392319] (IHC, Rat)	IHC	Rat
Show All 24 Publications. Collapse Publications.

	KARTHIKEYAN THIRUGNANAM 12/20/2024
Application:	WB
Species:	Mouse

	Verified Customer 01/08/2015
Application:	WB
Species:	Mouse

Gene Symbol	SMAD2
Uniprot	P84022()