![Immunohistochemistry: Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - Early social isolation (ESI) induces long-term changes in sirtuin1 (SIRT) mRNA expression in the mouse brain and blood and reduces the SIRT1 protein level in different brain regions. Confocal images from the hippocampus and basolateral amygdala of control and ESI mice reacted with SIRT1 antibody. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/tp2015125) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-1F3-Immunohistochemistry-NBP1-51641-img0015.jpg "Immunohistochemistry: Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - Early social isolation (ESI) induces long-term changes in sirtuin1 (SIRT) mRNA expression in the mouse brain and blood and reduces the SIRT1 protein level in different brain regions. Confocal images from the hippocampus and basolateral amygdala of control and ESI mice reacted with SIRT1 antibody. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/tp2015125) licensed under a CC-BY license.")
| Reactivity | Hu, Mu, Rt, Pm, RbSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, IHC, IHC |
| Clone | 1F3 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Immunogen | Purified recombinant fragment of human SIRT1 (amino acids: 265-452) expressed in E. coli. [UniProt# Q96EB6] |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | SIRT1 |
| Purity | Ammonium sulfate precipitation |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 120 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Ammonium sulfate precipitation |
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Secondary Antibodies |
Isotype Controls |
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![Western Blot: Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - Western blot analysis using SIRT1 mouse mAb against MCF-7 (1), Jurkat (2), Hela (3), HEK293 (4) and A549 (5) cell lysates.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-1F3-Western-Blot-NBP1-51641-img0007.jpg "Western Blot: Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - Western blot analysis using SIRT1 mouse mAb against MCF-7 (1), Jurkat (2), Hela (3), HEK293 (4) and A549 (5) cell lysates.")
![Immunocytochemistry/Immunofluorescence: Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - SIRT1 antibody was tested at 1:10 in Ntera2 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-1F3-Immunocytochemistry-Immunofluorescence-NBP1-51641-img0012.jpg "Immunocytochemistry/Immunofluorescence: Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - SIRT1 antibody was tested at 1:10 in Ntera2 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x.")
![Flow Cytometry: Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - Intracellular flow cytometric staining of 1 x 10^6 HEK-293 cells using SIRT1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-1F3-Flow-Cytometry-NBP1-51641-img0010.jpg "Flow Cytometry: Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - Intracellular flow cytometric staining of 1 x 10^6 HEK-293 cells using SIRT1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.")
![Immunocytochemistry/Immunofluorescence: Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - Analysis of NTERA-2 cells using SIRT1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-1F3-Immunocytochemistry-Immunofluorescence-NBP1-51641-img0006.jpg "Immunocytochemistry/Immunofluorescence: Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - Analysis of NTERA-2 cells using SIRT1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.")
![Immunohistochemistry-Paraffin: Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - Immunohistochemical analysis of paraffin-embedded lung cancer tissues (left) and kidney cancer tissues (right) using SIRT1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-1F3-Immunohistochemistry-Paraffin-NBP1-51641-img0003.jpg "Immunohistochemistry-Paraffin: Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - Immunohistochemical analysis of paraffin-embedded lung cancer tissues (left) and kidney cancer tissues (right) using SIRT1 mouse mAb with DAB staining.")
![Flow (Intracellular): Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - An intracellular stain was performed on RAW 246.7 cells with Sirtuin 1/SIRT1 Antibody (1F3) NBP1-51641APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-1F3-Flow-Intracellular-NBP1-51641-img0014.jpg "Flow (Intracellular): Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - An intracellular stain was performed on RAW 246.7 cells with Sirtuin 1/SIRT1 Antibody (1F3) NBP1-51641APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin.")
![Flow Cytometry: Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - Flow cytometric analysis of K562 cells using SIRT1 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-1F3-Flow-Cytometry-NBP1-51641-img0005.jpg "Flow Cytometry: Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - Flow cytometric analysis of K562 cells using SIRT1 mouse mAb (green) and negative control (purple).")
![Immunocytochemistry/ Immunofluorescence: Sirtuin 1/SIRT1 Antibody (1F3) - BSA Free [NBP1-51641] - Early social isolation (ESI) induces long-term changes in sirtuin1 (SIRT) mRNA expression in the mouse brain & blood & reduces the SIRT1 protein level in different brain regions. (a) The mRNA expression levels (relative expression levels=fold changes over control, normalized to PGK1 & TBP (Tata Binding Protein) genes) of SIRT1 & SIRT6 were significantly decreased in the brain of ESI compared with control mice. (b) In peripheral blood mononuclear cells (PBMCs) of ESI-treated mice, the expression level of SIRT1 mRNA was downregulated. ESI, n=9 (male (M)=6, female (F)=3); Control, n=7 (M=4, F=3) ***P<0.001; *P<0.05. (c) Confocal images showing SIRT1 (red) & NeuN (green) immunostaining plus 4,6-diamidino-2-phenylindole (DAPI) counterstaining (gray) in the brain of control mice. SIRT1 (red) colocalizes both with the neuronal marker NeuN (green) & with DAPI (gray). (d) Confocal images from the hippocampus & basolateral amygdala of control & ESI mice reacted with SIRT1 antibody. (e) Densitometric analysis of SIRT1 immunoreactivity in the motor cortex, striatum, hippocampus & basolateral amygdala revealed a significant reduction of SIRT1 immunoreactivity in ESI compared with control mice. The F/A ratio defines the mean fluorescence of individual samples (F) normalized to total surface (A). ESI, n=5; Control, n=5. ***P<0.001. Scale bars: (d) 100 μm; 25 μm. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/tp2015125), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-51641_mouse-monoclonal-sirtuin-1-sirt1-antibody-1f3-310202416165619.jpg "Immunocytochemistry/ Immunofluorescence: Sirtuin 1/SIRT1 Antibody (1F3) - BSA Free [NBP1-51641] - Early social isolation (ESI) induces long-term changes in sirtuin1 (SIRT) mRNA expression in the mouse brain & blood & reduces the SIRT1 protein level in different brain regions. (a) The mRNA expression levels (relative expression levels=fold changes over control, normalized to PGK1 & TBP (Tata Binding Protein) genes) of SIRT1 & SIRT6 were significantly decreased in the brain of ESI compared with control mice. (b) In peripheral blood mononuclear cells (PBMCs) of ESI-treated mice, the expression level of SIRT1 mRNA was downregulated. ESI, n=9 (male (M)=6, female (F)=3); Control, n=7 (M=4, F=3) ***P<0.001; *P<0.05. (c) Confocal images showing SIRT1 (red) & NeuN (green) immunostaining plus 4,6-diamidino-2-phenylindole (DAPI) counterstaining (gray) in the brain of control mice. SIRT1 (red) colocalizes both with the neuronal marker NeuN (green) & with DAPI (gray). (d) Confocal images from the hippocampus & basolateral amygdala of control & ESI mice reacted with SIRT1 antibody. (e) Densitometric analysis of SIRT1 immunoreactivity in the motor cortex, striatum, hippocampus & basolateral amygdala revealed a significant reduction of SIRT1 immunoreactivity in ESI compared with control mice. The F/A ratio defines the mean fluorescence of individual samples (F) normalized to total surface (A). ESI, n=5; Control, n=5. ***P<0.001. Scale bars: (d) 100 μm; 25 μm. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/tp2015125), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")