![Western Blot: CACNA1G Antibody (S178A-9) [NBP2-59322] - Western Blot analysis of Rat brain membrane lysate showing detection of CACNA1G Calcium Channel protein using Mouse Anti-CACNA1G Calcium Channel Monoclonal Antibody, Clone S178A-9 (NBP2-59322). Primary Antibody: Mouse Anti-CACNA1G Calcium Channel Monoclonal Antibody (NBP2-59322) at 1:1000.](http://images.novusbio.com/fullsize/CACNA1G-Antibody-S178A-9-Western-Blot-NBP2-59322-img0005.jpg "Western Blot: CACNA1G Antibody (S178A-9) [NBP2-59322] - Western Blot analysis of Rat brain membrane lysate showing detection of CACNA1G Calcium Channel protein using Mouse Anti-CACNA1G Calcium Channel Monoclonal Antibody, Clone S178A-9 (NBP2-59322). Primary Antibody: Mouse Anti-CACNA1G Calcium Channel Monoclonal Antibody (NBP2-59322) at 1:1000.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | S178A-9 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | Fusion protein amino acids 2052-2172 (cytoplasmic C-terminus) of mouse Cav3.1 |
| Localization | Membrane |
| Specificity | Detects <200kDa. Does not cross-react with Cav3.2. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CACNA1G |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Reviewed Applications |
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| Storage | Store at -20C. |
| Buffer | PBS (pH 7.4), 50% Glycerol |
| Preservative | 0.09% Sodium Azide |
| Purity | Protein G purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Pauline Jeannot |
WB | Human | 11/16/2023 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for CACNA1G Antibody (NBP2-59322)Find related products by research area.
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| Gene Symbol | CACNA1G |
![Immunocytochemistry/Immunofluorescence: CACNA1G Antibody (S178A-9) [NBP2-59322] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-CACNA1G Monoclonal Antibody, Clone S178A-9 (NBP2-59322). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-CACNA1G Monoclonal Antibody (NBP2-59322) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) CACNA1G Antibody (D) Composite.](http://images.novusbio.com/fullsize/CACNA1G-Antibody-S178A-9-Immunocytochemistry-Immunofluorescence-NBP2-59322-img0006.jpg "Immunocytochemistry/Immunofluorescence: CACNA1G Antibody (S178A-9) [NBP2-59322] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-CACNA1G Monoclonal Antibody, Clone S178A-9 (NBP2-59322). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-CACNA1G Monoclonal Antibody (NBP2-59322) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) CACNA1G Antibody (D) Composite.")
![Immunocytochemistry/Immunofluorescence: CACNA1G Antibody (S178A-9) [NBP2-59322] - Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Cav3.1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Membrane, Cytoplasm, Nucleoplasm. Magnification: 60X.](http://images.novusbio.com/fullsize/CACNA1G-Antibody-S178A-9-Immunocytochemistry-Immunofluorescence-NBP2-59322-img0002.jpg "Immunocytochemistry/Immunofluorescence: CACNA1G Antibody (S178A-9) [NBP2-59322] - Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Cav3.1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Membrane, Cytoplasm, Nucleoplasm. Magnification: 60X.")
![Immunocytochemistry/Immunofluorescence: CACNA1G Antibody (S178A-9) [NBP2-59322] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-CACNA1G Monoclonal Antibody, Clone S178A-9 (NBP2-59322). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-CACNA1G Monoclonal Antibody (NBP2-59322) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Membrane, Cytoplasm, Nucleoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) CACNA1G Antibody. (D) Composite.](http://images.novusbio.com/fullsize/CACNA1G-Antibody-S178A-9-Immunocytochemistry-Immunofluorescence-NBP2-59322-img0007.jpg "Immunocytochemistry/Immunofluorescence: CACNA1G Antibody (S178A-9) [NBP2-59322] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-CACNA1G Monoclonal Antibody, Clone S178A-9 (NBP2-59322). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-CACNA1G Monoclonal Antibody (NBP2-59322) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Membrane, Cytoplasm, Nucleoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) CACNA1G Antibody. (D) Composite.")
![Western Blot: Mouse Monoclonal CACNA1G Antibody (S178A-9) [NBP2-59322] - Western blot using lysates from human primary astrocytes or IGR39 melanoma cell line. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp2-59322_mouse-monoclonal-cacna1g-antibody-s178a-9-western-blot-1122023151422..png "Western Blot: Mouse Monoclonal CACNA1G Antibody (S178A-9) [NBP2-59322] - Western blot using lysates from human primary astrocytes or IGR39 melanoma cell line. Image from a verified customer review.")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")
