![Immunocytochemistry/Immunofluorescence: Sirtuin 1/SIRT1 Antibody (PCRP-SIRT1-1E11) [NBP3-13916] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using Sirtuin 1/SIRT1 Antibody (PCRP-SIRT1-1E11) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-PCRP-SIRT1-1E11-Immunocytochemistry-Immunofluorescence-NBP3-13916-img0002.jpg "Immunocytochemistry/Immunofluorescence: Sirtuin 1/SIRT1 Antibody (PCRP-SIRT1-1E11) [NBP3-13916] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using Sirtuin 1/SIRT1 Antibody (PCRP-SIRT1-1E11) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, IP, MA |
| Clone | PCRP-SIRT1-1E11 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Description | 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14290) Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. |
| Immunogen | Recombinant full-length human Sirtuin 1/SIRT1protein (Uniprot: Q96EB6) |
| Localization | Cytoplasm, Mitochondrion and Nucleus, PML body |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | SIRT1 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Application Notes | ELISA: For coating, order antibody without BSA Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate) |
| Theoretical MW | 120 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A or G purified |
![Western Blot AKT [p Ser473] Antibody [Unconjugated] - Pan Specific](https://images.novusbio.com/images2/Akt3_AF887_Western_Blot_5930.jpg)
![Simple Western AKT [p Ser473] Antibody [Unconjugated] - Pan Specific](https://images.novusbio.com/images2/16332.jpg)
![Intracellular Staining by Flow Cytometry AKT [p Ser473] Antibody [Unconjugated] - Pan Specific](https://images.novusbio.com/images2/Akt3_AF887_Flow_Cytometry_8283.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for Sirtuin 1/SIRT1 Antibody (NBP3-13916)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Protein Array: Sirtuin 1/SIRT1 Antibody (PCRP-SIRT1-1E11) [NBP3-13916] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Sirtuin 1/SIRT1 Antibody (PCRP-SIRT1-1E11).](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-PCRP-SIRT1-1E11-Protein-Array-NBP3-13916-img0004.jpg "Protein Array: Sirtuin 1/SIRT1 Antibody (PCRP-SIRT1-1E11) [NBP3-13916] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Sirtuin 1/SIRT1 Antibody (PCRP-SIRT1-1E11).")
![Immunocytochemistry Insulin Antibody (182410) [Unconjugated]](https://images.novusbio.com/images2/Insulin_MAB1417_Immunocytochemistry_9376.jpg)
![Immunohistochemistry Insulin Antibody (182410) [Unconjugated]](https://images.novusbio.com/images2/mab1417_human-bovine-mouse-insulin-mab-clone-182410-immunohistochemistry-308202115145.jpg)
![Western Blot AMPK alpha 2 Antibody [Unconjugated]](https://images.novusbio.com/images2/AMPK_alpha_2_AF2850_Western_Blot_5219.jpg)
![Simple Western AMPK alpha 2 Antibody [Unconjugated]](https://images.novusbio.com/images2/AMPK_alpha_2_AF2850_Simple_Western_16678.jpg)
![Immunocytochemistry AMPK alpha 2 Antibody [Unconjugated]](https://images.novusbio.com/images2/AMPK_alpha_2_AF2850_Immunocytochemistry__Immunofluorescence_19686.jpg)
![Western Blot AMPK beta 1 Antibody [Unconjugated]](https://images.novusbio.com/images2/AMPK_beta_1_AF2854_Western_Blot_5220.jpg)
![Immunohistochemistry AMPK beta 1 Antibody [Unconjugated]](https://images.novusbio.com/images/af2854_human-mouse-rat-ampk-beta-1-affinity-purified-polyclonal-ab-immunohistochemistry-252023124938.jpg)
![Immunohistochemistry AMPK beta 1 Antibody [Unconjugated]](https://images.novusbio.com/images/af2854_human-mouse-rat-ampk-beta-1-affinity-purified-polyclonal-ab-immunohistochemistry-252023125022.jpg)
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")