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SUMO2 Antibody (SUMO2/1199)

Images

 
Immunohistochemistry-Paraffin: SUMO2 Antibody (SUMO2/1199) [NBP2-44477] - Rat Ovary stained with SUMO-2 Monoclonal Antibody (SUMO2/1199)
Immunohistochemistry-Paraffin: SUMO2 Antibody (SUMO2/1199) [NBP2-44477] - Human Ovarian Carcinoma stained with SUMO-2 Monoclonal Antibody (SUMO2/1199)
Immunohistochemistry-Paraffin: SUMO2 Antibody (SUMO2/1199) [NBP2-44477] - Human Testicular Carcinoma stained with SUMO-2 Monoclonal Antibody (SUMO2/1199)
Immunohistochemistry-Paraffin: SUMO2 Antibody (SUMO2/1199) [NBP2-44477] - Human Tonsil stained with SUMO-2 Monoclonal Antibody (SUMO2/1199)
Immunohistochemistry-Paraffin: SUMO2 Antibody (SUMO2/1199) [NBP2-44477] - Human Tonsil stained with SUMO-2 Monoclonal Antibody (SUMO2/1199)
Immunohistochemistry-Paraffin: SUMO2 Antibody (SUMO2/1199) [NBP2-44477] - Rat Heart stained with SUMO-2 Monoclonal Antibody (SUMO2/1199)

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications Flow, ICC/IF, IHC, ICC/IF
Clone
SUMO2/1199
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

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SUMO2 Antibody (SUMO2/1199) Summary

Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47710)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Immunogen
Recombinant human SUMO2 protein (Uniprot: P55854)
Localization
Nuclear
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
SUMO2
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunofluorescence 0.5 - 1.0 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Publications
Read Publication using
NBP2-44477 in the following applications:

  • ICC
    1 publication

Reactivity Notes

Predicted to show a broad species reactivity.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for SUMO2 Antibody (SUMO2/1199)

  • HSMT3
  • MGC117191
  • sentrin 2
  • Sentrin-2
  • small ubiquitin-like modifier 2
  • small ubiquitin-related modifier 2
  • SMT3 (suppressor of mif two 3, yeast) homolog 2
  • SMT3 homolog 2
  • SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae)
  • SMT3 suppressor of mif two 3 homolog 2 (yeast)
  • SMT3A
  • SMT3B
  • SMT3H2
  • SUMO2
  • SUMO-2
  • SUMO3
  • SUMO-3
  • Ubiquitin-like protein SMT3A
  • ubiquitin-like protein SMT3B

Background

Function: Ubiquitin-like protein which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Does not seem to be involved in protein degradation and may function as an antagonist of ubiquitin in the degradation process. Plays a role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Covalent attachment to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4; Subcellular location: Nucleus

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SUMO2 Antibody (NBP2-44477)(1)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 1 application: ICC.


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ICC
(1)
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Filter By Species
Rat
(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for SUMO2 Antibody (NBP2-44477) (0)

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Secondary Antibodies

 

Isotype Controls

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Research Areas for SUMO2 Antibody (NBP2-44477)

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Bioinformatics

Gene Symbol SUMO2
Uniprot