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Sirtuin 1/SIRT1 [p Ser47] Antibody (SR1063)

Images

 
Western Blot: Sirtuin 1/SIRT1 [p Ser 47] Antibody (SR1063) [NBP3-21546] - Western blot analysis using NBP3-21546 in (1) HEK293 cell lysate; (2) HEK293 cell lysate treated with LP.
Immunohistochemistry: Sirtuin 1/SIRT1 [p Ser 47] Antibody (SR1063) [NBP3-21546] - Immunohistochemical analysis of paraffin-embedded human colon, using NBP3-21546.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
SR1063
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Sirtuin 1/SIRT1 [p Ser47] Antibody (SR1063) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
A synthesized peptide derived from human phospho-Sirtuin 1/SIRT1 [Ser47] (Uniprot #: Q96EB6)
Modification
p Ser47
Specificity
Detects endogenous levels of phospho-Sirtuin 1/SIRT1 [Ser47]
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
SIRT1
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:1000-1:2000
Theoretical MW
110 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.4, 150mM NaCl, 50% glycerol.
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for Sirtuin 1/SIRT1 [p Ser47] Antibody (SR1063)

  • EC 3.5.1
  • S. cerevisiae, homolog) 1
  • SIR2alpha
  • SIR2L1
  • SIR2-like protein 1
  • SIRT1
  • Sirtuin 1

Background

The S. cerevisiae silent information regulator 2 (Sir2) protein is an NAD+-dependent histone deacetylase (HDACs) that plays a critical role in transcriptional silencing, genome stability, and longevity (1-2). Part of the Sirtuin family (Sir2, Sir3, Sir3), yeast Sir2 is the founding member of Class III HDACs. A human homologue of Sir2 , SIRT1, regulates the activity of the p53 tumor suppressor and inhibits apoptosis (3-4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol SIRT1