Western Blot: PBEF/Visfatin/NAMPT Antibody [NB100-594] - Samples: Whole cell lysate (50 ug) from HeLa, RKO, MCF-7, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PBEF ...read more
Immunohistochemistry-Paraffin: PBEF/Visfatin/NAMPT Antibody [NB100-594] - Section of human head and neck squamous cell carcinoma. Antibody: Affinity purified rabbit anti-PBEF used at a dilution of 1:5,000 (0.2ug/ml). ...read more
Western Blot: PBEF/Visfatin/NAMPT Antibody [NB100-594] - Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit ...read more
The immunogen recognized by this antibody maps to a region between residues 400 and 450 of human pre-B-cell colony enhancing factor 1 using the numbering given in Swiss-Prot entry P43490 (GeneID 10135).
Predicted Species
Rat (100%), Porcine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NAMPT
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Epitope retrieval with Citrate buffer pH6.0 is recommended for FFPE tissue sections.
Theoretical MW
56 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using NB100-594 in the following applications:
The recently discovered adipocytokine PBEF is a visceral fat specific protein which has been shown to exert insulin-mimetic effects in insulin-sensitive tissues such as liver, muscle and fat. PBEF can bind to the insulin receptor and PBEF plasma levels increase with obesity. Based on their properties, adipocytokines are likely to play critical roles in metabolic syndromes such as obesity and diabetes.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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