![Western Blot: NIH 3T3 Whole Cell Lysate [NB800-PC8] - Analysis of GAPDH (NB300-323) using NIH3T3 whole cell lysate [NB800-PC8].](http://images.novusbio.com/fullsize/NIH-3T3-Whole-Cell-Lysate-Western-Blot-NB800-PC8-img0004.jpg "Western Blot: NIH 3T3 Whole Cell Lysate [NB800-PC8] - Analysis of GAPDH (NB300-323) using NIH3T3 whole cell lysate [NB800-PC8].")
| Reactivity | MuSpecies Glossary |
| Applications | WB, PAGE |
| Concentration | 2.5 mg/ml |
| Dilutions |
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| Application Notes | This lysate is prepared as a positive control for separation by SDS-PAGE and subsequent Western blot analysis. Lysates are prepared in denaturing buffer WITHOUT reducing agents (i.e. 2-mercaptoethanol (BME) or dithiothreitol (DTT)). If reducing conditions are desired, add a reducing agent prior to heating. Heat lysate to 95 degrees C for 5 minutes and rapidly cool. The recommended loading amount is 0.01-0.03 mg per lane. |
| Storage | Store at -80C. Avoid freeze-thaw cycles. |
| Buffer | Standard RIPA buffer including protease and phosphatase inhibitors and 0.1% SDS diluted in 1 X lithium dodecyl sulfate (LDS) sample buffer with bromophenol blue and 40-70% glycerol, pH 8.4. BME has not been added. |
| Preservative | No Preservative |
| Concentration | 2.5 mg/ml |
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![SDS-Page: NIH 3T3 Whole Cell Lysate [NB800-PC8] - Ponceau S staining of NIH 3T3 whole cell lysate using NB800-PC8.](http://images.novusbio.com/fullsize/NIH-3T3-Whole-Cell-Lysate-SDS-Page-NB800-PC8-img0005.jpg "SDS-Page: NIH 3T3 Whole Cell Lysate [NB800-PC8] - Ponceau S staining of NIH 3T3 whole cell lysate using NB800-PC8.")