Sirtuin 1/SIRT1 Antibodies
We offer Sirtuin 1/SIRT1 Antibodies for use in common research applications: ELISA, Flow Cytometry, Immunocytochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Proximity Ligation Assay, Sandwich ELISA, Simple Western, Western Blot. Each Sirtuin 1/SIRT1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Sirtuin 1/SIRT1 Antibodies can be used in a variety of model species: Amphibian, Bovine, Canine, Chicken, Chinese Hamster, Equine, Feline, Human, Mouse, Porcine, Primate, Rabbit, Rat. Use the list below to choose the Sirtuin 1/SIRT1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Sirtuin 1/SIRT1 polyclonal antibodies and browse our Sirtuin 1/SIRT1 monoclonal antibody catalog.
Alternate Names for Sirtuin 1/SIRT1 Antibodies
anti-Sirtuin 1/SIRT1 antibody, anti-SIRT1 antibody, anti-EC 3.5.1 antibody, anti-S. cerevisiae, homolog) 1 antibody, anti-sirtuin 1 antibody
16 Results for "sirtuin-1-sirt1" in Primary Antibodies
![Immunohistochemistry: Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - Early social isolation (ESI) induces long-term changes in sirtuin1 (SIRT) mRNA expression in the mouse brain and blood and reduces the SIRT1 protein level in different brain regions. Confocal images from the hippocampus and basolateral amygdala of control and ESI mice reacted with SIRT1 antibody. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/tp2015125) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-1F3-Immunohistochemistry-NBP1-51641-img0015.jpg "Immunohistochemistry: Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - Early social isolation (ESI) induces long-term changes in sirtuin1 (SIRT) mRNA expression in the mouse brain and blood and reduces the SIRT1 protein level in different brain regions. Confocal images from the hippocampus and basolateral amygdala of control and ESI mice reacted with SIRT1 antibody. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/tp2015125) licensed under a CC-BY license.")
![Western Blot: Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - Western blot analysis using SIRT1 mouse mAb against MCF-7 (1), Jurkat (2), Hela (3), HEK293 (4) and A549 (5) cell lysates.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-1F3-Western-Blot-NBP1-51641-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - SIRT1 antibody was tested at 1:10 in Ntera2 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-1F3-Immunocytochemistry-Immunofluorescence-NBP1-51641-img0012.jpg)
![Flow Cytometry: Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - Intracellular flow cytometric staining of 1 x 10^6 HEK-293 cells using SIRT1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-1F3-Flow-Cytometry-NBP1-51641-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - Analysis of NTERA-2 cells using SIRT1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-1F3-Immunocytochemistry-Immunofluorescence-NBP1-51641-img0006.jpg)
![Immunohistochemistry-Paraffin: Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - Immunohistochemical analysis of paraffin-embedded lung cancer tissues (left) and kidney cancer tissues (right) using SIRT1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-1F3-Immunohistochemistry-Paraffin-NBP1-51641-img0003.jpg)
![Flow (Intracellular): Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - An intracellular stain was performed on RAW 246.7 cells with Sirtuin 1/SIRT1 Antibody (1F3) NBP1-51641APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-1F3-Flow-Intracellular-NBP1-51641-img0014.jpg)
![Flow Cytometry: Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - Flow cytometric analysis of K562 cells using SIRT1 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-1F3-Flow-Cytometry-NBP1-51641-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: Sirtuin 1/SIRT1 Antibody (1F3) - BSA Free [NBP1-51641] - Early social isolation (ESI) induces long-term changes in sirtuin1 (SIRT) mRNA expression in the mouse brain & blood & reduces the SIRT1 protein level in different brain regions. (a) The mRNA expression levels (relative expression levels=fold changes over control, normalized to PGK1 & TBP (Tata Binding Protein) genes) of SIRT1 & SIRT6 were significantly decreased in the brain of ESI compared with control mice. (b) In peripheral blood mononuclear cells (PBMCs) of ESI-treated mice, the expression level of SIRT1 mRNA was downregulated. ESI, n=9 (male (M)=6, female (F)=3); Control, n=7 (M=4, F=3) ***P<0.001; *P<0.05. (c) Confocal images showing SIRT1 (red) & NeuN (green) immunostaining plus 4,6-diamidino-2-phenylindole (DAPI) counterstaining (gray) in the brain of control mice. SIRT1 (red) colocalizes both with the neuronal marker NeuN (green) & with DAPI (gray). (d) Confocal images from the hippocampus & basolateral amygdala of control & ESI mice reacted with SIRT1 antibody. (e) Densitometric analysis of SIRT1 immunoreactivity in the motor cortex, striatum, hippocampus & basolateral amygdala revealed a significant reduction of SIRT1 immunoreactivity in ESI compared with control mice. The F/A ratio defines the mean fluorescence of individual samples (F) normalized to total surface (A). ESI, n=5; Control, n=5. ***P<0.001. Scale bars: (d) 100 μm; 25 μm. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/tp2015125), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-51641_mouse-monoclonal-sirtuin-1-sirt1-antibody-1f3-310202416165619.jpg)
![Western Blot: Sirtuin 1/SIRT1 Antibody [NBP2-27205] - Analysis of SIRT1 using SIRT1 antibody. HeLa lysate probed with 1) prebleed, 2) SIRT1 antibody and 3) mouse NIH 3T3 lysate probed with SIRT1 antibody at2 ug/ml. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-Western-Blot-NBP2-27205-img0003.jpg "Western Blot: Sirtuin 1/SIRT1 Antibody [NBP2-27205] - Analysis of SIRT1 using SIRT1 antibody. HeLa lysate probed with 1) prebleed, 2) SIRT1 antibody and 3) mouse NIH 3T3 lysate probed with SIRT1 antibody at2 ug/ml. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Simple Western: Sirtuin 1/SIRT1 Antibody [NBP2-27205] - Simple Western lane view shows a specific band for Sirtuin 1/SIRT1 in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-Simple-Western-NBP2-27205-img0002.jpg)
![Western Blot: Sirtuin 1/SIRT1 Antibody [NBP1-49540] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and SIRT1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human SIRT1 Polyclonal Antibody (Catalog # NBP1-49540) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for SIRT1 at approximately 130 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-Knockdown-Validated-NBP1-49540-img0007.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Sirtuin 1/SIRT1 Antibody [NBP1-49540] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and SIRT1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human SIRT1 Polyclonal Antibody (Catalog # NBP1-49540) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for SIRT1 at approximately 130 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: Sirtuin 1/SIRT1 Antibody [NBP1-49540] - Analysis of SIRT1 in HeLa whole cell lyates.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-Western-Blot-NBP1-49540-img0004.jpg)
![Immunohistochemistry: Sirtuin 1/SIRT1 Antibody [NBP1-49540] - Immunohistochemical analysis of SIRT1 in human renal cell carcinoma.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-Immunohistochemistry-NBP1-49540-img0005.jpg)
![Simple Western: Sirtuin 1/SIRT1 Antibody [NBP1-49540] - Simple Western lane view shows a specific band for SIRT1 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-Simple-Western-NBP1-49540-img0006.jpg)
![Western Blot: Sirtuin 1/SIRT1 Antibody (7B7) [H00023411-M01] - SIRT1 monoclonal antibody (M01), clone 7B7 Analysis of SIRT1 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-7B7-Western-Blot-H00023411-M01-img0014.jpg "Western Blot: Sirtuin 1/SIRT1 Antibody (7B7) [H00023411-M01] - SIRT1 monoclonal antibody (M01), clone 7B7 Analysis of SIRT1 expression in Hela S3 NE.")
![Immunocytochemistry/Immunofluorescence: Sirtuin 1/SIRT1 Antibody (7B7) [H00023411-M01] - Analysis of monoclonal antibody to SIRT1 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-7B7-Immunocytochemistry-Immunofluorescence-H00023411-M01-img0010.jpg)
![Immunohistochemistry-Paraffin: Sirtuin 1/SIRT1 Antibody (7B7) [H00023411-M01] - Analysis of monoclonal antibody to SIRT1 on formalin-fixed paraffin-embedded human lateral ventricle wall. Antibody concentration 1 ug/ml.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-7B7-Immunohistochemistry-Paraffin-H00023411-M01-img0012.jpg)
![Sandwich ELISA: Sirtuin 1/SIRT1 Antibody (7B7) [H00023411-M01] - Detection limit for recombinant GST tagged SIRT1 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-7B7-Sandwich-ELISA-H00023411-M01-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: Sirtuin 1/SIRT1 Antibody [NBP1-87038] - Staining of human cell line U-251 MG shows localization to nucleus & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87038-img0004.jpg "Immunocytochemistry/Immunofluorescence: Sirtuin 1/SIRT1 Antibody [NBP1-87038] - Staining of human cell line U-251 MG shows localization to nucleus & cytosol. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: Sirtuin 1/SIRT1 Antibody [NBP1-87038] - Staining of human lymph node shows moderate to strong nuclear positivity in germinal center cells.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-Immunohistochemistry-Paraffin-NBP1-87038-img0009.jpg)
![Immunohistochemistry-Paraffin: Sirtuin 1/SIRT1 Antibody [NBP1-87038] - Saining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-Immunohistochemistry-Paraffin-NBP1-87038-img0006.jpg)
![Immunohistochemistry-Paraffin: Sirtuin 1/SIRT1 Antibody [NBP1-87038] - Staining of human placenta shows strong nuclear positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-Immunohistochemistry-Paraffin-NBP1-87038-img0007.jpg)
![Immunohistochemistry-Paraffin: Sirtuin 1/SIRT1 Antibody [NBP1-87038] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-Immunohistochemistry-Paraffin-NBP1-87038-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Sirtuin 1/SIRT1 Antibody (PCRP-SIRT1-1E11) [NBP3-13916] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using Sirtuin 1/SIRT1 Antibody (PCRP-SIRT1-1E11) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-PCRP-SIRT1-1E11-Immunocytochemistry-Immunofluorescence-NBP3-13916-img0002.jpg "Immunocytochemistry/Immunofluorescence: Sirtuin 1/SIRT1 Antibody (PCRP-SIRT1-1E11) [NBP3-13916] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using Sirtuin 1/SIRT1 Antibody (PCRP-SIRT1-1E11) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).")
![Protein Array: Sirtuin 1/SIRT1 Antibody (PCRP-SIRT1-1E11) [NBP3-13916] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Sirtuin 1/SIRT1 Antibody (PCRP-SIRT1-1E11).](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-PCRP-SIRT1-1E11-Protein-Array-NBP3-13916-img0004.jpg)
![Western Blot: Sirtuin 1/SIRT1 [p Ser 47] Antibody (SR1063) [NBP3-21546] - Western blot analysis using NBP3-21546 in (1) HEK293 cell lysate; (2) HEK293 cell lysate treated with LP.](http://images.novusbio.com/fullsize/nbp3-21546_rabbit-sirtuin-1-sirt1-p-ser-47-mab-sr1063-672023106236.jpg "Western Blot: Sirtuin 1/SIRT1 [p Ser 47] Antibody (SR1063) [NBP3-21546] - Western blot analysis using NBP3-21546 in (1) HEK293 cell lysate; (2) HEK293 cell lysate treated with LP.")
![Immunohistochemistry: Sirtuin 1/SIRT1 [p Ser 47] Antibody (SR1063) [NBP3-21546] - Immunohistochemical analysis of paraffin-embedded human colon, using NBP3-21546.](http://images.novusbio.com/fullsize/nbp3-21546_rabbit-sirtuin-1-sirt1-p-ser-47-mab-sr1063-57202316112183.jpg)
![Immunohistochemistry: Sirtuin 1/SIRT1 Antibody (9E9) [NBP3-26324] - Image of Sirtuin 1/SIRT1 Antibody (9E9) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26324_rabbit-sirtuin-1-sirt1-mab-9e9-2722024827498.jpg "Immunohistochemistry: Sirtuin 1/SIRT1 Antibody (9E9) [NBP3-26324] - Image of Sirtuin 1/SIRT1 Antibody (9E9) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunocytochemistry/Immunofluorescence: Sirtuin 1/SIRT1 Antibody (9E9) [NBP3-26324] - Staining of Hela Cells with Sirtuin 1/SIRT1 Antibody (9E9) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26324_rabbit-sirtuin-1-sirt1-mab-9e9-28220241323628.jpg)
![Western Blot: Sirtuin 1/SIRT1 Antibody (S06-4K9) [NBP3-14881] - Western blot detection of Sirtuin 1/SIRT1 in K562, C6, 3T3, Hela cell lysates using Sirtuin 1/SIRT1 Rabbit mAb (1:1000 diluted). Predicted band size: 82kDa. Observed band size: 120kDa.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-S06-4K9-Western-Blot-NBP3-14881-img0001.jpg "Western Blot: Sirtuin 1/SIRT1 Antibody (S06-4K9) [NBP3-14881] - Western blot detection of Sirtuin 1/SIRT1 in K562, C6, 3T3, Hela cell lysates using Sirtuin 1/SIRT1 Rabbit mAb (1:1000 diluted). Predicted band size: 82kDa. Observed band size: 120kDa.")
![Immunocytochemistry/Immunofluorescence: Sirtuin 1/SIRT1 Antibody [NBP2-56849] - Staining of human cell line U-2 OS shows localization to nucleoplasm & mitochondria.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56849-img0001.jpg "Immunocytochemistry/Immunofluorescence: Sirtuin 1/SIRT1 Antibody [NBP2-56849] - Staining of human cell line U-2 OS shows localization to nucleoplasm & mitochondria.")