Reactivity | HuSpecies Glossary |
Applications | Binding Activity, Enzyme Activity |
Format | Carrier-Free |
Additional Information | Biotinylated, His-tag, Avi-tag |
||||||
Details of Functionality | Measured by its ability to cleave the fluorogenic peptide substrate, Ala-7-amido-4-methylcoumarin (Ala-AMC). The specific activity is >5000 pmol/min/μg, as measured under the described conditions. Measured by its binding ability in a functional ELISA. Biotinylated
Recombinant Human Aminopeptidase N/CD13 His-tag Avi-tag (Catalog # AVI3815) binds
Human Aminopeptidase N/CD13 Antibody (Catalog #
AF3815) with an ED50
of 1.80-18.0 ng/mL. |
||||||
Source | Chinese Hamster Ovary cell line, CHO-derived human Aminopeptidase N/CD13 protein
|
||||||
N-terminal Sequence | Lys69 |
||||||
Structure / Form | Biotinylated via Avi-tag |
||||||
Protein/Peptide Type | Recombinant Enzymes |
||||||
Purity | >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
||||||
Endotoxin Note | <0.10 EU per 1 μg of the protein by the LAL method. |
Dilutions |
|
Theoretical MW | 105 kD. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
SDS-PAGE | 116-130 kDa, under reducing conditions. |
Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
|||
Buffer | Supplied as a 0.2 μm filtered solution in MES and NaCl. |
|||
Purity | >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
|||
Assay Procedure |
*Adjusted for Substrate Blank **Derived using calibration standard 7-Amino-4-Methyl Coumarin.
|
Aminopeptidase N (APN) is a type II transmembrane zinc-dependent metalloprotease that is widely expressed in many cells, tissues, and species. APN forms an active homodimer where each monomer contains a short cytoplasmic tail, a transmembrane region, and an extracellular domain with a Ser/Thr rich region and a metalloprotease catalytic domain (1,2). APN is also known as leukocyte surface differentiation antigen CD13, microsomal aminopeptidase, alanyl aminopeptidase, and aminopeptidase M (1-3). The protein cleaves the N-terminal neutral amino acids from bioactive peptides like neuropeptides and immunomodulatory peptides including oxytocin, vasopressin, angiotensin III, and tuftsin resulting in their degradation or modulation (2,4). Consequently, APN is implicated to play many biological roles within the immune system, angiogenesis, mestastasis, and cancer (2). Both APN expression and activity are altered in cancer making it a marker and a pharmacological intervention target (5,6). It is targeted in CAR T therapies for cancers such as B-cell lymphoma and multiple myeloma (2,7). In addition, APN serves as a receptor for several human viruses such as coronavirus 229E (8) and human cytomegalovirus making it of interest as a target in these fields (9,10).
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.