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RUNX3/CBFA3 Antibody

Images

 
Western Blot: RUNX3/CBFA3 Antibody [NBP2-38863] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line SK-MEL-30
Orthogonal Strategies: Immunohistochemistry-Paraffin: RUNX3/CBFA3 Antibody [NBP2-38863] - Staining in human lymph node and cerebral cortex tissues using anti-RUNX3 antibody. Corresponding RUNX3 RNA-seq data are ...read more
Immunohistochemistry-Paraffin: RUNX3/CBFA3 Antibody [NBP2-38863] - Staining of human cerebral cortex shows low expression as expected.
Immunohistochemistry-Paraffin: RUNX3/CBFA3 Antibody [NBP2-38863] - Staining of human lymph node shows high expression.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

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RUNX3/CBFA3 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: DQTKPFPDRFGDLERLRMRVTPSTPSPRGSLSTTSHFSSQPQTPIQGTSELNP
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RUNX3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
RUNX3/CBFA3 Protein (NBP2-38863PEP)
Publications
Read Publication using
NBP2-38863 in the following applications:

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Rat (87%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for RUNX3/CBFA3 Antibody

  • Acute myeloid leukemia 2 protein
  • AML2
  • AML2SL3/AKV core-binding factor alpha C subunit
  • CBFA3
  • CBFA3MGC16070
  • CBF-alpha-3
  • PEA2 alpha C
  • PEA2-alpha C
  • PEBP2 alpha C
  • PEBP2A3
  • PEBP2A3FLJ34510
  • PEBP2AC
  • PEBP2-alpha C
  • runt domain, alpha subunit 3
  • runt-related transcription factor 3
  • RUNX3
  • SL3-3 enhancer factor 1 alpha C subunit
  • transcription factor AML2

Background

RUNX3 binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, lck, IL-3 and GM-CSF promoters. RUNX3 consists of a heterodimer of an alpha and a beta subunit. The alpha subunit binds DNA as a monomer through the Runt domain. DNA-binding is increased by heterodimerization.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RUNX3/CBFA3 Antibody (NBP2-38863)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: IHC-Fr.


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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol RUNX3
Uniprot