![Western Blot: TdT Antibody [NBP2-31368] - WB detection of TDT protein in (A) lysate of MOLT4 human leukemia cell line and (B) partial recombinant protein by using TDT antibody. Primary antibody concentration used: 1 ug/ml for Molt4 lysate, 0.05 ug/ml for recombinant protein.](http://images.novusbio.com/fullsize/TdT-Antibody-Western-Blot-NBP2-31368-img0001.jpg "Western Blot: TdT Antibody [NBP2-31368] - WB detection of TDT protein in (A) lysate of MOLT4 human leukemia cell line and (B) partial recombinant protein by using TDT antibody. Primary antibody concentration used: 1 ug/ml for Molt4 lysate, 0.05 ug/ml for recombinant protein.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1.0 mg/ml |
| Immunogen | Partial recombinant protein made to an internal sequence of human TdT (between residues 50-300) [UniProt# P04053] |
| Localization | Nucleus (high), Cytoplasm (very low) |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | DNTT |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | TdT is a 509 amino acids long protein with predicted molecular weight of 58.5 kDa, and it localizes mainly to the nucleus with low amounts in cytoplasm of the cells. In our Western blot validation with MOLT4 lysate, this TdT antibody detected a single specific band at ~66 kDa position. In our immunohistochemistry validation on formalin fixed paraffin embedded tissue sections, this antibody developed a strong nuclear/ weak cytoplasmic staining in normal lung and hepatocellular carcinoma of human. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Theoretical MW | 58.5 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Protein A purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for TdT Antibody (NBP2-31368)Find related products by research area.
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TdT (Terminal Deoxynucleotidyl Transferase) The enzyme family of DNA polymerases plays a fundamental role in the replication, repair, and recombination of nucleic acid. Its members include DNA Polymerase b (Pol b), DNA Polymerase g (Pol g), and DNA Polymerase m (Pol m). TdT is a very unique and... Read full blog post. |
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![Immunocytochemistry/Immunofluorescence: TdT Antibody [NBP2-31368] - TdT antibody was tested in A431 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). An antibody concentration of 0.01 ug/ml was used. Image objective 40x.](http://images.novusbio.com/fullsize/TdT-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-31368-img0006.jpg "Immunocytochemistry/Immunofluorescence: TdT Antibody [NBP2-31368] - TdT antibody was tested in A431 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). An antibody concentration of 0.01 ug/ml was used. Image objective 40x.")
![Immunohistochemistry-Paraffin: TdT Antibody [NBP2-31368] - IHC-P analysis of TdT protein in a section of human hepatocellular carcinoma (HCC) using TdT antibody at a concentration of 10 ug/ml. The HCC cells depicted strong nuclear / weak cytoplasmic positivity for TdT immunostaining.](http://images.novusbio.com/fullsize/TdT-Antibody-Immunohistochemistry-Paraffin-NBP2-31368-img0003.jpg "Immunohistochemistry-Paraffin: TdT Antibody [NBP2-31368] - IHC-P analysis of TdT protein in a section of human hepatocellular carcinoma (HCC) using TdT antibody at a concentration of 10 ug/ml. The HCC cells depicted strong nuclear / weak cytoplasmic positivity for TdT immunostaining.")
![Immunohistochemistry-Paraffin: TdT Antibody [NBP2-31368] - IHC-P analysis of TdT protein in a section of human lung using TdT antibody at a concentration of 10 ug/ml. The alveolar cells of the lungs showed strong nuclear / weak cytoplasmic positivity for TdT immunostaining.](http://images.novusbio.com/fullsize/TdT-Antibody-Immunohistochemistry-Paraffin-NBP2-31368-img0002.jpg "Immunohistochemistry-Paraffin: TdT Antibody [NBP2-31368] - IHC-P analysis of TdT protein in a section of human lung using TdT antibody at a concentration of 10 ug/ml. The alveolar cells of the lungs showed strong nuclear / weak cytoplasmic positivity for TdT immunostaining.")
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![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")