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Axin-1 Antibody

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Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Balb/3T3 mouse embryonic fibroblast cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat Axin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3287) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Axin-1 at approximately 120 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Balb/3T3 mouse embryonic fibroblast cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.5 µg/mL of ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF3287-SP
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Axin-1 Antibody Summary

Immunogen
E. coli-derived recombinant human Axin-1
Tyr210-Glu410
Accession # O15169
Specificity
Detects human, mouse and rat Axin-1 in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
AXIN1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/mL
Publications
Read Publications using
AF3287 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Axin-1 Antibody

  • axin 1
  • Axin1
  • Axin-1
  • AXINaxis inhibitor 1
  • Axis inhibition protein 1
  • fused, mouse, homolog of
  • hAxin
  • MGC52315

Background

Axin-1 is a negative regulator of the Wnt signaling pathway, which is involved in regulation of embryonic axis formation in vertebrates. Axin-1 binds to beta -catenin and glycogen synthase kinase-3 (GSK-3) to promote beta -catenin phosphorylation and subsequent degradation. Axin-1 also binds to the adenomatous polyposis coli (APC) gene, a known tumor suppressor that also regulates beta -catenin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Axin-1 Antibody (AF3287)(15)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ICC, Immunoprecipitation, Western Blot.


Filter By Application
ICC
(1)
Immunoprecipitation
(1)
Western Blot
(8)
All Applications
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Human
(7)
Mouse
(3)
All Species
Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using AF3287 Applications Species
Lan Jiang, Qianhong Yang, Jianjun Gao, Jiahong Yang, Jiaqi He, Hong Xin et al. BK Channel Deficiency in Osteoblasts Reduces Bone Formation via the Wnt/ beta -Catenin Pathway Molecules and Cells 2021-08-31 [PMID: 34385407]
W Wang, P Liu, M Lavrijsen, S Li, R Zhang, S Li, WS van de Gee, HJG van de Wer, MP Peppelenbo, R Smits Evaluation of AXIN1 and AXIN2 as targets of tankyrase inhibition in hepatocellular carcinoma cell lines Scientific Reports, 2021-04-02;11(1):7470. 2021-04-02 [PMID: 33811251] (ICC, Western Blot, Human) ICC, Western Blot Human
TW Parker, KL Neufeld APC controls Wnt-induced beta-catenin destruction complex recruitment in human colonocytes Sci Rep, 2020-02-19;10(1):2957. 2020-02-19 [PMID: 32076059] (Western Blot, Human) Western Blot Human
C Dittfeld, G Reimann, A Mieting, P Büttner, A Jannasch, K Plötze, G Steiner, SM Tugtekin, K Matschke Treatment with XAV-939 prevents in vitro calcification of human valvular interstitial cells PLoS ONE, 2018-12-07;13(12):e0208774. 2018-12-07 [PMID: 30532256] (Western Blot, Human) Western Blot Human
K Saito-Diaz, H Benchabane, A Tiwari, A Tian, B Li, JJ Thompson, AS Hyde, LM Sawyer, JN Jodoin, E Santos, LA Lee, RJ Coffey, RD Beauchamp, CS Williams, AK Kenworthy, DJ Robbins, Y Ahmed, E Lee APC Inhibits Ligand-Independent Wnt Signaling by the Clathrin Endocytic Pathway Dev. Cell, 2018-03-12;44(5):566-581.e8. 2018-03-12 [PMID: 29533772] (Western Blot, Mouse) Western Blot Mouse
Lebensohn A, Dubey R, Neitzel L, Tacchelly-Benites O, Yang E, Marceau C, Davis E, Patel B, Bahrami-Nejad Z, Travaglini K, Ahmed Y, Lee E, Carette J, Rohatgi R Comparative genetic screens in human cells reveal new regulatory mechanisms in WNT signaling. Elife, 2016-12-20;5(0):. 2016-12-20 [PMID: 27996937] (Western Blot, Mouse) Western Blot Mouse
Zeinab Anvarian, Hisashi Nojima, Eline C van Kappel, Tobias Madl, Maureen Spit, Martin Viertler et al. Axin cancer mutants form nanoaggregates to rewire the Wnt signaling network Nature Structural & Molecular Biology 2016-04-01 [PMID: 26974125]
Estefania Martino-Echarri, Mariana G. Brocardo, Kate M. Mills, Beric R. Henderson Tankyrase Inhibitors Stimulate the Ability of Tankyrases to Bind Axin and Drive Assembly of beta -Catenin Degradation-Competent Axin Puncta PLOS ONE 2016-03-01 [PMID: 26930278]
Yu N, Kakunda M, Pham V, Lill J, Du P, Wongchenko M, Yan Y, Firestein R, Huang X HSP105 recruits protein phosphatase 2A to dephosphorylate beta-catenin. Mol Cell Biol, 2015-02-02;35(8):1390-400. 2015-02-02 [PMID: 25645927] (Immunoprecipitation, Human) Immunoprecipitation Human
Maria Gagliardi, Ana Hernandez, Ian J. McGough, Jean-Paul Vincent Inhibitors of endocytosis prevent Wnt/Wingless signalling by reducing the level of basal beta –Catenin/Armadillo Journal of Cell Science 2014-11-15 [PMID: 25236598]
Show All 15 Publications.

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Bioinformatics

Gene Symbol AXIN1
Uniprot