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Methionine Aminopeptidase 2/METAP2 Antibody

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Western Blot: Methionine Aminopeptidase 2/METAP2 Antibody [NBP3-05513] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-METAP2 antibody. Remaining relative ...read more
Immunocytochemistry/ Immunofluorescence: Methionine Aminopeptidase 2/METAP2 Antibody [NBP3-05513] - Staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.
Immunohistochemistry-Paraffin: Methionine Aminopeptidase 2/METAP2 Antibody [NBP3-05513] - Staining of human Testis shows strong cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: Methionine Aminopeptidase 2/METAP2 Antibody [NBP3-05513] - Staining of human lymph node shows strong cytoplasmic positivity in germinal center cells.
Immunohistochemistry-Paraffin: Methionine Aminopeptidase 2/METAP2 Antibody [NBP3-05513] - Staining of human Cervix shows moderate cytoplasmic positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: Methionine Aminopeptidase 2/METAP2 Antibody [NBP3-05513] - Staining of human Skeletal muscle shows very weak cytoplasmic positivity in myocytes.
Immunohistochemistry-Paraffin: Methionine Aminopeptidase 2/METAP2 Antibody [NBP3-05513] - Staining of human Tonsil shows strong cytoplasmic positivity in germinal center cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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Methionine Aminopeptidase 2/METAP2 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: SGSHLNGDLDPDDREEGAASTAEEAAKKKRRKKKKSKGPSAAGEQEPDKESGASVDEVARQLERSALEDKERDEDDEDGDGDGDGATGKKKK
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
METAP2
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF fixation permeabilization use PFA/Triton X-100.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (80%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.2, containing 40% glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for Methionine Aminopeptidase 2/METAP2 Antibody

  • Amp2
  • eIF-2-associated p67 homolog
  • Initiation factor 2-associated 67 kDa glycoprotein
  • MAP 2
  • MAP2
  • MetAP 2
  • METAP2
  • Methionine Aminopeptidase 2
  • methionyl aminopeptidase 2
  • MNPEP
  • p67
  • p67EC 3.4.11.18
  • p67eIF2
  • Peptidase M 2

Background

METAP2 is a member of the methionyl aminopeptidase family and encodes a protein that binds 2 cobalt or manganese ions. This protein functions both by protecting the alpha subunit of eukaryotic initiation factor 2 from inhibitory phosphorylation and by removing the amino-terminal methionine residue from nascent protein. Increased expression of this gene is associated with various forms of cancer and the anti-cancer drugs fumagillin and ovalicin inhibit the protein by irreversibly binding to its active site. A pseudogene of this gene is located on chromosome 2. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol METAP2