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Smad4 Antibody (253343) [Unconjugated]

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Smad4 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human Smad4 Monoclonal Antibody (Catalog # MAB2097) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Smad4 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human Smad4 Monoclonal Antibody (Catalog # MAB2097) at 3 µg/mL for 3 hours at room temperature. Cells ...read more
HeLa human cell line was stained with Mouse Anti-Human Smad4 Monoclonal Antibody (Catalog # MAB2097, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications CyTOF-ready, Flow, ICC/IF
Clone
253343
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
IC2097U-100UG
Alexa Fluor 405
IC2097V-100UG
Alexa Fluor 488
IC2097G-100UG
Alexa Fluor 532
FAB2097X
Alexa Fluor 594
IC2097T-100UG
Alexa Fluor 647
IC2097R-100UG
Alexa Fluor 700
IC2097N-100UG
Alexa Fluor 750
IC2097S-100UG
Allophycocyanin
FAB2097A
Biotin
FAB2097B
CoraFluor 1
FAB2097CL1
DyLight 350
FAB2097D
DyLight 405
FAB2097E
DyLight 488
FAB2097K
DyLight 550
FAB2097L
DyLight 594
FAB2097M
DyLight 650
FAB2097W
DyLight 680
FAB2097Y
DyLight 755
FAB2097Z
FITC
FAB2097F
HRP
FAB2097H
Janelia Fluor 525
FAB2097JF525
Janelia Fluor 549
FAB2097I
Janelia Fluor 646
FAB2097J
Janelia Fluor 669
FAB2097JF669
PerCP
FAB2097C
Phycoerythrin
IC2097P
mFluor Violet 450 SE
FAB2097MFV450
mFluor Violet 500 SE
FAB2097MFV500
mFluor Violet 610 SE
FAB2097MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
IC2097P
Carrier Free
MAB2097-SP
Carrier Free
IC2097T-100UG
Carrier Free
IC2097S-100UG
Carrier Free
IC2097R-100UG
Carrier Free
IC2097N-100UG
Carrier Free
IC2097G-100UG
Carrier Free
IC2097V-100UG
Carrier Free
IC2097U-100UG
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Smad4 Antibody (253343) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Smad4
Pro139-Asp332
Accession # Q13485
Specificity
Detects human Smad4 in direct ELISAs.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
SMAD4
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Immunocytochemistry 3-25 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
Publications
Read Publication using
MAB2097 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is covered by one or more of the following US patents: 5,712,097; 5,814,457; 5,955,292 and other pending US patent applications and foreign equivalents.



This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Smad4 Antibody (253343) [Unconjugated]

  • DPC4
  • DPC4SMAD, mothers against DPP homolog 4 (Drosophila)
  • hSMAD4
  • JIP
  • MAD homolog 4
  • MADH4
  • MADH4mothers against decapentaplegic homolog 4
  • mothers against decapentaplegic homolog 4
  • mothers against decapentaplegic, Drosophila, homolog of, 4
  • SMAD 4
  • SMAD family member 4MAD, mothers against decapentaplegic homolog 4 (Drosophila)
  • SMAD, mothers against DPP homolog 4
  • Smad4

Background

Mothers Against Decapentaplegic homolog 4 (Smad4) belongs to a family of intracellular proteins that transmit transforming growth factor beta (TGF-beta ) superfamily signals from the cell surface to the nucleus. Upon signal-induced phosphorylation, Smad subunits associate with the common-mediator subunit, Smad4. This heteromeric complex then translocates into the nucleus to exert transcriptional comodulator activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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MAB2097
Smad4 Antibody (253343) [Unconjugated]
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Publications for Smad4 Antibody (MAB2097)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: CyTof.


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Bioinformatics

Gene Symbol SMAD4
Uniprot