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Neurogenin-1 Antibody (OTI3F9)

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Western Blot: Neurogenin-1 Antibody (OTI3F9) [NBP1-47871] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Neurogenin 1 monoclonal antibody.
Western Blot: Neurogenin-1 Antibody (OTI3F9) [NBP1-47871] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Neurogenin 1 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: Neurogenin-1 Antibody (OTI3F9) [NBP1-47871] - COS7 cells transiently transfected by pCMV6-ENTRY Neurogenin-1.
Immunohistochemistry-Paraffin: Neurogenin-1 Antibody (OTI3F9) [NBP1-47871] - Staining of Human prostate tissue.
Western Blot: Neurogenin-1 Antibody (OTI3F9) [NBP1-47871] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Neurogenin 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts ...read more
Immunohistochemistry-Paraffin: Neurogenin-1 Antibody (OTI3F9) [NBP1-47871] - Staining of Human colon tissue.
Immunohistochemistry-Paraffin: Neurogenin-1 Antibody (OTI3F9) [NBP1-47871] - Staining of Human Kidney tissue.
Immunohistochemistry-Paraffin: Neurogenin-1 Antibody (OTI3F9) [NBP1-47871] - Staining of Human lung tissue.
Immunohistochemistry-Paraffin: Neurogenin-1 Antibody (OTI3F9) [NBP1-47871] - Staining of Human Ovary tissue.
Immunohistochemistry-Paraffin: Neurogenin-1 Antibody (OTI3F9) [NBP1-47871] - Staining of Human pancreas tissue.
Immunoprecipitation: Neurogenin-1 Antibody (OTI3F9) [NBP1-47871] - Immunoprecipitation (IP) of NEUROG1 by using TrueMab monoclonal anti-NEUROG1 antibodies (Negative control: IP without adding anti-NEUROG1 antibody.). ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IP
Clone
OTI3F9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-72948AF350
Alexa Fluor 405
NBP2-72948AF405
Alexa Fluor 488
NBP2-72948AF488
Alexa Fluor 532
NBP2-72948AF532
Alexa Fluor 594
NBP2-72948AF594
Alexa Fluor 647
NBP2-72948AF647
Alexa Fluor 700
NBP2-72948AF700
Alexa Fluor 750
NBP2-72948AF750
Allophycocyanin
NBP2-72948APC
Allophycocyanin/Cy7
NBP2-72948APCCY7
Biotin
NBP2-72948B
DyLight 350
NBP2-72948UV
DyLight 405
NBP2-72948V
DyLight 488
NBP2-72948G
DyLight 550
NBP2-72948R
DyLight 594
NBP2-72948DL594
DyLight 650
NBP2-72948C
DyLight 680
NBP2-72948FR
DyLight 755
NBP2-72948IR
FITC
NBP2-72948F
HRP
NBP2-72948H
Janelia Fluor 549
NBP2-72948JF549
Janelia Fluor 646
NBP2-72948JF646
PE
NBP2-72948PE
PerCP
NBP2-72948PCP
mFluor Violet 450 SE
NBP2-72948MFV450
mFluor Violet 500 SE
NBP2-72948MFV500
mFluor Violet 610 SE
NBP2-72948MFV610
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-72948
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Neurogenin-1 Antibody (OTI3F9) Summary

Immunogen
Full length human recombinant protein of human NEUROG1 (NP_006152) produced in HEK293T cell.
Specificity
This antibody is specific for Homo sapiens neurogenin 1 (NEUROG1).
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NEUROG1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Immunoprecipitation 2-4ug/ml
  • Western Blot 1:500-1000
Theoretical MW
25.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Neurogenin-1 Antibody (OTI3F9)

  • AKA
  • BHLHA6
  • bHLHa6NGN-1
  • Class A basic helix-loop-helix protein 6
  • Math4C
  • NEUROD3
  • NEUROD3neurogenic differentiation 3
  • NEUROG1
  • Neurogenic basic-helix-loop-helix protein
  • Neurogenic differentiation factor 3
  • neurogenin 1
  • Neurogenin1
  • Neurogenin-1
  • NGN
  • ngn1

Background

Neurogenin 1 is the protein product of an activator-type bHLH gene (Ngn1). The activator-type bHLH genes (which also include Mash1 and Math) promote neuronal fate determination and induce expression of Notch ligands such as Delta which activate Notch signaling. The activator type bHLH genes are antagonized by repressor-type basic helix-loop-helix (bHLH) genes such as Hes1, 3 and 5. Thus, activator-type and repressor-type bHLH genes regulate each other, allowing only subsets of neural stem cells to undergo differentiation. Neurogenin 1 is thus one of the key regulators of neural stem cell differentiation, which is essential for generation of complex brain structures of appropriate size, shape and cell arrangement.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol NEUROG1
Entrez