Western Blot: SENP1 Antibody [NB100-56405] - analysis of SENP1 in A) Daudi, B) HeLa and C) NIH 3T3 cell lysate using this antibody.
Simple Western: SENP1 Antibody [NB100-56405] - Simple Western lane view shows a specific band for SENP1 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more
Immunocytochemistry/ Immunofluorescence reported in scientific literature (PMID 27882949)
Immunohistochemistry
Immunohistochemistry-Paraffin reported in scientific literature (PMID 27882949)
Simple Western 1:100
Western Blot 3 - 5 ug/ml
Application Notes
A 66 kDa band is observed. In human and mouse, SENP1 has different isoforms between 664 and 699 amino acids long. Additional bands seen in western blot may represent other SUMOlated proteins. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size.
Covalent attachment of one protein to another is one of the more prominent posttranslational modifications in respect to size and ubiquity. Ubiquitin is the most familiar of the protein modifiers and its activation and transfer to target proteins has been studied extensively. Recently, a new group of ubiquitin-like proteins has been receiving a lot of attention. SUMO, or Sentrin, is one of the most intriguing. SUMO has been implicated in the stabilization of target proteins and/or their localization to sub-cellular complexes.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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