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Prohibitin 2 Antibody

Images

 
Western Blot: Prohibitin 2 Antibody [NBP1-98312] - WB Suggested Anti-Phb2 Antibody. Positive Control: Lane 1: 80ug mouse brain extractLane 2: 80ug rat brain extract. Primary Antibody Dilution : 1:500. Secondary Antibody ...read more
Western Blot: Prohibitin 2 Antibody [NBP1-98312] - Mouse Heart Lysate 1.0ug/ml, Gel Concentration: 12%
Immunoprecipitation: Prohibitin 2 Antibody [NBP1-98312] - NT2 CELL/BRAIN TISSUE

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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Prohibitin 2 Antibody Summary

Description
The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.
Immunogen
The immunogen for this antibody is prohibitin 2 - middle region. Peptide sequence REYTAAVEAKQVAQQEAQRAQFLVEKAKQEQRQKIVQAEGEAEAAKMLGE. The peptide sequence for this immunogen was taken from within the described region.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PHB2
Purity
Affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1.0 ug/ml
Theoretical MW
33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 2% Sucrose
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Affinity purified

Alternate Names for Prohibitin 2 Antibody

  • BAP
  • Bap37
  • BCAP37
  • B-cell associated protein
  • D-Prohibitin
  • MGC117268
  • p22
  • PHB2
  • PNAS-141
  • Prohibitin 2
  • prohibitin-2
  • REA
  • REAB-cell receptor-associated protein BAP37
  • Repressor of estrogen receptor activity

Background

REA, or repressor of estrogen receptor activity, (also known as BAP37, or B-cell receptor-associated protein) is a 37 kD erythrocyte band 7 integral membrane protein that contains an SPFH domain. It is located in the inner mitochondrial membrane that selectively potentiates the inhibitory effectiveness of antiestrogen-occupied ER. REA plays a possible role in cell aging and acts as a mitochondrial protein chaperone. This protein has been shown to interact with the estrogen receptor, prohibitin, and IgM.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PHB2
Uniprot