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PIAS1 Antibody - BSA Free

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Western Blot: PIAS1 Antibody [NBP1-77158] - human kidney tissue lysate with PIAS1 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.
Western Blot: PIAS1 Antibody [NBP1-77158] - human kidney tissue lysate with PIAS1 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry: PIAS1 Antibody [NBP1-77158] - Rat kidney tissue at 20 ug/mL.
Immunohistochemistry-Paraffin: PIAS1 Antibody [NBP1-77158] - Rat kidney tissue with PIAS1 antibody at 5 ug/mL.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-77158AF350
Alexa Fluor 405
NBP1-77158AF405
Alexa Fluor 488
NBP1-77158AF488
Alexa Fluor 532
NBP1-77158AF532
Alexa Fluor 594
NBP1-77158AF594
Alexa Fluor 647
NBP1-77158AF647
Alexa Fluor 700
NBP1-77158AF700
Alexa Fluor 750
NBP1-77158AF750
Biotin
NBP1-77158B
DyLight 350
NBP1-77158UV
DyLight 405
NBP1-77158V
DyLight 488
NBP1-77158G
DyLight 550
NBP1-77158R
DyLight 594
NBP1-77158DL594
DyLight 650
NBP1-77158C
DyLight 680
NBP1-77158FR
DyLight 755
NBP1-77158IR
FITC
NBP1-77158F
HRP
NBP1-77158H
Janelia Fluor 549
NBP1-77158JF549
Janelia Fluor 646
NBP1-77158JF646
PerCP
NBP1-77158PCP
mFluor Violet 450 SE
NBP1-77158MFV450
mFluor Violet 500 SE
NBP1-77158MFV500
mFluor Violet 610 SE
NBP1-77158MFV610
View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NBP1-77158-0.025mg
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PIAS1 Antibody - BSA Free Summary

Immunogen
Antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human PIAS1. The immunogen is located within the last 50 amino acids of PIAS1. Amino Acid Squence: SNSLRESHSHTVTNR
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PIAS1
Purity
Peptide affinity purified
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Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 1 ug/ml
Control Peptide
PIAS1 Peptide (NBP1-77158PEP)
Reviewed Applications
Read 1 Review rated 3
using
NBP1-77158 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for PIAS1 Antibody - BSA Free

  • AR interacting protein
  • DDXBP1
  • DDXBP1MGC141878
  • DEAD/H (Asp-Glu-Ala-Asp/His) box binding protein 1
  • DEAD/H box-binding protein 1
  • E3 SUMO-protein ligase PIAS1
  • GBPRNA helicase II-binding protein
  • GU/RH-II
  • Gu-binding protein
  • MGC141879
  • PIAS1
  • Protein inhibitor of activated STAT protein 1
  • protein inhibitor of activated STAT, 1
  • protein inhibitor of activated STAT-1
  • zinc finger, MIZ-type containing 3
  • ZMIZ3

Background

The PIAS proteins (protein inhibitor of activated STAT) play a crucial role as transcriptional coregulators in various cellular pathways, including the STAT, p53 and the steroid hormone signaling pathway. The PIAS protein family includes at least five evolutionarily conserved genes, including PIAS1. The major function of the PIAS proteins is the control of gene transcription and can also act as small ubiquitin-like-modifier (SUMO) E3 ligases. PIAS1 binds specifically to STAT1, inhibiting STAT1-mediated gene activation and also binds to the Gu/RNA helicase II enzyme, leading to the proteolytic cleavage of Gu/RH-II. PIAS1 is a potent co-activator for CP2c-mediated alpha-globin expression in erythroid cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PIAS1 Antibody (NBP1-77158) (0)

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Review for PIAS1 Antibody (NBP1-77158) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-77158:
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Images Ratings Applications Species Date Details
Western Blot PIAS1 NBP1-77158
Enlarge 		3
reviewed by:
Verified Customer
 WB Human 09/25/2014
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Summary

ApplicationWestern Blot
Sample TestedTHP1 cells
SpeciesHuman

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Calculators

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Recent Reviews

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Western Blot PIAS1 NBP1-77158
Enlarge
 Verified Customer
09/25/2014
Application: WB
Species: Human
read this review

Bioinformatics

Gene Symbol PIAS1
Uniprot