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CD7 Antibody (5B10E3) - BSA Free

Images

 
Western Blot: CD7 Antibody (5B10E3) [NBP2-37368] - Western blot analysis using CD7 mAb against HEK293 (1) and CD7 (AA: 72-175)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry: CD7 Antibody (5B10E3) [NBP2-37368] - Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues using CD7 mouse mAb with DAB staining.
Western Blot: CD7 Antibody (5B10E3) [NBP2-37368] - Western blot analysis using CD7 mAb against human CD7 recombinant protein. (Expected MW is 36.6 kDa)
ELISA: CD7 Antibody (5B10E3) [NBP2-37368] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Imaging Mass Cytometry: CD7 Antibody (5B10E3) [NBP2-37368] - FFPE human tonsil tissue, CD7 conjugated to 173Yb. IMC image submitted by a verified customer review.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, IHC, CyTOF-ready, MS
Clone
5B10E3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

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CD7 Antibody (5B10E3) - BSA Free Summary

Immunogen
Purified recombinant fragment of human CD7 (AA: 72-175) expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CD7
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Imaging Mass Cytometry
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
Application Notes
This antibody is Cytof ready. This CD7 Antibody (5B10E3) is validated for Imaging Mass Cytometry from a verified customer review.
Theoretical MW
25.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-37368 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for CD7 Antibody (5B10E3) - BSA Free

  • CD7 antigen (p41)
  • CD7 antigen
  • CD7 molecule
  • CD7
  • GP40T-cell surface antigen Leu-9
  • LEU-9
  • T-cell antigen CD7
  • T-cell leukemia antigen
  • Tp40
  • TP41
  • TP41p41 protein

Background

CD7, also known as gp40, is a 40 kD type I transmembrane glycoprotein member of the immunoglobulin superfamily. It is found on T cells, NK cells, thymocytes, hematopoietic progenitors, and monocytes (weakly). CD7 is also expressed on acute lymphocytic leukemia (ALL). CD7 crosslinking induces a calcium flux in T lymphocytes, presumably as a result of cytoplasmic domain association with PI3-kinase. CD7 co-stimulation can induce cytokine secretion and modulate cellular adhesion.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD7 Antibody (NBP2-37368) (0)

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Review for CD7 Antibody (NBP2-37368) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-37368:
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IMC
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
  5
reviewed by:
Alicia Gamboa
IMC Human 04/09/2021
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Summary

ApplicationImaging Mass Cytometry
Sample TestedHuman Tonsil tissue
SpeciesHuman
Lot130225

Product General Protocols

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FAQs for CD7 Antibody (NBP2-37368) (0)

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Secondary Antibodies

 

Isotype Controls

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Alicia Gamboa
04/09/2021
Application: IMC
Species: Human

Bioinformatics

Gene Symbol CD7