Smad4 Antibodies
We offer Smad4 Antibodies for use in common research applications: Chromatin Immunoprecipitation (ChIP), ELISA, Flow (Intracellular), Flow Cytometry, Immunocytochemistry, Immunocytochemistry/Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Intracellular Staining by Flow Cytometry, Proximity Ligation Assay, Sandwich ELISA, Western Blot. Each Smad4 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Smad4 Antibodies can be used in a variety of model species: Human, Monkey, Mouse, Rat. Use the list below to choose the Smad4 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Smad4 polyclonal antibodies and browse our Smad4 monoclonal antibody catalog.
Alternate Names for Smad4 Antibodies
anti-Smad4 antibody, anti-DPC4SMAD, mothers against DPP homolog 4 (Drosophila) antibody, anti-hSMAD4 antibody, anti-JIP antibody, anti-MAD homolog 4 antibody, anti-MADH4mothers against decapentaplegic homolog 4 antibody, anti-mothers against decapentaplegic homolog 4 antibody, anti-mothers against decapentaplegic, Drosophila, homolog of, 4 antibody, anti-SMAD 4 antibody, anti-SMAD family member 4MAD, mothers against decapentaplegic homolog 4 (Drosophila) antibody, anti-SMAD, mothers against DPP homolog 4 antibody
27 Results for "smad4" in Primary Antibodies
![Western Blot: Smad4 Antibody [NBP2-24951] - SMAD4 Antibody [NBP2-24951] - Analysis using SMAD4 antibody. Lysate from A549 cells probed with SMAD4 antibody at 2 ug/mL.](http://images.novusbio.com/fullsize/SMAD4-Antibody-Western-Blot-NBP2-24951-img0001.jpg "Western Blot: Smad4 Antibody [NBP2-24951] - SMAD4 Antibody [NBP2-24951] - Analysis using SMAD4 antibody. Lysate from A549 cells probed with SMAD4 antibody at 2 ug/mL.")
![Immunocytochemistry/Immunofluorescence: Smad4 Antibody [NBP2-24951] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-SMAD4 Antibody NBP2-24951 at 2 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Smad4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-24951-img0003.jpg)
![Immunohistochemistry-Paraffin: Smad4 Antibody [NBP2-24951] - Analysis of a FFPE tissue section of human colon using 1:200 dilution of SMAD4 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. Apical cytoplasmic staining was observed in both glandular and epithelial cells.](http://images.novusbio.com/fullsize/Smad4-Antibody-Immunohistochemistry-Paraffin-NBP2-24951-img0002.jpg)
![Western Blot: Smad4 Antibody (1H7D3) [NBP3-15673] - Analysis of extracts of various cell lines, using Smad4 antibody (NBP3-15673) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Smad4-Antibody-1H7D3-Western-Blot-NBP3-15673-img0004.jpg "Western Blot: Smad4 Antibody (1H7D3) [NBP3-15673] - Analysis of extracts of various cell lines, using Smad4 antibody (NBP3-15673) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunohistochemistry-Paraffin: Smad4 Antibody (1H7D3) [NBP3-15673] - Human liver cancer using [KO Validated] Smad4 Rabbit mAb (NBP3-15673) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Smad4-Antibody-1H7D3-Immunohistochemistry-Paraffin-NBP3-15673-img0002.jpg)
![Western Blot: Smad4 Antibody (1H7D3) [NBP3-15673] - Analysis of extracts of various cell lines, using Smad4 antibody (NBP3-15673) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Smad4-Antibody-1H7D3-Western-Blot-NBP3-15673-img0003.jpg)
![<b>Genetic Strategies Validation. </b>Knockout Validated: Smad4 Antibody (1H7D3) [NBP3-15673] - Western blot analysis of extracts from normal (control) and Smad4 knockout (KO) 293T cells, using Smad4 antibody (NBP3-15673) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Smad4-Antibody-1H7D3-Knockout-Validated-NBP3-15673-img0001.jpg)
![Immunoprecipitation: Smad4 Antibody (1H7D3) [NBP3-15673] - Analysis of 300ug extracts of 293T cells using 3ug Smad4 antibody (NBP3-15673). Western blot was performed from the immunoprecipitate using Smad4 antibody (NBP3-15673) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/Smad4-Antibody-1H7D3-Immunoprecipitation-NBP3-15673-img0005.jpg)
![Western Blot: Smad4 Antibody (CL9774) [NBP3-05507] - Analysis in human cell line SCLC-21H.](http://images.novusbio.com/fullsize/Smad4-Antibody-CL9774-Western-Blot-NBP3-05507-img0006.jpg "Western Blot: Smad4 Antibody (CL9774) [NBP3-05507] - Analysis in human cell line SCLC-21H.")
![Immunohistochemistry-Paraffin: Smad4 Antibody (CL9774) [NBP3-05507] - Staining of human testis shows moderate nuclear and cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Smad4-Antibody-CL9774-Immunohistochemistry-Paraffin-NBP3-05507-img0005.jpg)
![Immunohistochemistry-Paraffin: Smad4 Antibody (CL9774) [NBP3-05507] - Staining of human placenta shows moderate nuclear and cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Smad4-Antibody-CL9774-Immunohistochemistry-Paraffin-NBP3-05507-img0001.jpg)
![Immunohistochemistry-Paraffin: Smad4 Antibody (CL9774) [NBP3-05507] - Staining of human prostate shows moderate nuclear and cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Smad4-Antibody-CL9774-Immunohistochemistry-Paraffin-NBP3-05507-img0002.jpg)
![Immunohistochemistry-Paraffin: Smad4 Antibody (CL9774) [NBP3-05507] - Staining of human skeletal muscle shows very weak cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/Smad4-Antibody-CL9774-Immunohistochemistry-Paraffin-NBP3-05507-img0003.jpg)
![Immunohistochemistry-Paraffin: Smad4 Antibody (CL9774) [NBP3-05507] - Staining of human stomach cancer shows strong cytoplasmic and nuclear positivity.](http://images.novusbio.com/fullsize/Smad4-Antibody-CL9774-Immunohistochemistry-Paraffin-NBP3-05507-img0004.jpg)
![Western Blot: Smad4 Antibody (4G1C6) [NBP1-51646] - Analysis using SMAD4 mouse mAb against A431 (1), SK-N-SH (2), K562 (3), HepG2 (4) and HUVE12 (5) cell lysate.](http://images.novusbio.com/fullsize/Smad4-Antibody-4G1C6-Western-Blot-NBP1-51646-img0015.jpg "Western Blot: Smad4 Antibody (4G1C6) [NBP1-51646] - Analysis using SMAD4 mouse mAb against A431 (1), SK-N-SH (2), K562 (3), HepG2 (4) and HUVE12 (5) cell lysate.")
![Immunocytochemistry/Immunofluorescence: Smad4 Antibody (4G1C6) [NBP1-51646] - Analysis of NIH/3T3 cells using SMAD4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/Smad4-Antibody-4G1C6-Immunocytochemistry-NBP1-51646-img0012.jpg)
![Immunohistochemistry-Paraffin: Smad4 Antibody (4G1C6) [NBP1-51646] - Analysis of lung cancer tissues using SMAD4 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Smad4-Antibody-4G1C6-Immunohistochemistry-Paraffin-NBP1-51646-img0014.jpg)
![Flow Cytometry: Smad4 Antibody (4G1C6) [NBP1-51646] - Analysis of K562 cells using SMAD4 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/Smad4-Antibody-4G1C6-Flow-Cytometry-NBP1-51646-img0009.jpg)
![Western Blot: Smad4 Antibody (PD01-37) [NBP3-32956] - Western blot analysis of Smad4 on different lysates with Rabbit anti-Smad4 antibody (NBP3-32956) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: Jurkat cell lysate Lane 3: SH-SY5Y cell lysate Lane 4: Ramos cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: PC-12 cell lysate Lane 7: C6 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 60 kDa Observed band size: 60 kDa Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32956) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32956_rabbit-smad4-mab-pd01-37-288202412461648.jpg "Western Blot: Smad4 Antibody (PD01-37) [NBP3-32956] - Western blot analysis of Smad4 on different lysates with Rabbit anti-Smad4 antibody (NBP3-32956) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: Jurkat cell lysate Lane 3: SH-SY5Y cell lysate Lane 4: Ramos cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: PC-12 cell lysate Lane 7: C6 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 60 kDa Observed band size: 60 kDa Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32956) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Smad4 Antibody (PD01-37) [NBP3-32956] - Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-Smad4 antibody (NBP3-32956) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32956) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32956_rabbit-smad4-mab-pd01-37-298202414304112.jpg)
![Immunocytochemistry/ Immunofluorescence: Smad4 Antibody (PD01-37) [NBP3-32956] - Immunocytochemistry analysis of PANC-1 cells labeling Smad4 with Rabbit anti-Smad4 antibody (NBP3-32956) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Smad4 antibody (NBP3-32956) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32956_rabbit-smad4-mab-pd01-37-29202410234283.jpg)
![Immunoprecipitation: Smad4 Antibody [NBP3-14714] - : Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from 293T cells preparedusing NETN lysis buffer. Antibodies: Rabbit anti-SMAD4recombinant monoclonal antibody used for IP at 6 ul per reaction. SMAD4 was alsoimmunoprecipitated by rabbit anti-SMAD4 antibody. For blotting immunoprecipitated SMAD4, was used at 1:1000. Chemiluminescence withan exposure time of 3 seconds.](http://images.novusbio.com/fullsize/Smad4-Antibody-Immunoprecipitation-NBP3-14714-img0001.jpg "Immunoprecipitation: Smad4 Antibody [NBP3-14714] - : Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from 293T cells preparedusing NETN lysis buffer. Antibodies: Rabbit anti-SMAD4recombinant monoclonal antibody used for IP at 6 ul per reaction. SMAD4 was alsoimmunoprecipitated by rabbit anti-SMAD4 antibody. For blotting immunoprecipitated SMAD4, was used at 1:1000. Chemiluminescence withan exposure time of 3 seconds.")
![Western Blot: Smad4 Antibody (RM277) [NBP2-61587] - Western Blot of Jurkat and IMR32 cells lysates using NBP2-61587.](http://images.novusbio.com/fullsize/Smad4-Antibody-RM277-Western-Blot-NBP2-61587-img0003.jpg "Western Blot: Smad4 Antibody (RM277) [NBP2-61587] - Western Blot of Jurkat and IMR32 cells lysates using NBP2-61587.")
![Immunohistochemistry-Paraffin: Smad4 Antibody (RM277) [NBP2-61587] - Immunohistochemical staining of formalin fixed and paraffin embedded Human breast cancer tissue sections using NBP2-61587.](http://images.novusbio.com/fullsize/Smad4-Antibody-RM277-Immunohistochemistry-Paraffin-NBP2-61587-img0001.jpg)
![Immunohistochemistry-Paraffin: Smad4 Antibody (RM277) [NBP2-61587] - Immunohistochemical staining of formalin fixed and paraffin embedded human colon cancer tissue sections using NBP2-61587 at a 1:5000 dilution.](http://images.novusbio.com/fullsize/nbp2-61587_rabbit-monoclonal-smad4-antibody-rm277-161020231947103.png)
![Immunohistochemistry-Paraffin: Smad4 Antibody (SMAD/6309R) [NBP3-13963] - Immunohistochemical stain using Smad4 antibody (SMAD/6309R) showing loss of SMAD4 expression in pancreatic ductal adenocarcinoma. Note adjacent benign ductal epithelium and background stroma as internal control showing positive / retained SMAD4 staining.](http://images.novusbio.com/fullsize/Smad4-Antibody-SMAD-6309R-Immunohistochemistry-Paraffin-NBP3-13963-img0001.jpg "Immunohistochemistry-Paraffin: Smad4 Antibody (SMAD/6309R) [NBP3-13963] - Immunohistochemical stain using Smad4 antibody (SMAD/6309R) showing loss of SMAD4 expression in pancreatic ductal adenocarcinoma. Note adjacent benign ductal epithelium and background stroma as internal control showing positive / retained SMAD4 staining.")
![Immunohistochemistry-Paraffin: Smad4 Antibody (SMAD/6309R) [NBP3-13963] - Formalin-fixed, paraffin-embedded human small intestine stained with Smad4 antibody (SMAD/6309R). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/Smad4-Antibody-SMAD-6309R-Immunohistochemistry-Paraffin-NBP3-13963-img0002.jpg)
![Immunohistochemistry-Paraffin: Smad4 Antibody (SMAD4/2440) [NBP3-07262] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Smad4 Mouse Monoclonal Antibody (Smad4/2440).](http://images.novusbio.com/fullsize/Smad4-Antibody-SMAD4-2440-Immunohistochemistry-Paraffin-NBP3-07262-img0002.jpg "Immunohistochemistry-Paraffin: Smad4 Antibody (SMAD4/2440) [NBP3-07262] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Smad4 Mouse Monoclonal Antibody (Smad4/2440).")
![Protein Array: Smad4 Antibody (SMAD4/2440) [NBP3-07262] - Analysis of Protein Array containing >19,000 full-length human proteins using Smad4 Mouse Monoclonal Antibody (Smad4/2440).](http://images.novusbio.com/fullsize/Smad4-Antibody-SMAD4-2440-Protein-Array-NBP3-07262-img0001.jpg)