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PIAS1 Antibody [Janelia Fluor® 525]

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Janelia Fluor 525

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-77158AF350
Alexa Fluor 405
NBP1-77158AF405
Alexa Fluor 488
NBP1-77158AF488
Alexa Fluor 532
NBP1-77158AF532
Alexa Fluor 594
NBP1-77158AF594
Alexa Fluor 647
NBP1-77158AF647
Alexa Fluor 700
NBP1-77158AF700
Alexa Fluor 750
NBP1-77158AF750
Biotin
NBP1-77158B
CoraFluor 1
NBP1-77158CL1
DyLight 350
NBP1-77158UV
DyLight 405
NBP1-77158V
DyLight 488
NBP1-77158G
DyLight 550
NBP1-77158R
DyLight 594
NBP1-77158DL594
DyLight 650
NBP1-77158C
DyLight 680
NBP1-77158FR
DyLight 755
NBP1-77158IR
FITC
NBP1-77158F
HRP
NBP1-77158H
Janelia Fluor 549
NBP1-77158JF549
Janelia Fluor 585
NBP1-77158JF585
Janelia Fluor 635
NBP1-77158JF635
Janelia Fluor 646
NBP1-77158JF646
Janelia Fluor 669
NBP1-77158JF669
PerCP
NBP1-77158PCP
Unconjugated
NBP1-77158
mFluor Violet 450 SE
NBP1-77158MFV450
mFluor Violet 500 SE
NBP1-77158MFV500
mFluor Violet 610 SE
NBP1-77158MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP1-77158
BSA Free
NBP1-77158
BSA Free
NBP1-77158-0.025mg
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PIAS1 Antibody [Janelia Fluor® 525] Summary

Immunogen
Antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human PIAS1. The immunogen is located within the last 50 amino acids of PIAS1. Amino Acid Squence: SNSLRESHSHTVTNR
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PIAS1
Purity
Peptide affinity purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Peptide affinity purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for PIAS1 Antibody [Janelia Fluor® 525]

  • AR interacting protein
  • DDXBP1
  • DDXBP1MGC141878
  • DEAD/H (Asp-Glu-Ala-Asp/His) box binding protein 1
  • DEAD/H box-binding protein 1
  • E3 SUMO-protein ligase PIAS1
  • GBPRNA helicase II-binding protein
  • GU/RH-II
  • Gu-binding protein
  • MGC141879
  • PIAS1
  • Protein inhibitor of activated STAT protein 1
  • protein inhibitor of activated STAT, 1
  • protein inhibitor of activated STAT-1
  • zinc finger, MIZ-type containing 3
  • ZMIZ3

Background

PIAS1 encodes a member of the mammalian PIAS [protein inhibitor of activated STAT-1 (signal transducer and activator of transcription-1)] family. This member contains a putative zinc-binding motif and a highly acidic region. It inhibits STAT1-mediated gene activation and the DNA binding activity, binds to Gu protein/RNA helicase II/DEAD box polypeptide 21, and interacts with androgen receptor (AR). It functions in testis as a nuclear receptor transcriptional coregulator and may have a role in AR initiation and maintenance of spermatogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PIAS1